Team:KULeuven/Project Results

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= Project Results =
= Project Results =
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Our Results can be divided in two groups, the parts we cut and pasted together to make (test)-devices and characterizations of a part.
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Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.
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- First and foremost we succesfully made the following parts:
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- First and foremost we succesfully assembled the following biobricks:
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'''Partnumber''' '''Description'''
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'''Partnumber'''     '''Description'''
{{kulpart|K238005}} Antisense key
{{kulpart|K238005}} Antisense key
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{{kulpart|K238029}} Fcs + rbs
{{kulpart|K238029}} Fcs + rbs
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All these parts were sent to the registry.  
All these parts were sent to the registry.  
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The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor and the effect of promotor strenght on blue light irradiance was examined.  
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The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor, furthermore the effect of promotor strenght on blue light irradiance was examined.  
{|
{|
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| [[Image:weakness.png|thumb|365px|Comparison of the blue light promotor (blue) to a standard promotor strength {{kulpart|I20260} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor]]
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| [[Image:weakness.png|thumb|400px|Comparison of the blue light promotor (blue) to a standard promotor strength {{kulpart|I20260} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor]]
|}
|}
{|
{|
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| [[Image:Light-dark.png|thumb|365px|Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)]]
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| [[Image:Light-dark.png|thumb|400px|Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)]]
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|}

Revision as of 14:48, 19 October 2009


Project Results

Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.

- First and foremost we succesfully assembled the following biobricks:

Partnumber Description

Antisense key

Sam5 + rbs

virA

VirG

VirB

Blue light promotor

Ech+fcs

Blp + GFP

Blp + RFP

EF + terminator

EF+ promoter+ terminator

Sam5 +sam8

Sams + promoter

Sams + promoter + comt

Sams+ comt+ promoter en terminator

lock

Sam8 + RBS

Ech + rbs

Fcs + rbs

All these parts were sent to the registry.


The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor, furthermore the effect of promotor strenght on blue light irradiance was examined.

I20260} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor
Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)



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