Team:KULeuven/Project Results

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Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.
Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.
-
- First and foremost we succesfully assembled the following biobricks:
+
First and foremost we succesfully assembled the following biobricks and send them to the registry:
-
'''Partnumber'''     '''Description'''
+
{| border ="0" align="Justify"
 +
!| Partnumber      ||Description
 +
|- align="center"
 +
|{{kulpart|K238005}} ||Antisense key
 +
|- align="center"
 +
|{{kulpart|K238007}} ||Sam5 + rbs
 +
|- align="center"
 +
|{{kulpart|K238008}} ||virA
 +
|- align="center"
 +
|{{kulpart|K238009}} ||VirG
 +
|- align="center"
 +
|{{kulpart|K238011}} ||VirB
 +
|- align="center"
 +
|{{kulpart|K238013}} ||Blue light promotor
 +
|- align="center"
 +
|{{kulpart|K238014}} ||Ech+fcs
 +
|- align="center"
 +
|{{kulpart|K238015}} ||Blp + GFP
 +
|- align="center"
 +
|{{kulpart|K238016}} ||Blp + RFP
 +
|- align="center"
 +
|{{kulpart|K238017}} ||EF + terminator
 +
|- align="center"
 +
|{{kulpart|K238018}} ||EF+ promoter+ terminator
 +
|- align="center"
 +
|{{kulpart|K238019}} ||Sam5 +sam8
 +
|- align="center"
 +
|{{kulpart|K238020}} ||Sams + promoter
 +
|- align="center"
 +
|{{kulpart|K238021}} ||Sams + promoter + comt
 +
|- align="center"
 +
|{{kulpart|K238022}} ||Sams+ comt+ promoter en terminator
 +
|- align="center"
 +
|{{kulpart|K238024}} ||lock
 +
|- align="center"
 +
|{{kulpart|K238027}} ||Sam8 + RBS
 +
|- align="center"
 +
|{{kulpart|K238028}} ||Ech + rbs
 +
|- align="center"
 +
|{{kulpart|K238029}} ||Fcs + rbs
 +
|}
-
{{kulpart|K238005}} Antisense key
 
-
{{kulpart|K238007}} Sam5 + rbs
+
Furthermore, the blue light promotor was extensively characterized. The strenght of the promotor was compared to a normal promotor and the effect of promotor strenght on blue light irradiance was examined.  
-
 
+
-
{{kulpart|K238008}} virA
+
-
 
+
-
{{kulpart|K238009}} VirG
+
-
 
+
-
{{kulpart|K238011}} VirB
+
-
 
+
-
{{kulpart|K238013}} Blue light promotor
+
-
 
+
-
{{kulpart|K238014}} Ech+fcs
+
-
 
+
-
{{kulpart|K238015}} Blp + GFP
+
-
 
+
-
{{kulpart|K238016}} Blp + RFP
+
-
 
+
-
{{kulpart|K238017}} EF + terminator
+
-
 
+
-
{{kulpart|K238018}} EF+ promoter+ terminator
+
-
 
+
-
{{kulpart|K238019}} Sam5 +sam8
+
-
 
+
-
{{kulpart|K238020}} Sams + promoter
+
-
 
+
-
{{kulpart|K238021}} Sams + promoter + comt
+
-
 
+
-
{{kulpart|K238022}} Sams+ comt+ promoter en terminator
+
-
 
+
-
{{kulpart|K238024}} lock
+
-
 
+
-
{{kulpart|K238027}} Sam8 + RBS
+
-
 
+
-
{{kulpart|K238028}} Ech + rbs
+
-
 
+
-
{{kulpart|K238029}} Fcs + rbs
+
-
 
+
-
All these parts were sent to the registry.
+
-
 
+
-
 
+
-
The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor, furthermore the effect of promotor strenght on blue light irradiance was examined.  
+
{|
{|

Revision as of 15:00, 19 October 2009


Project Results

Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.

First and foremost we succesfully assembled the following biobricks and send them to the registry:

Partnumber Description
Antisense key
Sam5 + rbs
virA
VirG
VirB
Blue light promotor
Ech+fcs
Blp + GFP
Blp + RFP
EF + terminator
EF+ promoter+ terminator
Sam5 +sam8
Sams + promoter
Sams + promoter + comt
Sams+ comt+ promoter en terminator
lock
Sam8 + RBS
Ech + rbs
Fcs + rbs


Furthermore, the blue light promotor was extensively characterized. The strenght of the promotor was compared to a normal promotor and the effect of promotor strenght on blue light irradiance was examined.

Comparison of the blue light promotor (blue) to a standard promotor strength (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor
Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)



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