http://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&feed=atom&action=historyTeam:KULeuven/Lab/Key/Lock/AntiKey - Revision history2024-03-29T12:05:32ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=42262&oldid=prevK3n: /* Key/Lock/AntiKey */2009-08-10T11:51:39Z<p><span class="autocomment">Key/Lock/AntiKey</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Goal==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Goal==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We want to test the affinity of the <del class="diffchange diffchange-inline">key </del>for the <del class="diffchange diffchange-inline">lock, </del>and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the <del class="diffchange diffchange-inline">antikey on </del>the level of transcription.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We want to test the affinity of the <ins class="diffchange diffchange-inline">Key </ins>for the <ins class="diffchange diffchange-inline">Lock </ins>and measure the amount of input signals needed to activate transcription. On the other hand<ins class="diffchange diffchange-inline">, </ins>we want to test the effect of adding the <ins class="diffchange diffchange-inline">Antikey at </ins>the level of transcription.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>First we will <del class="diffchange diffchange-inline">make </del>the test devices for the <del class="diffchange diffchange-inline">key </del>and <del class="diffchange diffchange-inline">lock</del>. Both Key and Lock <del class="diffchange diffchange-inline">will be </del>made constitutively. If <del class="diffchange diffchange-inline">we see </del>GFP, we <del class="diffchange diffchange-inline">will </del>know the <del class="diffchange diffchange-inline">key is working</del>. <del class="diffchange diffchange-inline">We don’t </del>need <del class="diffchange diffchange-inline">to test the system much rigorously</del>, since last year’s team already did this.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>First<ins class="diffchange diffchange-inline">, </ins>we will <ins class="diffchange diffchange-inline">prepare </ins>the test devices for the <ins class="diffchange diffchange-inline">Key </ins>and <ins class="diffchange diffchange-inline">Lock</ins>. Both Key and Lock <ins class="diffchange diffchange-inline">are </ins>made constitutively. If GFP <ins class="diffchange diffchange-inline">is present</ins>, we know the <ins class="diffchange diffchange-inline">Key works</ins>. <ins class="diffchange diffchange-inline">The system doesn't </ins>need <ins class="diffchange diffchange-inline">more rigorous testing</ins>, since last year’s team already did this.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>If we <del class="diffchange diffchange-inline">than also </del>add the <del class="diffchange diffchange-inline">antikey </del>device, we can measure how the rate of transcription lowers, depending on the amount of <del class="diffchange diffchange-inline">Tetracycline </del>added.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>If we <ins class="diffchange diffchange-inline">thereupon </ins>add the <ins class="diffchange diffchange-inline">Antikey </ins>device, we can measure how the rate of transcription lowers, depending on the amount of <ins class="diffchange diffchange-inline">tetracycline </ins>added.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">required</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">Required</ins>==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">biobricks</del>: </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">Biobricks</ins>: </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Key device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Key device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23066}} + {{kulpart|BBa_B0015}}: <del class="diffchange diffchange-inline">De </del>Key sequence with terminator is ordered at GeneArt. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23066}} + {{kulpart|BBa_B0015}}: <ins class="diffchange diffchange-inline">The </ins>Key sequence with terminator is ordered at GeneArt. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23110}}: <del class="diffchange diffchange-inline">promotor</del>, plasmid present in the lab.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23110}}: <ins class="diffchange diffchange-inline">Promotor</ins>, plasmid present in the lab.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Restriction enzymes EcoRI, XbaI and SpeI</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Restriction enzymes EcoRI, XbaI and SpeI</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#K12 E. Coli strains ( No special needs)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#K12 E. Coli strains ( No special needs)</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Lock device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Lock device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23110}}: <del class="diffchange diffchange-inline">promotor</del>, plasmid present in the lab</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23110}}: <ins class="diffchange diffchange-inline">Promotor</ins>, plasmid present in the lab</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23078}}: Lock Sequence is ordered at GeneArt</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#{{kulpart|BBa_J23078}}: Lock Sequence is ordered at GeneArt</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#{{kulpart|BBa_K145015}} + {{kulpart|BBa_B0015}}: GFP + LVA part present in the lab with terminator attached</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#{{kulpart|BBa_K145015}} + {{kulpart|BBa_B0015}}: GFP + LVA part present in the lab with terminator attached</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the AntiKey device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the AntiKey device</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#{{kulpart|BBa_K145201}}: TetR promotor, present in the lab</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#{{kulpart|BBa_K145201}}: TetR promotor, present in the lab</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#{{kulpart|BBa_K238005}} + B0015: <del class="diffchange diffchange-inline">ordered </del>at GeneArt</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#{{kulpart|BBa_K238005}} + B0015: <ins class="diffchange diffchange-inline">Ordered </ins>at GeneArt</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>• First all plasmids still present in the lab need to be isolated, so they’re ready to use. Then the GeneArt sequence of the Key can be ligated in the {{kulpart|BBa_J23110}} promotor plasmid.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>• First<ins class="diffchange diffchange-inline">, </ins>all plasmids still present in the lab need to be isolated, so they’re ready to use. Then the GeneArt sequence of the Key can be ligated in the {{kulpart|BBa_J23110}} promotor plasmid.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>• Next the {{kulpart|BBa_J23110}} <del class="diffchange diffchange-inline">Promotor </del>can be ligated to the GeneArt Lock sequence. If that’s done, the {{kulpart|BBa_K145015}}+{{kulpart|BBa_B0015}} construct can be ligated to the promotor/<del class="diffchange diffchange-inline">lock </del>sequence.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>• Next<ins class="diffchange diffchange-inline">, </ins>the {{kulpart|BBa_J23110}} <ins class="diffchange diffchange-inline">promotor </ins>can be ligated to the GeneArt Lock sequence. If that’s done, the {{kulpart|BBa_K145015}}+{{kulpart|BBa_B0015}} construct can be ligated to the promotor/<ins class="diffchange diffchange-inline">Lock </ins>sequence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>• These constructed plasmids can then be electroporated into the K12 E. coli cells and a GFP assay can be done<del class="diffchange diffchange-inline">, checking </del>if the Key/Lock system works properly. At this <del class="diffchange diffchange-inline">time</del>, we also need to measure the intensity or rate of fluorescence, in order to later <del class="diffchange diffchange-inline">check </del>if the Antikey works.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>• These constructed plasmids can then be electroporated into the K12 E. coli cells and a GFP assay can be done <ins class="diffchange diffchange-inline">to check </ins>if the Key/Lock system works properly. At this <ins class="diffchange diffchange-inline">moment</ins>, we also need to measure the intensity or rate of fluorescence, in order to <ins class="diffchange diffchange-inline">verify </ins>later if the Antikey works.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• At the same time the {{kulpart|BBa_K145201}} promotor can be ligated to the AntiKey GeneArt sequence, making the Antikey device.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• At the same time the {{kulpart|BBa_K145201}} promotor can be ligated to the AntiKey GeneArt sequence, making the Antikey device.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>II. Make sure the plates you grow the bacteria on contain the right antibiotic.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>II. Make sure the plates you grow the bacteria on contain the right antibiotic.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>III. You should always be able to identify not only the transformed colonies, but more importantly the recombinant colonies. This can be done by putting all three vectors in the cell or by combining <del class="diffchange diffchange-inline">to </del>devices on one plasmid.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>III. You should always be able to identify not only the transformed colonies, but more importantly the recombinant colonies. This can be done by putting all three vectors in the cell or by combining <ins class="diffchange diffchange-inline">two </ins>devices on one plasmid.</div></td></tr>
</table>K3nhttp://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=28261&oldid=prevMarianC.: /* Important */2009-07-27T13:12:31Z<p><span class="autocomment">Important</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>II. Make sure the plates you grow the bacteria on contain the right antibiotic.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>II. Make sure the plates you grow the bacteria on contain the right antibiotic.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>III. You should always be able to identify not only the transformed colonies, but more importantly the recombinant colonies.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>III. You should always be able to identify not only the transformed colonies, but more importantly the recombinant colonies<ins class="diffchange diffchange-inline">. This can be done by putting all three vectors in the cell or by combining to devices on one plasmid</ins>.</div></td></tr>
</table>MarianC.http://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27916&oldid=prevDeepstar: /* Steps */2009-07-27T07:25:12Z<p><span class="autocomment">Steps</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>• First all plasmids still present in the lab need to be isolated, so they’re ready to use. Then the GeneArt sequence of the Key can be ligated in the <del class="diffchange diffchange-inline">J23110 </del>promotor plasmid.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>• First all plasmids still present in the lab need to be isolated, so they’re ready to use. Then the GeneArt sequence of the Key can be ligated in the <ins class="diffchange diffchange-inline">{{kulpart|BBa_J23110}} </ins>promotor plasmid.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>• Next the <del class="diffchange diffchange-inline">J23110 </del>Promotor can be ligated to the GeneArt Lock sequence. If that’s done, the <del class="diffchange diffchange-inline">K145015</del>+<del class="diffchange diffchange-inline">B0015 </del>construct can be ligated to the promotor/lock sequence.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>• Next the <ins class="diffchange diffchange-inline">{{kulpart|BBa_J23110}} </ins>Promotor can be ligated to the GeneArt Lock sequence. If that’s done, the <ins class="diffchange diffchange-inline">{{kulpart|BBa_K145015}}</ins>+<ins class="diffchange diffchange-inline">{{kulpart|BBa_B0015}} </ins>construct can be ligated to the promotor/lock sequence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• These constructed plasmids can then be electroporated into the K12 E. coli cells and a GFP assay can be done, checking if the Key/Lock system works properly. At this time, we also need to measure the intensity or rate of fluorescence, in order to later check if the Antikey works.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• These constructed plasmids can then be electroporated into the K12 E. coli cells and a GFP assay can be done, checking if the Key/Lock system works properly. At this time, we also need to measure the intensity or rate of fluorescence, in order to later check if the Antikey works.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>• At the same time the <del class="diffchange diffchange-inline">K145201 </del>promotor can be ligated to the AntiKey GeneArt sequence, making the Antikey device.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>• At the same time the <ins class="diffchange diffchange-inline">{{kulpart|BBa_K145201}} </ins>promotor can be ligated to the AntiKey GeneArt sequence, making the Antikey device.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• In order to test the Antikey, all 3 plasmids need to be electroporated into the E.coli strains. Important is that all 3 plasmids contain a different control system (LacZ, Ampicillin or Kanamycin), so we can be sure the strains contain all three plasmids.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• In order to test the Antikey, all 3 plasmids need to be electroporated into the E.coli strains. Important is that all 3 plasmids contain a different control system (LacZ, Ampicillin or Kanamycin), so we can be sure the strains contain all three plasmids.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• By comparing the GFP results with and without Antikey, we can investigate whether our Antikey has an effect on the transcription of GFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>• By comparing the GFP results with and without Antikey, we can investigate whether our Antikey has an effect on the transcription of GFP.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Important==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Important==</div></td></tr>
</table>Deepstarhttp://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27915&oldid=prevDeepstar: /* required */2009-07-27T07:24:23Z<p><span class="autocomment">required</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 07:24, 27 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 23:</td>
<td colspan="2" class="diff-lineno">Line 23:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Key device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Key device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">J23066 </del>+ <del class="diffchange diffchange-inline">B0015</del>: De Key sequence with terminator is ordered at GeneArt. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">{{kulpart|BBa_J23066}} </ins>+ <ins class="diffchange diffchange-inline">{{kulpart|BBa_B0015}}</ins>: De Key sequence with terminator is ordered at GeneArt. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">J23110</del>: promotor, plasmid present in the lab.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">{{kulpart|BBa_J23110}}</ins>: promotor, plasmid present in the lab.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Restriction enzymes EcoRI, XbaI and SpeI</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Restriction enzymes EcoRI, XbaI and SpeI</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#K12 E. Coli strains ( No special needs)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#K12 E. Coli strains ( No special needs)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Lock device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Lock device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">J23110</del>: promotor, plasmid present in the lab</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">{{kulpart|BBa_J23110}}</ins>: promotor, plasmid present in the lab</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">J23078</del>: Lock Sequence is ordered at GeneArt</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">{{kulpart|BBa_J23078}}</ins>: Lock Sequence is ordered at GeneArt</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">K145015 </del>+ <del class="diffchange diffchange-inline">B0015</del>: GFP + LVA part present in the lab with terminator attached</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">{{kulpart|BBa_K145015}} </ins>+ <ins class="diffchange diffchange-inline">{{kulpart|BBa_B0015}}</ins>: GFP + LVA part present in the lab with terminator attached</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the AntiKey device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the AntiKey device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">K145201</del>: TetR promotor, present in the lab</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">{{kulpart|BBa_K145201}}</ins>: TetR promotor, present in the lab</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">K238005 </del>+ B0015: ordered at GeneArt</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">{{kulpart|BBa_K238005}} </ins>+ B0015: ordered at GeneArt</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td></tr>
</table>Deepstarhttp://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27913&oldid=prevDeepstar at 07:22, 27 July 20092009-07-27T07:22:18Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 07:22, 27 July 2009</td>
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<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Team:KULeuven/Common/BeginHeader}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Team:KULeuven/Common/SubMenu_Project}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Team:KULeuven/Common/EndHeader}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Key/Lock/AntiKey=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Key/Lock/AntiKey=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Deepstarhttp://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27669&oldid=prevMarianC.: /* Key/Lock/AntiKey */2009-07-25T12:14:55Z<p><span class="autocomment">Key/Lock/AntiKey</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:14, 25 July 2009</td>
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<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Goal==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Goal==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:endgoals.jpg|center|thumb|600px|End Goals for the systems]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:endgoals.jpg|center|thumb|600px|End Goals for the systems]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:testdevices.jpg|center|thumb|600px|How we will test if the system works]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:testdevices.jpg|center|thumb|600px|How we will test if the system works]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>First we will make the test devices for the key and lock. Both Key and Lock will be made constitutively. If we see GFP, we will know the key is working. We don’t need to test the system much rigorously, since last year’s team already did this.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>First we will make the test devices for the key and lock. Both Key and Lock will be made constitutively. If we see GFP, we will know the key is working. We don’t need to test the system much rigorously, since last year’s team already did this.</div></td></tr>
</table>MarianC.http://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27668&oldid=prevMarianC.: /* Key/Lock/AntiKey */2009-07-25T12:14:19Z<p><span class="autocomment">Key/Lock/AntiKey</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:14, 25 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 4:</td>
