Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→vanillin synthesis)
(→vanillin synthesis)
 
(37 intermediate revisions not shown)
Line 4: Line 4:
= Blue light receptor =
= Blue light receptor =
 +
{| class="Generic sortable"
{| class="Generic sortable"
! class='top' | part
! class='top' | part
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| MC4100 || / || strain of E.coli || || || || july 24
| MC4100 || / || strain of E.coli || || || || july 24
|-
|-
-
| 2171 || / ||forward primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM ||july 24
+
| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || 42,5µl || 20μM ||aug 10
|-
|-
-
| 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM || july 24
+
| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || 45µl || 20μM || aug 10
|-
|-
-
| 2171 || / || forward primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24
+
| {{kulpart|BBa_E0240}}||{{kulpart|pSB1A2}}||GFP + rbs + terminator||Miniprepped||100μl||85,6ng/μl||july 29
|-
|-
-
| 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24
+
| {{kulpart|BBa_E0240}}||{{kulpart|pSB1A2}}||GFP + rbs + terminator||restricted with EcoRI and XbaI|| 20μl ||||july 29
|-
|-
-
| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || 45µl || 20μM ||aug 10
+
| {{kulpart|BBa_K145015}}||{{kulpart|pSB1A2}}||GFP + LVA-tag + terminator||restricted with EcoRI and XbaI||30μl||29,3 ng/μl|| aug 14
|-
|-
-
| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || 45µl || 20μM || aug 10
+
| {{kulpart|pSB3K3}}|| {{kulpart|pSB3K3}} ||kanamycine plasmide backbone|| taken from plates || 13μl ||||aug 11
|-
|-
-
| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || || 100μM || aug 10
+
| {{kulpart|BBa_K238013}}||/||Exact blue light promotor ||pcr product|| 20μl || 123,3 ng/μl ||aug 13
|-
|-
-
| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || || 100μM || aug 10
+
| {{kulpart|BBa_J23101}}||/||constitutive promotor||taken from plates || 13μl ||||aug 11
|-
|-
-
| {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||restricted with EcoRI and XbaI||||||july 29
+
| {{kulpart|BBa_K238013}}||/||Exact blue light promotor ||cut with EcoRI and SpeI || 26μl || 27 ng/μl ||aug 12
|-
|-
-
| {{kulpart|BBa_K238000}}||/||BLR promoter region (1:200 adn 1:500)||restricted with EcoRI||||||july 29
+
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_E0240}}||{{kulpart|pSB1A3}}||LigA: Exact blue light promotor ligated with {{kulpart|BBa_E0240}} in {{kulpart|pSB1A2}} ||T4 DNA ligation || 18μl || ||aug 13
|-
|-
-
| {{kulpart|BBa_K238000}}||/||PCR BLR promoter region (360bp)||4 microcentrifuge tubes||||||july 30
+
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_K145015}}||{{kulpart|pSB1A3}}||Lig B: Exact blue light promotor ligated with {{kulpart|BBa_K145015}} in {{kulpart|pSB1A2}} ||T4 DNA ligation || 18μl || ||aug 14
|-
|-
-
