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Team:KULeuven/Notebook/Key/Lock/AntiKey
From 2009.igem.org
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= Week 1: 6 July 2009 - 12 July 2009 = | = Week 1: 6 July 2009 - 12 July 2009 = | ||
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= Week 5: 3 August 2009 - 9 August 2009 = | = Week 5: 3 August 2009 - 9 August 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/3_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/3_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/4_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/4_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/5_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/5_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/6_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/6_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/7_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/7_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/8_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/8_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/9_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/9_August_2009/KeyLockAntiLock}} --> |
= Week 6: 10 August 2009 - 16 August 2009 = | = Week 6: 10 August 2009 - 16 August 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/10_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/10_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/11_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/11_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/12_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/12_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/13_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/13_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/14_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/14_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/15_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/15_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/16_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/16_August_2009/KeyLockAntiLock}} --> |
= Week 7: 17 August 2009 - 23 August 2009 = | = Week 7: 17 August 2009 - 23 August 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/17_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/17_August_2009/KeyLockAntiLock}} --> |
| - | <!--{{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/18_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/18_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/19_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/19_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/20_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/20_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/21_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/21_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/22_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/22_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/23_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/23_August_2009/KeyLockAntiLock}} --> |
= Week 8: 24 August 2009 - 30 August 2009 = | = Week 8: 24 August 2009 - 30 August 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/24_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/24_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/25_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/25_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/26_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/26_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/27_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/27_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/28_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/28_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/29_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/29_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/30_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/30_August_2009/KeyLockAntiLock}} --> |
= Week 9: 31 August 2009 - 6 September 2009 = | = Week 9: 31 August 2009 - 6 September 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/31_August_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/31_August_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/1_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/1_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/2_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/2_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/3_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/3_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/4_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/4_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/5_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/5_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/6_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/6_September_2009/KeyLockAntiLock}} --> |
= Week 10: 7 September 2009 - 13 September 2009 = | = Week 10: 7 September 2009 - 13 September 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/7_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/7_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/8_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/8_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/9_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/9_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/10_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/10_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/11_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/11_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/12_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/12_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/13_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/13_September_2009/KeyLockAntiLock}} --> |
= Week 11: 14 September 2009 - 20 September 2009 = | = Week 11: 14 September 2009 - 20 September 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/14_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/14_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/15_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/15_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/16_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/16_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/17_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/17_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/18_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/18_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/19_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/19_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/20_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/20_September_2009/KeyLockAntiLock}} --> |
= Week 12: 21 September 2009 - 27 September 2009 = | = Week 12: 21 September 2009 - 27 September 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/21_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/21_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/22_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/22_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/23_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/23_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/24_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/24_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/25_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/25_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/26_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/26_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/27_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/27_September_2009/KeyLockAntiLock}} --> |
= Week 13: 28 September 2009 - 4 October 2009 = | = Week 13: 28 September 2009 - 4 October 2009 = | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/28_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Monday|Team:KULeuven/28_September_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/29_September_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Tuesday|Team:KULeuven/29_September_2009/KeyLockAntiLock}} --> |
{{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/30_September_2009/KeyLockAntiLock}} | {{Team:KULeuven/EditableTemplateH3|Wednesday|Team:KULeuven/30_September_2009/KeyLockAntiLock}} | ||
{{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/1_October_2009/KeyLockAntiLock}} | {{Team:KULeuven/EditableTemplateH3|Thursday|Team:KULeuven/1_October_2009/KeyLockAntiLock}} | ||
{{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/2_October_2009/KeyLockAntiLock}} | {{Team:KULeuven/EditableTemplateH3|Friday|Team:KULeuven/2_October_2009/KeyLockAntiLock}} | ||
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/3_October_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Saturday|Team:KULeuven/3_October_2009/KeyLockAntiLock}} --> |
| - | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/4_October_2009/KeyLockAntiLock}} | + | <!-- {{Team:KULeuven/EditableTemplateH3|Sunday|Team:KULeuven/4_October_2009/KeyLockAntiLock}} --> |
= Week 14: 5 October 2009 - 11 October 2009 = | = Week 14: 5 October 2009 - 11 October 2009 = | ||
Revision as of 19:04, 17 October 2009
Week 1: 6 July 2009 - 12 July 2009
Week 2: 13 July 2009 - 19 July 2009
[edit] Thursday
Plating the glycerol stock strain containing .
