Team:KU Seoul/Result

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Revision as of 09:41, 20 October 2009

Results

1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp

1)Pznt-rfp

Zn²⁺ (mM)	0	0.5	0.75	1	1.5	2
OD₆₀₀	0.490 ± 0.003	0.459 ± 0.004	0.433 ± 0.008	0.418 ± 0.005	0.275 ± 0.011	0.243 ± 0.008
Red fluorescence (RF)	26628 ± 706	27856 ± 292	29333 ± 292	31217 ± 66	46044 ± 237	49188 ± 174
OD₆₀₀/RF	54376 ± 1203	60646 ± 787	67803 ± 573	74687 ± 755	167833 ± 7202	202565 ± 6428
Background	0	6270 ± 520	13427 ± 1719	20311 ± 475	113457 ± 6016	148189 ± 6731

2)Pars-gfp

AsO^3- (mM)	0	0.00625	0.125	0.25	0.5	0.75	1
OD₆₀₀	0.553 ± 0.009	0.519 ± 0.002	0.480 ± 0.005	0.395 ± 0.007	0.315 ± 0.006	0.286 ± 0.004	0.273 ± 0.006
Green fluorescence (GF)	27740 ± 226	26411 ± 169	25267 ± 267	25536 ± 213	25407 ± 259	24849 ± 152	26479 ± 149
OD₆₀₀/GF	50206 ± 1225	50855 ± 123	52606 ± 689	64669 ± 1684	80665 ± 603	86897 ± 1321	96784 ± 2165
Background	0	648 ± 1314	2399 ± 969	14463 ± 2896	30459 ± 1631	36691 ± 1636	46578 ± 3220

2.Synthetic circuit – II : PyodA-AMD

Cd²⁺ (mM)	0	0.05	0.1	0.2	0.4	0.8
OD₆₁₅	0.242 ± 0.005	0.258 ± 0.008	0.274 ± 0.011	0.284 ± 0.01	0.293 ± 0.011	0.302 ± 0.01
Background	26628 ± 706	27856 ± 292	29333 ± 292	31217 ± 66	46044 ± 237	49188 ± 174