| {{iGempart|BBa_pSB3K3-P1010}}|| pSB3K3 ||kanamycine plasmide backbone|| taken from plates || 13μl ||||aug 11
+
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_E0240}}||/||Lig A: Exact blue light promotor ligated with {{kulpart|BBa_E0240}} in {{kulpart|pSB1A2}} (1,3,7,9) ||cut with EcoRI and PstI || 4 x 18μl || ||aug 24
|-
|-
-
| {{iGempart|BBa_J23101}}||/||constitutive promotor||taken from plates || 13μl ||||aug 11
+
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_E0240}}||{{kulpart|pSB1A2}}||LigA: Exact blue light promotor ligated with {{kulpart|BBa_E0240}} in {{kulpart|pSB1A2}} ||Miniprep (4x)|| 20μl|| ||aug 18
|-
|-
-
| {{iGempart|BBa_K238013}}||/||Exact blue light promotor ||cut with EcoRI and SpeI || 26μl || 27 ng/μl ||aug 12
+
| {{kulpart|BBa_J23101}}||{{kulpart|pSB1A3}}||constitutive promotor||miniprep||10μl||141,2ng/μl||aug 14
|-
|-
-
| {{iGempart|BBa_K238013+ BBa_E0240}}||/||Exact blue light promotor ligated with E0240 in pSB1A2 ||T4 DNA ligation || 18μl || ||aug 13
+
| {{kulpart|BBa_J23101}}(A)||||constitutive promotor||restriction digest with EcoRI en SpeI|| 20μl ||20,8ng/μl||aug 24
|-
|-
-
| {{iGempart|BBa_K238013+ BBa_K145015}}||/||Exact blue light promotor ligated with K145015 in pSB1A2 ||T4 DNA ligation || 18μl || ||aug 14
+
| {{kulpart|BBa_J23101}}(B)||||constitutive promotor||restriction digest with EcoRI en SpeI|| 10μl ||12,8ng/μl||aug 24
 +
|-
 +
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_E0240}}||{{kulpart|pSB1A2}}||LigA (1): Exact blue light promotor ligated with {{kulpart|BBa_E0240}} in {{kulpart|pSB1A2}} ||Miniprep|| 45μl||110,6ng/μl||aug 31
 +
|-
 +
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_E0240}}||{{kulpart|pSB1A2}}||LigA (2): Exact blue light promotor ligated with {{kulpart|BBa_E0240}} in {{kulpart|pSB1A2}} ||Miniprep|| 43μl||76,7ng/μl||aug 31
 +
|-
 +
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_E0240}}||{{kulpart|pSB1A2}}||LigA (3): Exact blue light promotor ligated with {{kulpart|BBa_E0240}} in {{kulpart|pSB1A2}} ||Miniprep|| 44μl||84,8ng/μl||aug 31
 +
|-
 +
| {{kulpart|BBa_K238013}}+{{kulpart|BBa_E0240}}||{{kulpart|pSB1A2}}||LigA (4): Exact blue light promotor ligated with {{kulpart|BBa_E0240}} in {{kulpart|pSB1A2}} ||Miniprep|| 34μl||31,7ng/μl||aug 31
 +
|-
 +
| {{kulpart|BBa_E0240}}||pSB1A2||RBS + GFP + terminator||Miniprep||45μl||104,3ng/μl||aug 31
 +
|-
 +
| {{kulpart|BBa_J23101}} (A)||pSB1A2|| promotor||Miniprep||48μl||238,9ng/μl||sept 2
 +
|-
 +
| {{kulpart|BBa_J23101}} (B)||pSB1A2|| promotor||Miniprep||48μl||248,8ng/μl||sept 2
|}
|}
Line 65: Line 80:
= vanillin synthesis =
= vanillin synthesis =
 +
{| class="Generic sortable"
{| class="Generic sortable"
! class='top' | part
! class='top' | part
Line 74: Line 90:
! class='top' | date
! class='top' | date
|-
|-
-
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (oranje)||||108,8 ng/µl||August 4
+
|{{kulpart|BBa_I742115}}||{{kulpart|pSB1A2}}||fcs + rbs||purified plasmid (FCS 1)||13 µl||32.1 ng/µl||September 1
