Team:KULeuven/Sandbox

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Marriage Between iGEM and KUL in 2009

Contents

File:Home page voorstel.pdf

Regulation bacteria

The main idea is to develop a bacteria that regulates the concentration of a molecule 'X' and keeps it constant by a dynamical equilibrium (see presentation). This is being done by simultaneous synthesis and degradation (with 1 or 2 being dependant on concentration), a dynamical equilibrium exists when the synthesis equals the degradation. This dynamical equilibrium can be set by using light sensor, the light intensity would then be proportional to the concentration for the equilibrium.

problems

Find magical molecule 'X'


Ideas for in aquarium (nutrients for fish):

Vitamine:

  • Vitamine B12
    • [http://ecocyc.org/ECOLI/substring-search?type=NIL&object=Vitamine+B12&quickSearch=Quick+Search Vitamine B12 on ecocyc.org]
  • Thiamin
    • [http://ecocyc.org/ECOLI/substring-search?type=NIL&object=thiamin&quickSearch=Quick+Search Thiamin on ecocyc.org] (biosynthesis + ABC transporter)
  • Choline
    • [http://ecocyc.org/ECOLI/substring-search?type=NIL&object=choline&quickSearch=Quick+Search Choline on ecyco.org] (metabolism + uptake)
  • vitamine K
    • [http://ecocyc.org/ECOLI/substring-search?type=NIL&object=Vitamine+K&quickSearch=Quick+Search Vitamine K on ecocyc.org]
    • But no export mechanism
      • TAT sequentie aanbouwen, systeem voor volledig gevouwen protein naar buiten brengen


Essentiele aminoacids:

  • Methionine
    • [http://ecocyc.org/ECOLI/substring-search?type=NIL&object=Methionine&quickSearch=Quick+Search Methionine on ecocyc.org]
    • But no export mechanism
      • TAT sequentie aanbouwen, systeem voor volledig gevouwen protein naar buiten brengen


Overige:

  • pH
  • O2

Andere ideeën:

  • LCD idee
    • polarisatie door L en R moleculen, een ervan afbreken wat de verhouding tussen de 2 veranderd
  • Geur bacterie
    • Vanille geur
    • biobricks
      • [http://partsregistry.org/Odor Odor bricks]
        • (Banana odor generator)
        • (Wintergreen odor generator)
        • (Feruloyl-CoA hydratase for vanillin biosynthesis protein generator) <-- nog geen experience/DNA in kit
        • (Synthetic periplasmic binding protein that docks a vanillin molecule)
      • Ervaring van [http://openwetware.org/wiki/IGEM:MIT/2006/Blurb iGEM 2006 groep] ivm wintergreen en banana odor generator
    • Meten van vanille


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