Week 3: 20 July 2009 - 26 July 2009
[edit] Wednesday
Liquid culture of has been made.
[edit] Thursday
The plasmid of has been isolated from the liquid culture.
The glycerol stock strain containing has been plated.
[edit] Friday
The glycerol stock strain containing has been plated.
The key+terminator sequence, the lock sequence and the antikey+terminator sequence have all been ordered at GeneArt.
Week 4: 27 July 2009 - 2 August 2009
[edit] Monday
Following parts were put in liquid medium:
- + : GFP with LVA tag and terminators
- TetR-promoter
[edit] Tuesday
Following plasmids were purified:
- GFP with LVA tag
- concentration: 94,1 ng/μl
- 260/280: 1,94
- tetR-promoter
- concentration: 98,2 ng/μl
- 260/280: 1,89
[edit] Wednesday
[edit] Thursday
[edit] Friday
Week 5: 3 August 2009 - 9 August 2009
Week 6: 10 August 2009 - 16 August 2009
Week 7: 17 August 2009 - 23 August 2009
Week 8: 24 August 2009 - 30 August 2009
Week 9: 31 August 2009 - 6 September 2009
Week 10: 7 September 2009 - 13 September 2009
Week 11: 14 September 2009 - 20 September 2009
Week 12: 21 September 2009 - 27 September 2009
Week 13: 28 September 2009 - 4 October 2009
[edit] Wednesday
- Liquid culture of the two components has been made
[edit] Thursday
- Miniprepped and nanodropped
| sample | ng/µl |
|---|---|
| L1 | 93,5 |
| L2 | 81,1 |
| AK1 | 75,3 |
| AK2 | 72,8 |
- Restriction digest overnight: enzymes EcoRI and PstI
[edit] Friday
- Gel electrophoresis and purification
- Ligation of lock and antisense key in pSB1A2 (over weekend)
Week 14: 5 October 2009 - 11 October 2009
[edit] Monday
- Electroporation of the ligation
- Plating out the electroporated cells
[edit] Tuesday
- No growth visible
[edit] Wednesday
- Therefore new attempt to ligate the two components in iGEM vectors
- Restriction digest: EcoRI and PstI
[edit] Thursday
- Gel electrophoresis and purification
- Ligation (overnight): both lock and antisense key are ligated in pSB1A2 and pSB3K3
[edit] Friday
- Electroporated and plated out the ligations
[edit] Saturday
[edit] Sunday
- Liquid culture (the lock and antisense key in iGEM vectors)
Week 15: 12 October 2009 - 18 October 2009
[edit] Monday
Miniprep and nanodrop
- Lock (pSB1A2): ng/µl
- Antisense key (pSB3K3): ng/µl
[edit] Tuesday
[edit] Wednesday
[edit] Thursday
[edit] Friday
[edit] Saturday
[edit] Sunday
Week 16: 19 October 2009 - 25 October 2009
[edit] Monday
[edit] Tuesday
[edit] Wednesday
[edit] Thursday
[edit] Friday
[edit] Saturday
[edit] Sunday
Week 17: 26 October 2009 - 1 November 2009
[edit] Monday
[edit] Tuesday
[edit] Wednesday
[edit] Thursday
[edit] Friday
[edit] Saturday
[edit] Sunday
Week 18: 2 November 2009 - 8 November 2009
[edit] Monday
[edit] Tuesday
[edit] Wednesday
[edit] Thursday
[edit] Friday
[edit] Saturday
[edit] Sunday
