• Main Components
• Design
• Modeling
• Wetlab Planning
• Notebook

Blue light Sensor

Biological Model

Mathematical Model

The entire network of interactions is visualized in the diagram below

Regulation of the production of mRNA key by incident blue light

Dimerization of YcgF

"Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [1]

Sensor interface

"Light Induced Structural Changes of a Full-length Protein and Its BLUF Domain in YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)" [2]

"Influence of a Joining Helix on the BLUF Domain of the YcgF Photoreceptor from Escherichia coli" [3]

YcgF - YcgE interaction

"The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli" [4]

Transcription of the RIBOKEY

https://2008.igem.org/Team:KULeuven/Model/Filter

Degradation of the RIBOKEY

https://2008.igem.org/Team:KULeuven/Model/Filter

References

[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"

[2] Y. Nakasone et al., "Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", Journal of the American Chemical Society, Apr. 2006