Team:KU Seoul/Parts

From 2009.igem.org

(Difference between revisions)
(Part)
(Part)
 
(3 intermediate revisions not shown)
Line 18: Line 18:
=== Part ===
=== Part ===
-
::'''Mercury sensor - Mercury inducing promoter''' {{Part|BBa_K271000}} = Part:{{Part|BBa_I728456}} + amd gene
+
:'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}})
[[Image:amd_part.png]]
[[Image:amd_part.png]]
-
:Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations,  
+
:Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.  
-
:cells are able to adapt and resume growth after a period of stasis. Promoter yodA is only transiently activated  
+
 
-
:during cadmium-induced growth inhibition. Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the  
+
:Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.  
-
:amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing  
+
 
-
:a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having  
+
-
:amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-
+
-
:purple color, aryl acylamidase gene will apply to reporter gene.  
+
:'''Heavy-metal detector {{Part|BBa_K271001}}'''
:'''Heavy-metal detector {{Part|BBa_K271001}}'''
-
:The promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer  
+
:The promoter/operator region of the zntA gene of Escherichia coli. It shows that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat sequence.  
-
:of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the  
+
-
:promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat  
+
-
:sequence.  
+
::[[Image:zinc_part.png]]
::[[Image:zinc_part.png]]

Latest revision as of 15:51, 21 October 2009






Part

Mercury sensor - Mercury inducing promoter Part:BBa_I728456 + amd gene(BBa_K271000)

Amd part.png

Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.
Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.


Heavy-metal detector BBa_K271001
The promoter/operator region of the zntA gene of Escherichia coli. It shows that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat sequence.
Zinc part.png