"
Team:KULeuven/Project/Details
From 2009.igem.org
(Difference between revisions)
(→Blue light receptor) |
Bart Bosmans (Talk | contribs) |
||
| Line 7: | Line 7: | ||
The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. | The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. | ||
| - | We designed the | + | We designed the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238002 BBa_K238002] part in such way that irradiation with a certain amount of blue light activates transcription of key-mRNA. To achieve this, we purified the promoter-region of E. coli MC4100. After mutating out possible restriction sites, the blr promot [http://partsregistry.org/wiki/index.php?title=Part:BBa_K238000 BBa_K238000] part was added to the registry. |
== Vanillin receptor == | == Vanillin receptor == | ||
Revision as of 11:59, 31 August 2009
Contents |
Blue light receptor
The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. We designed the BBa_K238002 part in such way that irradiation with a certain amount of blue light activates transcription of key-mRNA. To achieve this, we purified the promoter-region of E. coli MC4100. After mutating out possible restriction sites, the blr promot BBa_K238000 part was added to the registry.
Vanillin receptor
Vanillin production
Key antikey
