Team:KU Seoul/Details
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#PCR of BioBrick Parts | #PCR of BioBrick Parts | ||
- | + | General PCR condition : 94℃(3’)-[94℃(1’)-53℃(1’)-72℃(3’)]30-72℃(5’)-4℃ | |
{| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1" | {| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1" | ||
! PCR mixture | ! PCR mixture | ||
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|D.W. || align="center"| 35.5 | |D.W. || align="center"| 35.5 | ||
|} | |} | ||
- | + | #Hot start PCR condition : 95℃(15’)-[95℃(1’)-53℃(1’)-72℃(3’)]30-72℃(10’)-4℃ | |
{| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1" | {| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1" | ||
! PCR mixture | ! PCR mixture | ||
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|D.W. || align="center"| 25.5 | |D.W. || align="center"| 25.5 | ||
|} | |} | ||
- | # | + | #Result (Figure 2) |
[[Image:KU_Seoul_2.jpg]] | [[Image:KU_Seoul_2.jpg]] | ||
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Revision as of 07:05, 20 October 2009
The ExperimentsMaterials
Experiment Dairy
General PCR condition : 94℃(3’)-[94℃(1’)-53℃(1’)-72℃(3’)]30-72℃(5’)-4℃
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