Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(vanillin synthesis)
(vanillin synthesis)
Line 70: Line 70:
! class='top' | date
! class='top' | date
|-
|-
-
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid||||108,8 ng/µl||August 4
+
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (C2)||||108,8 ng/µl||August 4
|-
|-
-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid||||113,9 ng/µl||August 4
+
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (C1)||||113,9 ng/µl||August 4
|-
|-
|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
|-
|-
-
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid||||101,3 ng/µl||August 5
+
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (A2)||||101,3 ng/µl||August 5
|-
|-
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid||||95,7 ng/µl||August 5
+
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (A1)||||95,7 ng/µl||August 5
|-
|-
|{{kulpart|BBa_I742142}}||pSB1A2||Sam8||purified plasmid||||81,5 ng/µl||August 5
|{{kulpart|BBa_I742142}}||pSB1A2||Sam8||purified plasmid||||81,5 ng/µl||August 5
|-
|-
-
|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid||||122,9 ng/µl||August 5
+
|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (B2)||||122,9 ng/µl||August 5
|-
|-
|{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||||||August 6
|{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||||||August 6

Revision as of 12:05, 12 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter region (1:200 adn 1:500)restricted with EcoRIjuly 29
/PCR BLR promoter region (360bp)4 microcentrifuge tubesjuly 30
Template:IGempart/kanamycine pasmide backbone taken from plates 13μl aug 11
Template:IGempart/constitutive promotortaken from plates 13μl aug 11
Template:IGempart/Exact blue light promotor cut with EcoRI and SpeI 26μl aug 12

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid (C2)108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid (C1)113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid (A2)101,3 ng/µlAugust 5
pSB1A2Sam8 + rbspurified plasmid (A1)95,7 ng/µlAugust 5
pSB1A2Sam8purified plasmid81,5 ng/µlAugust 5
pSB1A2COMTpurified plasmid (B2)122,9 ng/µlAugust 5
pSB1A2COMT + rbsplasmid from registryAugust 6
pSB1A2ech + rbspurified digest16,9 ng/µlAugust 6
fcs + rbspurified digest16,6 ng/µlAugust 6
pSB1A2sam5 + rbspurified digest3,1 ng/µlAugust 7
sam8 + rbspurified digest3,4 ng/µl August 7
pSB1A2fcs + rbspurified plasmid (fcs pl.)41 µl57,2 ng/µlAugust 12
pSB1A2Sam5 + rbspurified plasmid (sam5 pl.)43 µl76,0 ng/µlAugust 12
pSB1A2Sam8 + rbspurified plasmid (sam8 pl.)17 µl45,2 ng/µlAugust 12

vanillin receptor

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid101,3 ng/µlAugust 5

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline