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Team:KULeuven/Notebook/Key/Lock/AntiKey
From 2009.igem.org
Week 1: 6 July 2009 - 12 July 2009
Week 2: 13 July 2009 - 19 July 2009
[edit] Thursday
Plating the glycerol stock strain containing .
Week 3: 20 July 2009 - 26 July 2009
[edit] Wednesday
Liquid culture of has been made.
[edit] Thursday
The plasmid of has been isolated from the liquid culture.
The glycerol stock strain containing has been plated.
[edit] Friday
The glycerol stock strain containing has been plated.
The key+terminator sequence, the lock sequence and the antikey+terminator sequence have all been ordered at GeneArt.
Week 4: 27 July 2009 - 2 August 2009
[edit] Monday
Following parts were put in liquid medium:
- + : GFP with LVA tag and terminators
- TetR-promoter
[edit] Tuesday
Following plasmids were purified:
- GFP with LVA tag
- concentration: 94,1 ng/μl
- 260/280: 1,94
- tetR-promoter
- concentration: 98,2 ng/μl
- 260/280: 1,89
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Week 5: 3 August 2009 - 9 August 2009
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Week 6: 10 August 2009 - 16 August 2009
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Week 7: 17 August 2009 - 23 August 2009
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Week 8: 24 August 2009 - 30 August 2009
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