<td colspan="2" class="diff-lineno">Line 4:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:endgoals.jpg|center|thumb|<del class="diffchange diffchange-inline">500px</del>|End Goals for the systems]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:endgoals.jpg|center|thumb|<ins class="diffchange diffchange-inline">600px</ins>|End Goals for the systems]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:testdevices.jpg|center|thumb|<del class="diffchange diffchange-inline">365px</del>|How we will test if the system works]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:testdevices.jpg|center|thumb|<ins class="diffchange diffchange-inline">600px</ins>|How we will test if the system works]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>First we will make the test devices for the key and lock. Both Key and Lock will be made constitutively. If we see GFP, we will know the key is working. We don’t need to test the system much rigorously, since last year’s team already did this.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>First we will make the test devices for the key and lock. Both Key and Lock will be made constitutively. If we see GFP, we will know the key is working. We don’t need to test the system much rigorously, since last year’s team already did this.</div></td></tr>
</table>MarianC.http://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27667&oldid=prevMarianC.: /* Key/Lock/AntiKey */2009-07-25T12:14:02Z<p><span class="autocomment">Key/Lock/AntiKey</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:14, 25 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 4:</td>
<td colspan="2" class="diff-lineno">Line 4:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:endgoals.jpg|center|thumb|<del class="diffchange diffchange-inline">365px</del>|End Goals for the systems]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:endgoals.jpg|center|thumb|<ins class="diffchange diffchange-inline">500px</ins>|End Goals for the systems]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:testdevices.jpg|center|thumb|365px|How we will test if the system works]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:testdevices.jpg|center|thumb|365px|How we will test if the system works]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>MarianC.http://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27666&oldid=prevMarianC.: /* Key/Lock/AntiKey */2009-07-25T12:13:40Z<p><span class="autocomment">Key/Lock/AntiKey</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:13, 25 July 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 3:</td>
<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Goal==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Goal==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We want to test the affinity of the key for the lock, and measure the amount of input signals needed to activate transcription. On the other hand we want to test the effect of adding the antikey on the level of transcription.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:endgoals.jpg|center|thumb|365px|End Goals for the systems]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:testdevices.jpg|center|thumb|365px|How we will test if the system works]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>First we will make the test devices for the key and lock. Both Key and Lock will be made constitutively. If we see GFP, we will know the key is working. We don’t need to test the system much rigorously, since last year’s team already did this.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>First we will make the test devices for the key and lock. Both Key and Lock will be made constitutively. If we see GFP, we will know the key is working. We don’t need to test the system much rigorously, since last year’s team already did this.</div></td></tr>
</table>MarianC.http://2009.igem.org/wiki/index.php?title=Team:KULeuven/Lab/Key/Lock/AntiKey&diff=27664&oldid=prevMarianC.: /* required */2009-07-25T12:09:44Z<p><span class="autocomment">required</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:09, 25 July 2009</td>
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<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==required==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==required==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*biobricks: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*biobricks: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Key device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Key device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>J23066 + B0015: De Key sequence with terminator is ordered at GeneArt. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>J23066 + B0015: De Key sequence with terminator is ordered at GeneArt. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>J23110: promotor, plasmid present in the lab.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>J23110: promotor, plasmid present in the lab.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>Restriction enzymes EcoRI, XbaI and SpeI</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>Restriction enzymes EcoRI, XbaI and SpeI</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>K12 E. Coli strains ( No special needs)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>K12 E. Coli strains ( No special needs)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Lock device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the Lock device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>J23110: promotor, plasmid present in the lab</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>J23110: promotor, plasmid present in the lab</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>J23078: Lock Sequence is ordered at GeneArt</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>J23078: Lock Sequence is ordered at GeneArt</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>K145015 + B0015: GFP + LVA part present in the lab with terminator attached</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>K145015 + B0015: GFP + LVA part present in the lab with terminator attached</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the AntiKey device</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the AntiKey device</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>K145201: TetR promotor, present in the lab</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>K145201: TetR promotor, present in the lab</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">- </del>K238005 + B0015: ordered at GeneArt</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#</ins>K238005 + B0015: ordered at GeneArt</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Steps==</div></td></tr>
</table>MarianC.