Team:KU Seoul/Parts

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Part

Mercury sensor - Mercury inducing promoter : Part:BBa_I728456 + amd gene BBa_K271000

Amd part.png

Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations,
cells are able to adapt and resume growth after a period of stasis. Promoter yodA is only transiently activated
during cadmium-induced growth inhibition. Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the
amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing
a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having
amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-
purple color, aryl acylamidase gene will apply to reporter gene.
Heavy-metal detector BBa_K271001
The promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer
of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the
promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat
sequence.
Zinc part.png