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Team:KULeuven/Modelling
From 2009.igem.org
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=Blue Light Sensor= | =Blue Light Sensor= | ||
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| + | ==Overview== | ||
The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light. | The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light. | ||
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[[Image:blue_light_sensor.jpg|center|thumb|630px|Regulation of the production of mRNA key by incident blue light]] | [[Image:blue_light_sensor.jpg|center|thumb|630px|Regulation of the production of mRNA key by incident blue light]] | ||
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| + | ==Reactions== | ||
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| + | ===Dimerization of YcgF=== | ||
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| + | "Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [http://pubs.acs.org/doi/full/10.1021/ja065682q] | ||
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| + | ===YcgF - YcgE interaction=== | ||
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| + | ===Transcription of the RIBOKEY=== | ||
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| + | ===Degradation of the RIBOKEY=== | ||
=References= | =References= | ||
Revision as of 13:47, 16 July 2009
Contents |
Blue Light Sensor
Overview
The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.
Input: Blue light (<math>\lambda = 470</math>)
Output: mRNA_RIBOKEY
The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of interacting with the operator-bound YcgE and to release it from the DNA.
The entire network of interactions is visualized in the diagram below
Reactions
Dimerization of YcgF
"Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [1]
YcgF - YcgE interaction
Transcription of the RIBOKEY
Degradation of the RIBOKEY
References
[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"