|-
|-
-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (oranje)||||113,9 ng/µl||August 4
+
|{{kulpart|BBa_I742115}}||{{kulpart|pSB1A2}}||fcs + rbs||purified plasmid (FCS 2)||28 µl||67.2 ng/µl||September 1
|-
|-
-
|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
+
|{{kulpart|BBa_I742115}}||{{kulpart|pSB1A2}}||fcs + rbs||purified plasmid (FCS 3)||28 µl||82.4 ng/µl||September 1
|-
|-
-
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (oranje)||||101,3 ng/µl||August 5
+
|{{kulpart|BBa_I742113}}||{{kulpart|pSB1A2}}||ech + rbs||purified plasmid (ECH 1)||15 µl||37.2 ng/µl||September 1
|-
|-
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (oranje)||||95,7 ng/µl||August 5
+
|{{kulpart|BBa_I742113}}||{{kulpart|pSB1A2}}||ech + rbs||purified plasmid (ECH 2)||28 µl||26.9 ng/µl||September 1
|-
|-
-
|{{kulpart|BBa_I742142}}||pSB1A2||Sam8 (b)||purified plasmid (oranje)||||81,5 ng/µl||August 5
+
|{{kulpart|BBa_I742113}}||{{kulpart|pSB1A2}}||ech + rbs||purified plasmid (ECH 3)||28 µl||28.0 ng/µl||September 1
|-
|-
-
|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (oranje)||||122,9 ng/µl||August 5
+
|{{kulpart|BBa_K238007}}||{{kulpart|pSB1A2}}||Sam5 + rbs||purified plasmid (SAM5 1)||19.6 µl||59.9 ng/µl||September 1
|-
|-
-
|{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||0 µl||||August 6
+
|{{kulpart|BBa_K238007}}||{{kulpart|pSB1A2}}||Sam5 + rbs||purified plasmid (SAM5 2)||28 µl||52.8 ng/µl||September 1
|-
|-
-
|||pSB1A2||ech + rbs||purified digest (C2)||||16,9 ng/µl||August 6
+
|{{kulpart|BBa_K238007}}||{{kulpart|pSB1A2}}||Sam5 + rbs||purified plasmid (SAM5 3)||13 µl||67.3 ng/µl||September 1
|-
|-
-
|||||fcs + rbs||purified digest (C1, FCS Rb)||||16,6 ng/µl||August 6
+
|{{kulpart|BBa_I742106}}||{{kulpart|pSB1A2}}||Sam8 + rbs||purified plasmid (SAM8 1)||12 µl||31.0 ng/µl||September 1
|-
|-
-
|||pSB1A2||sam5 + rbs||purified digest (A2)||||3,1 ng/µl||August 7
+
|{{kulpart|BBa_I742106}}||{{kulpart|pSB1A2}}||Sam8 + rbs||purified plasmid (SAM8 2)||8 µl||47.2 ng/µl||September 1
|-
|-
-
|||||sam8 + rbs||purified digest (A1)||||3,4 ng/µl ||August 7
+
|{{kulpart|BBa_I742106}}||{{kulpart|pSB1A2}}||Sam8 + rbs||purified plasmid (SAM8 3)||28 µl||42.9 ng/µl||September 1
|-
|-
-
|||pSB1A2||sam5 + sam8||ligation (lig. A)||||||August 11
+
|{{kulpart|BBa__B0015}}||{{kulpart|pSB1AK3}}||Terminator||Terminator, B0015 || ±20 µl||181,4|| September 3
|-
|-
-
|||pSB1A2||ech + fcs||ligation (lig.C)||||||August 11
+
|{{kulpart|BBa_I742106}}||{{kulpart|pSB1A2}}||sam8||SAM8 RD 3/9||28 µl ||8,2 ng/µl||September 3
|-
|-
-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs pl.)||29 µl||57,2 ng/µl||August 12
+
|{{kulpart|BBa_K238007}}||{{kulpart|pSB1A2}}||sam5||SAM5 RD 3/9 ||28 µl||1,4 ng/µl|| September 3
|-
|-
-
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 pl.)||31 µl||76,0 ng/µl||August 12
+
