Team:KULeuven/Wetlab

From 2009.igem.org

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'''Vanillin Production'''
'''Vanillin Production'''
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Testing three enzyme (from Tyrosine to Ferrulic acid) and two enzyme pathway (from Ferrulic acid to Vanillin)and eventually combining the five enzymes.
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Testing the three enzyme (from Tyrosine to Ferrulic acid) and two enzyme pathway (from Ferrulic acid to Vanillin) and eventually combining the five enzymes.

Revision as of 09:56, 3 September 2009

The labwork is divided into four groups, each working on a specific aspect of Essencia coli


Blue Light Receptor

Purifying the promotor region and creating it into a new biobrick


Vanillin Production

Testing the three enzyme (from Tyrosine to Ferrulic acid) and two enzyme pathway (from Ferrulic acid to Vanillin) and eventually combining the five enzymes.


Vanillin Receptor

Removing unwanted restriction sites, sequencing and combining all elements into a standard iGEM plasmid.


Key/Lock/Antikey

Ligating Key, Lock and Antikey into promotor plasmids and testing the system with a GFP assay.