Team:KULeuven/Used Parts

From 2009.igem.org

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(→Individual parts)
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!| Partnumber||Short description|| Resistance || Status||Location||Additional notes
!| Partnumber||Short description|| Resistance || Status||Location||Additional notes
|-align="center"
|-align="center"
-
| {{kulpart|BBa_K238001|icon}}|| OmpF promotor + key || || Biobrick made || ||  
+
| {{kulpart|BBa_K238001|icon}}|| VirB promotor + key || || Biobrick in registry || ||  
|-align="center"
|-align="center"
-
| {{kulpart|BBa_K238002|icon}}|| Blr promotor + key || || Biobrick made || ||  
+
| {{kulpart|BBa_K238002|icon}}|| Blr promotor + key || || Biobrick in registry || ||  
|-align="center"
|-align="center"
-
| {{kulpart|BBa_K238003|icon}}|| Vanillin receptor || || Biobrick made || ||  
+
| {{kulpart|BBa_K238003|icon}}|| Vanillin receptor || || Biobrick in registry || ||  
|-align="center"
|-align="center"
-
| {{kulpart|BBa_K238004|icon}}|| Vanillin synthesis|| || Biobrick made || || 5 paths in pathway never tested together in E. Coli
+
| {{kulpart|BBa_K238004|icon}}|| Vanillin synthesis|| || Biobrick in registry || || 5 paths in pathway never tested together in E. Coli
|-align="center"
|-align="center"
-
| {{kulpart|BBa_K238006|icon}}|| Short Vanillin synthesis|| || Biobrick made || || Short version containing only the last 2 parts of the pathway, these have been tested in E. Coli
+
| {{kulpart|BBa_K238006|icon}}|| Short Vanillin synthesis|| || Biobrick in registry || || Short version containing the last 2 genes of the pathway, these have been tested in E. Coli
|-align="center"
|-align="center"
 +
| {{kulpart|BBa_K238012|icon}}|| Short Vanillin synthesis II|| || Biobrick in registry || || Short version containing the first 3 parts of the pathway, these have not been tested in E. Coli
[[Category:Team:KULeuven/BioBricks]]
[[Category:Team:KULeuven/BioBricks]]

Revision as of 08:36, 31 July 2009

Individual parts

PartnumberShort description Resistance StatusLocationAdditional notes
Ribokey A/A ordered at Geneart
antikey A/A ordered at geneart
lock Ap ordered at geneart
Terminator Ak Available here -80
RBS Ap Available here -80
promotor Ap Available here -80
Sam8 send from Edinburgh
Sam5 send from Edinburgh
comt A/A send from Edinburgh
fcs ap received from Edinburgh -20
ech ap received from Edinburgh -20
VirA receptor primers ordered This is purified from Agrobacterium temefaciens
VirG activator primers ordered This is purified from Agrobacterium tumefaciens
RpoA primers ordered This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
VirB promoter region primers ordered This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
YcgF/E promotor -20 region purified, actual promoter identified blue light promotor from [1]


Composite parts

PartnumberShort description Resistance StatusLocationAdditional notes
VirB promotor + key Biobrick in registry
Blr promotor + key Biobrick in registry
Vanillin receptor Biobrick in registry
Vanillin synthesis Biobrick in registry 5 paths in pathway never tested together in E. Coli
Short Vanillin synthesis Biobrick in registry Short version containing the last 2 genes of the pathway, these have been tested in E. Coli
Short Vanillin synthesis II Biobrick in registry Short version containing the first 3 parts of the pathway, these have not been tested in E. Coli
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