Team:KULeuven/Modelling

From 2009.igem.org

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=Blue Light Sensor=
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[https://2009.igem.org/Team:KULeuven/Blue_light_Sensor Blue light Sensor]
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[https://2009.igem.org/Team:KULeuven/Comperator Comperator]
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[https://2009.igem.org/Team:KULeuven/Vanillin_Sensor Vanillin Sensor]
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[https://2009.igem.org/Team:KULeuven/Vanillin_Production Vanillin Production]
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==Overview==
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=Overview=
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The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.
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Input: Blue light (<math>\lambda = 470</math>) <br>
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Output: mRNA_RIBOKEY
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[[Image:black_box_light_sensor.JPG|center|thumb|300px|Sensor interface]]
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The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation.
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The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of
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interacting with the operator-bound YcgE and to release it from the DNA.
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The entire network of interactions is visualized in the diagram below
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[[Image:blue_light_sensor.jpg|center|thumb|630px|Regulation of the production of mRNA key by incident blue light]]
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==Reactions==
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===Dimerization of YcgF===
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"Transient Dimerization and Conformational Change of a BLUF Protein:  YcgF", [http://pubs.acs.org/doi/full/10.1021/ja065682q]
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[[Image:YcgFdimerization.gif|center|thumb|450px|Sensor interface]]
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"Light Induced Structural Changes of a Full-length Protein and Its BLUF Domain in
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YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)"
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[http://pubs.acs.org/doi/abs/10.1021/bi051820x]
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===YcgF - YcgE interaction===
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"The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"
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[http://genesdev.cshlp.org/content/23/4/522.full.pdf+html]
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===Transcription of the RIBOKEY===
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https://2008.igem.org/Team:KULeuven/Model/Filter
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===Degradation of the RIBOKEY===
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https://2008.igem.org/Team:KULeuven/Model/Filter
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=References=
=References=

Revision as of 09:04, 17 July 2009

Blue light Sensor Comperator Vanillin Sensor Vanillin Production

Overview

References

[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"

Retrieved from "http://2009.igem.org/Team:KULeuven/Modelling"