|{{kulpart|BBa_I742113}}||{{kulpart|pSB1A2}}||ech||ECH RD 3/9 ||23 µl||10,1 ng/µl|| September 3
|-
|-
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 pl.)||0 µl||45,2 ng/µl||August 12
+
|{{kulpart|BBa_I742115}}||{{kulpart|pSB1A2}}||fcs||FCS RD 3/9 ||4 µl||5,2 ng/µl|| September 3
|-
|-
-
|||||fcs + rbs||purified digest (F rd.)||18 µl||4,2 ng/µl||August 12
+
|{{kulpart|BBa_I742106}}||||sam8||SAM8 PCR||µl ||361.6 ng/µl||September 3
|-
|-
-
|||pSB1A2||sam5 + rbs||purified digest (5 rd.)||18 µl||7,3 ng/µl||August 12
+
|{{kulpart|BBa_K238007}}||||sam5||SAM5 PCR||µl||323.0 ng/µl|| September 3
|-
|-
-
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech pl.)||38 µl||72,7 ng/µl||August 13
+
|{{kulpart|BBa_I742113}}||||ech||ECH PCR||µl||236.5 ng/µl|| September 3
|-
|-
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 pl.)||38 µl||66,2 ng/µl||August 13
+
|{{kulpart|BBa_I742115}}||||fcs||FCS PCR||µl||321.4 ng/µl|| September 3
|-
|-
-
|||pSB1A2||sam5 + sam8||cells with sam5+sam8 (elek. A)||600 µl||||August 13
+
|||{{kulpart|pSB1A2}}||ech + fcs||Ligatie zonder terminator(EFS)|| µl||ng/µl|| September 3
|-
|-
-
|||pSB1A2||ech + fcs||cells with ech+fcs (elek. C)||600 µl||||August 13
+
|||{{kulpart|pSB1A2}}||ech + fcs||Ligatie met terminator(EFT) || µl||ng/µl|| September 3
|-
|-
-
|||||sam8 + rbs||purified digest (sam8 rd.)||16 µl||9,4 ng/µl||August 14
+
|||{{kulpart|pSB1A2}}||sam5 + sam8||Ligatie zonder terminator(58S)|| µl||ng/µl|| September 4
|-
|-
-
|||pSB1A2||ech + rbs||purified digest (ech rd.)||16 µl||13,6 ng/µl||August 14
+
|||{{kulpart|pSB1A2}}||sam5 + sam8||Ligatie met terminator(58T)|| µl||ng/µl|| September 4
|-
|-
-
|||pSB1A2||sam8 + Sam5||ligation (SAMS)||||||August 14
+
|||{{kulpart|pSB1A2}}||ech + fcs||Miniprepped Ligatie Jochem (EF1)||48 µl|| 115.5 ng/µl|| September 7
|-
|-
-
|||pSB1A2||ech + fcs||ligation (EF)||||||August 14
+
|||{{kulpart|pSB1A2}}||ech + fcs||Miniprepped Ligatie Jochem (EF3)||48 µl|| 95.1 ng/µl|| September 7
|-
|-
-
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech A)||36,8 µl||88,6 ng/µl||August 19
+
|{{kulpart|BBa_K238007}}||{{kulpart|pSB1A2}}||sam5||SAM5 purified||23 µl||10.0 ng/µl|| September 7
|-
|-
-
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech B)||48 µl||96,7 ng/µl||August 19
+
|||{{kulpart|pSB1A2}}||ech + fcs||Restrictie Ligatie EF - Jochem (EFX 3)|| 22 µl|| 4,3 ng/µl|| September 9
|-
|-
-
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech C)||48 µl||105,6 ng/µl||August 19
+
|{{kulpart|BBa__B0015}}||{{kulpart|pSB1AK3}}||TER||Restrictie TER - Jochem (TER RD)|| 24 µl|| 14,2 ng/µl|| September 10
|-
|-
-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs A)||30,3 µl||84,8 ng/µl||August 19
+
|||{{kulpart|pSB1A2}}||Sam5+Sam8||Restrictie Sam5/Sam8 - Jochem E/S (SAMS1)|| 28 µl|| X ng/µl|| September 10
|-
|-
-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs B)||48 µl||113,4 ng/µl||August 19
 
