Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→vanillin synthesis)
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|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
|-
|-
-
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||received from Edinburgh||||||August 3
+
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid||||101,3 ng/µl||August 5
|-
|-
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||received from Edinburgh||||||August 3
+
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid||||95,7 ng/µl||August 5
|-
|-
-
|{{kulpart|BBa_I742142}}||pSB1A2||Sam8||received from Edinburgh||||||August 3
+
|{{kulpart|BBa_I742142}}||pSB1A2||Sam8||purified plasmid||||81,5 ng/µl||August 5
|-
|-
-
|{{kulpart|BBa_I742107}}||pSB1A2||ComT||received from Edinburgh||||||August 3
+
|{{kulpart|BBa_I742107}}||pSB1A2||ComT||purified plasmid||||122,9 ng/µl||August 5
|}
|}

Revision as of 09:01, 5 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
pSB1A2GFP + rbs + terminatorpurifiedjuly 29
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter regionrestricted with EcoRIjuly 29
/BLR promoter region4 microcentrifuge tubesjuly 30

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid101,3 ng/µlAugust 5
pSB1A2Sam8 + rbspurified plasmid95,7 ng/µlAugust 5
pSB1A2Sam8purified plasmid81,5 ng/µlAugust 5
pSB1A2ComTpurified plasmid122,9 ng/µlAugust 5

vanillin receptor

part plasmid description status μl left concentration date

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMJuly 24
pSB1A2medium constitutieve promoterJuly 23
ampicilline
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