Team:KULeuven/Project/Details

From 2009.igem.org

Revision as of 09:50, 31 August 2009 by K3n (Talk | contribs)

Contents

Blue light receptor

Biologie blue light.png

The protein YcgF is a known blue-light sensor in certain E. coli strains. Upon photo-excitation it dimerizes and acts as an anti-repressor for YcgE. YcgE is bound to the promotor-region and inhibits RNA Polymerase. The dimerized YcgF interacts directly with the repressor, releasing it from the DNA and allowing transcription. We designed the K238002 part in such way that irradiation with a certain amount of blue light activates transcription of key-mRNA. To achieve this, we purified the promoter-region of E. coli MC4100. After mutating out possible restriction sites, the blr promot K238000 part was added to the registry.

Vanillin receptor

Biologie vanillin receptor.png

Vanillin production

Biologie vanillin synthesis.png
Vanillin production overview starting from Tyrosine.

Key antikey

Biologie blue light.png
Biologie antikey.png