Team:KULeuven/Project Results

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Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.
Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.
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First and foremost we succesfully assembled the following biobricks and send them to the registry:
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First and foremost we succesfully assembled the following biobricks and sent them to the registry:
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Furthermore, the blue light promotor was extensively characterized. The strenght of the promotor was compared to a normal promotor and the effect of promotor strenght on blue light irradiance was examined.  
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Furthermore, the blue light promoter was extensively characterized. The strength of the promoter was compared to a normal promoter and the effect of promoter strength on blue light irradiance was examined.  
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{|
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| [[Image:weakness.png|thumb|400px|Comparison of the blue light promotor (blue) to a standard promotor strength {{kulpart|I20260}} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor]]
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| [[Image:weakness.png|thumb|400px|Comparison of the blue light promoter (blue) to a standard promoter strength {{kulpart|I20260}} (orange) in RPU. The blue light promoter shows at least half the strength of the standard promoter, meaning it can be classified as a weak promoter]]
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{|
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| [[Image:Light-dark.png|thumb|400px|Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)]]
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| [[Image:Light-dark.png|thumb|400px|Effect of blue light irradiance on promoter strength. Cells grown under blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)]]
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Revision as of 21:21, 19 October 2009


Project Results

Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.

First and foremost we succesfully assembled the following biobricks and sent them to the registry:

Partnumber Description
Antisense key
Sam5 + rbs
virA
VirG
VirB
Blue light promotor
Ech+fcs
Blp + GFP
Blp + RFP
EF + terminator
EF+ promoter+ terminator
Sam5 +sam8
Sams + promoter
Sams + promoter + comt
Sams+ comt+ promoter en terminator
lock
Sam8 + RBS
Ech + rbs
Fcs + rbs


Furthermore, the blue light promoter was extensively characterized. The strength of the promoter was compared to a normal promoter and the effect of promoter strength on blue light irradiance was examined.

Comparison of the blue light promoter (blue) to a standard promoter strength (orange) in RPU. The blue light promoter shows at least half the strength of the standard promoter, meaning it can be classified as a weak promoter
Effect of blue light irradiance on promoter strength. Cells grown under blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)



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