-
|-
 
-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs C)||48 µl||90,3 ng/µl||August 19
 
-
|-
 
-
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 A)||37,4 µl||94,6 ng/µl||August 19
 
-
|-
 
-
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 B)||48 µl||125,0 ng/µl||August 19
 
-
|-
 
-
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 C)||48 µl||104,2 ng/µl||August 19
 
-
|-
 
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 A)||21,9 µl||58,2 ng/µl||August 19
 
-
|-
 
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||48 µl||63,2 ng/µl||August 19
 
|}
|}
= vanillin receptor =
= vanillin receptor =
 +
{| class="Generic sortable"
{| class="Generic sortable"
! class='top' | part
! class='top' | part
Line 170: Line 174:
| 2238 || / || reverse primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3
| 2238 || / || reverse primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3
|-
|-
-
| 2242 || / || forward primer for {{kulpart|BBa_K238009}} || || || 20μM ||August 3
+
| 2242 || / || forward primer for {{kulpart|BBa_K238009}} || || || 20μM || August 3
|-
|-
| 2243 || / || reverse primer for {{kulpart|BBa_K238009}} || || || 20μM || August 3
| 2243 || / || reverse primer for {{kulpart|BBa_K238009}} || || || 20μM || August 3
Line 186: Line 190:
| 2245 || / || reverse primer for {{kulpart|BBa_K238010}} || || || 100μM || August 3
| 2245 || / || reverse primer for {{kulpart|BBa_K238010}} || || || 100μM || August 3
|-
|-
-
| 2246 || / || forward primer for {{kulpart|BBa_K238011}} || || || 20μM ||August 3
+
| 2246 || / || forward primer for {{kulpart|BBa_K238011}} || || || 20μM || August 3
|-
|-
| 2247 || / || reverse primer for {{kulpart|BBa_K238011}} || || || 20μM || August 3
| 2247 || / || reverse primer for {{kulpart|BBa_K238011}} || || || 20μM || August 3
Line 225: Line 229:
|-
|-
| 2295 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 100μM || August 8
| 2295 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 100μM || August 8
-
|-}
+
|}
= miscellaneous =
= miscellaneous =
 +
{| class="Generic sortable"
{| class="Generic sortable"
! class='top' | part
! class='top' | part
Line 239: Line 244:
|dNTP mix||||ATP, GTP, TTP, CTP||||94||10μM||aug 10
|dNTP mix||||ATP, GTP, TTP, CTP||||94||10μM||aug 10
|-
|-
-
|{{kulpart|J23110}}||pSB1A2||medium constitutieve promoter||||||||july 23
+
|{{kulpart|BBa_J23110}}||{{kulpart|pSB1A2}}||medium constitutieve promoter||||||||july 23
|-
|-
|ampicilline||||||||||||
|ampicilline||||||||||||
|}
|}

Latest revision as of 11:20, 14 September 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2260 / forward primer for 42,5µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
GFP + rbs + terminatorMiniprepped100μl85,6ng/μljuly 29
GFP + rbs + terminatorrestricted with EcoRI and XbaI 20μl july 29
GFP + LVA-tag + terminatorrestricted with EcoRI and XbaI30μl29,3 ng/μl aug 14
kanamycine plasmide backbone taken from plates 13μl aug 11
/Exact blue light promotor pcr product 20μl 123,3 ng/μl aug 13
/constitutive promotortaken from plates 13μl aug 11
/Exact blue light promotor cut with EcoRI and SpeI 26μl 27 ng/μl aug 12
+LigA: Exact blue light promotor ligated with in T4 DNA ligation 18μl aug 13
+Lig B: Exact blue light promotor ligated with in T4 DNA ligation 18μl aug 14
+/Lig A: Exact blue light promotor ligated with in (1,3,7,9) cut with EcoRI and PstI 4 x 18μl aug 24
+LigA: Exact blue light promotor ligated with in Miniprep (4x) 20μl aug 18
constitutive promotorminiprep10μl141,2ng/μlaug 14
(A)constitutive promotorrestriction digest with EcoRI en SpeI 20μl 20,8ng/μlaug 24
(B)constitutive promotorrestriction digest with EcoRI en SpeI 10μl 12,8ng/μlaug 24
+LigA (1): Exact blue light promotor ligated with in Miniprep 45μl110,6ng/μlaug 31
+LigA (2): Exact blue light promotor ligated with in Miniprep 43μl76,7ng/μlaug 31
+LigA (3): Exact blue light promotor ligated with in Miniprep 44μl84,8ng/μlaug 31
+LigA (4): Exact blue light promotor ligated with in Miniprep 34μl31,7ng/μlaug 31
pSB1A2RBS + GFP + terminatorMiniprep45μl104,3ng/μlaug 31
(A)pSB1A2 promotorMiniprep48μl238,9ng/μlsept 2
(B)pSB1A2 promotorMiniprep48μl248,8ng/μlsept 2

