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[[Image:YcgFdimerization.gif|center|thumb|450px|Sensor interface]] | [[Image:YcgFdimerization.gif|center|thumb|450px|Sensor interface]] | ||
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| + | "Light Induced Structural Changes of a Full-length Protein and Its BLUF Domain in | ||
| + | YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)" | ||
| + | [http://pubs.acs.org/doi/abs/10.1021/bi051820x] | ||
===YcgF - YcgE interaction=== | ===YcgF - YcgE interaction=== | ||
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===Degradation of the RIBOKEY=== | ===Degradation of the RIBOKEY=== | ||
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=References= | =References= | ||
Revision as of 20:07, 16 July 2009
Contents |
Blue Light Sensor
Overview
The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.
Input: Blue light (<math>\lambda = 470</math>)
Output: mRNA_RIBOKEY
The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of interacting with the operator-bound YcgE and to release it from the DNA.
The entire network of interactions is visualized in the diagram below
Reactions
Dimerization of YcgF
"Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [1]
"Light Induced Structural Changes of a Full-length Protein and Its BLUF Domain in YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)" [2]
YcgF - YcgE interaction
"The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli" [3]
Transcription of the RIBOKEY
https://2008.igem.org/Team:KULeuven/Model/Filter
Degradation of the RIBOKEY
https://2008.igem.org/Team:KULeuven/Model/Filter
References
[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"
