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Team:KU Seoul/Materials
From 2009.igem.org
Revision as of 11:13, 21 October 2009 by
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Home
Team
Project
Overview
Materials
Experiment Diary
Result
Parts
Notebook
Safety
Sponsors
Materials
Backbone Plasmids
Plasmid pSB3C5 with
BBa_J04450
: 2009 Kit Plate 1 [5C]
Plasmid pSB3T5 with
BBa_J04450
: 2009 Kit Plate 1 [9C]
Promoters
Promoter arsR [ParsR], zntA [Pznt] and yodA [PyodA](ref) originated from genomic DNA of Escherichia coli XL-1 Blue
Protein Coding Sequences
Green fluorescent protein(BBa_E0044)
: 2009 Kit Plate 1 [14G]
Red fluorescent protein(BBa_E1010)
: 2009 Kit Plate 1 [18F]
Aryl acylamidase protein(ref) :
new biological part
Primers
Template
Primer
Sequence
Length(bp)
Plasmid pSB3C5
pSB3C5_F
gctgctgttTAATAAtactagtagcggccgctgc
34
pSB3C5(+Pars)_R
taataggtgtgaattttgagttggCtctagaagcggccgcga
42
Plasmid pSB3T5
pSB3T5_F
gacgaaaactacgctgctgctgttTAATAAtactagtagcggccgctgc
49
pSB3T5_R
GTCGGCAATATGAAGctctagaagcggccgcga
33
Promoter yodA
PyodA_F
cggccgcttctagagCTTCATATTGCCGACAAAGTACG
38
PyodA_R
ATGTGACTTACCCATaacAGTTTCCTCCAGAGTCATTG
38
Green fluorescent protein
GR(+Pars)_F
aattcacacctattaccttcctctgcacttacacattcgttaagtcatatATGc
78
gtaaaggagaagaacttttcactg
GR(+Pznt)_R
ctggagtcgactccagagtcaagttttatcagagatacagcgagcggacg
84
TCTAGTttattaaacagcagcagcgtagttttcg
Red fluorescent protein
RF(+Pznt)_F
tgataaaacttgactctggagtcgactccagagtgtatccttcggttaatATG
75
gcttcctccgaagacgttatca
RF(+AAV tag)_R
TTATTAaacagcagcagcgtagttttcgtcgtttgctgcAgcaccggtgg
59
agtgacgac
Aryl acylamidase
AMD_F
CTGGAGGAAACTGTTatgGGTAAGTCACATTCGCCAG
37
AMD(+AAV tag)_R
TTATTAaacagcagcagcgtagttttcgtcgtttgctgcCAGGGGC
55
CGTCCGGCG
Chemicals
Acetaminophen (Sigma)
CdCl2∙H2O (Junsei)
ZnCl2 (Sigma)
KH2AsO3 (Sigma)
Luria-Bertani broth & Bacto agar (Difco)
o-Cresol (Sigma)
CuSO4∙5H2O (Sigma)