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
fcs + rbspurified plasmid (FCS 1)13 µl32.1 ng/µlSeptember 1
fcs + rbspurified plasmid (FCS 2)28 µl67.2 ng/µlSeptember 1
fcs + rbspurified plasmid (FCS 3)28 µl82.4 ng/µlSeptember 1
ech + rbspurified plasmid (ECH 1)15 µl37.2 ng/µlSeptember 1
ech + rbspurified plasmid (ECH 2)28 µl26.9 ng/µlSeptember 1
ech + rbspurified plasmid (ECH 3)28 µl28.0 ng/µlSeptember 1
Sam5 + rbspurified plasmid (SAM5 1)19.6 µl59.9 ng/µlSeptember 1
Sam5 + rbspurified plasmid (SAM5 2)28 µl52.8 ng/µlSeptember 1
Sam5 + rbspurified plasmid (SAM5 3)13 µl67.3 ng/µlSeptember 1
Sam8 + rbspurified plasmid (SAM8 1)12 µl31.0 ng/µlSeptember 1
Sam8 + rbspurified plasmid (SAM8 2)8 µl47.2 ng/µlSeptember 1
Sam8 + rbspurified plasmid (SAM8 3)28 µl42.9 ng/µlSeptember 1
TerminatorTerminator, B0015 ±20 µl181,4 September 3
sam8SAM8 RD 3/928 µl 8,2 ng/µlSeptember 3
sam5SAM5 RD 3/9 28 µl1,4 ng/µl September 3
echECH RD 3/9 23 µl10,1 ng/µl September 3
fcsFCS RD 3/9 4 µl5,2 ng/µl September 3
sam8SAM8 PCRµl 361.6 ng/µlSeptember 3
sam5SAM5 PCRµl323.0 ng/µl September 3
echECH PCRµl236.5 ng/µl September 3
fcsFCS PCRµl321.4 ng/µl September 3
ech + fcsLigatie zonder terminator(EFS) µlng/µl September 3
ech + fcsLigatie met terminator(EFT) µlng/µl September 3
sam5 + sam8Ligatie zonder terminator(58S) µlng/µl September 4
sam5 + sam8Ligatie met terminator(58T) µlng/µl September 4
ech + fcsMiniprepped Ligatie Jochem (EF1)48 µl 115.5 ng/µl September 7
ech + fcsMiniprepped Ligatie Jochem (EF3)48 µl 95.1 ng/µl September 7
sam5SAM5 purified23 µl10.0 ng/µl September 7
ech + fcsRestrictie Ligatie EF - Jochem (EFX 3) 22 µl 4,3 ng/µl September 9
TERRestrictie TER - Jochem (TER RD) 24 µl 14,2 ng/µl September 10
Sam5+Sam8Restrictie Sam5/Sam8 - Jochem E/S (SAMS1) 28 µl X ng/µl September 10

vanillin receptor

part plasmid description status μl left concentration date
2235 / forward primer for 20μM August 3
2238 / reverse primer for 20μM August 3
2235 / forward primer for 100μM August 3
2238 / reverse primer for 100μM August 3
2242 / forward primer for 20μM August 3
2243 / reverse primer for 20μM August 3
2242 / forward primer for 100μM August 3
2243 / reverse primer for 100μM August 3
2244 / forward primer for 20μM August 3
2245 / reverse primer for 20μM August 3
2244 / forward primer for 100μM August 3
2245 / reverse primer for 100μM August 3
2246 / forward primer for 20μM August 3
2247 / reverse primer for 20μM August 3
2246 / forward primer for 100μM August 3
2247 / reverse primer for 100μM August 3
2288 / forward primer for mutagenesis for 20μM August 8
2289 / reverse primer for mutagenesis for 20μM August 8
2288 / forward primer for mutagenesis for 100μM August 8
2289 / reverse primer for mutagenesis for 100μM August 8
2290 / forward primer for mutagenesis for 20μM August 8
2291 / reverse primer for mutagenesis for 20μM August 8
2290 / forward primer for mutagenesis for 100μM August 8
2291 / reverse primer for mutagenesis for 100μM August 8
2292 / forward primer for mutagenesis for 20μM August 8
2293 / reverse primer for mutagenesis for 20μM August 8
2292 / forward primer for mutagenesis for 100μM August 8
2293 / reverse primer for mutagenesis for 100μM August 8
2294 / forward primer for mutagenesis for 20μM August 8
2295 / reverse primer for mutagenesis for 20μM August 8
2294 / forward primer for mutagenesis for 100μM August 8
2295 / reverse primer for mutagenesis for 100μM August 8

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
medium constitutieve promoterjuly 23
ampicilline
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