@@ Line 16: / Line 16: @@
 {|style=  "background:#FFCC33;" align="center"
 |
-:1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp
 == Results ==
+:1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp
+::1)Pznt-rfp
 {| style="color:Black; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"
-|align="center"|Zn2+ (mM) || align="center"| 0 || align="center"| 0.5 || align="center"| 0.75 || align="center"| 1 || align="center"| 1.5 || align="center"| 2
+|align="center"|Zn<Sup>2+</Sup> (mM)
+| align="center"| 0
+| align="center"| 0.5
+| align="center"| 0.75
+| align="center"| 1
+| align="center"| 1.5
+| align="center"| 2
 |-
-|align="center"|OD<sub>600</sub || align="center"| 0.490 ± 0.003 || align="center"| 0.459
+|align="center"|OD<sub>600</sub>
-± 0.004 || align="center"| 0.433
+| align="center"| 0.490 ± 0.003
-± 0.008 || align="center"| 0.418
+| align="center"| 0.459 ± 0.004
-± 0.005 || align="center"| 0.275
+| align="center"| 0.433 ± 0.008
-± 0.011 || align="center"| 0.243
+| align="center"| 0.418 ± 0.005
-± 0.008
+| align="center"| 0.275 ± 0.011
+| align="center"| 0.243 ± 0.008
 |-
-|align="center"|Red fluorescence (RF) || align="center"| 26628
+|align="center"|Red fluorescence (RF)
-± 706 || align="center"| 27856
+| align="center"| 26628 ± 706
-± 292 || align="center"| 29333
+| align="center"| 27856 ± 292
-± 292 || align="center"| 31217
+| align="center"| 29333 ± 292
-± 66 || align="center"| 46044
+| align="center"| 31217 ± 66
-± 237 || align="center"| 49188
+| align="center"| 46044 ± 237
-± 174
+| align="center"| 49188 ± 174
+|-
+|align="center"|OD<sub>600</sub>/RF
+| align="center"| 54376 ± 1203
+| align="center"| 60646 ± 787
+| align="center"| 67803 ± 573
+| align="center"| 74687 ± 755
+| align="center"| 167833 ± 7202
+| align="center"| 202565 ± 6428
+|-
+|align="center"|Background
+| align="center"| 0
+| align="center"| 6270 ± 520
+| align="center"| 13427 ± 1719
+| align="center"| 20311 ± 475
+| align="center"| 113457 ± 6016
+| align="center"| 148189 ± 6731
+|}
+[[Image:KU Seoul Result RFP.jpeg]]
+::2)Pars-gfp
+{| style="color:Black; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"
+|align="center"|AsO<Sup>3-</Sup> (mM)
+| align="center"| 0
+| align="center"| 0.00625
+| align="center"| 0.125
+| align="center"| 0.25
+| align="center"| 0.5
+| align="center"| 0.75
+| align="center"| 1
+|-
+|align="center"|OD<sub>600</sub>
+| align="center"| 0.553 ± 0.009
+| align="center"| 0.519 ± 0.002
+| align="center"| 0.480 ± 0.005
+| align="center"| 0.395 ± 0.007
+| align="center"| 0.315 ± 0.006
+| align="center"| 0.286 ± 0.004
+| align="center"| 0.273 ± 0.006
+|-
+|align="center"|Green fluorescence (GF)
+| align="center"| 27740 ± 226
+| align="center"| 26411 ± 169
+| align="center"| 25267 ± 267
+| align="center"| 25536 ± 213
+| align="center"| 25407 ± 259
+| align="center"| 24849 ± 152
+| align="center"| 26479 ± 149
+|-
+|align="center"|OD<sub>600</sub>/GF
+| align="center"| 50206 ± 1225
+| align="center"| 50855 ± 123
+| align="center"| 52606 ± 689
+| align="center"| 64669 ± 1684
+| align="center"| 80665 ± 603
+| align="center"| 86897 ± 1321
+| align="center"| 96784 ± 2165
+|-
+|align="center"|Background
+| align="center"| 0
+| align="center"| 648 ± 1314
+| align="center"| 2399 ± 969
+| align="center"| 14463 ± 2896
+| align="center"| 30459 ± 1631
+| align="center"| 36691 ± 1636
+| align="center"| 46578 ± 3220
+|}
+[[Image:KU Seoul Result GFP.jpeg]]
+[[Image:KU Seoul Calibrationcurve.jpeg]]
+:2.Synthetic circuit – II : PyodA-AMD
+{| style="color:Black; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"
+|align="center"|Cd<Sup>2+</Sup> (mM)
+| align="center"| 0
+| align="center"| 0.05
+| align="center"| 0.1
+| align="center"| 0.2
+| align="center"| 0.4
+| align="center"| 0.8
 |-
-|align="center"|OD<sub>600</sub/RF || align="center"| 54376
+|align="center"|OD<sub>615</sub>
-± 1203 || align="center"| 60646
+| align="center"| 0.242 ± 0.005
-± 787 || align="center"| 67803
+| align="center"| 0.258 ± 0.008
-± 573 || align="center"| 74687
+| align="center"| 0.274 ± 0.011
-± 755 || align="center"| 167833
+| align="center"| 0.284 ± 0.01
-± 7202 || align="center"| 202565
+| align="center"| 0.293 ± 0.011
-± 6428
+| align="center"| 0.302 ± 0.01
 |-
-|align="center"|Background || align="center"| 6270
+|align="center"|Background
-± 520 || align="center"| 13427
+| align="center"| 26628 ± 706
-± 1719 || align="center"| 20311
+| align="center"| 27856 ± 292
-± 475 || align="center"| 113457
+| align="center"| 29333 ± 292
-± 6016 || align="center"| 1.5 || align="center"| 148189
+| align="center"| 31217 ± 66
-± 6731
+| align="center"| 46044 ± 237
+| align="center"| 49188 ± 174
 |}
-(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
-Your abstract
 |}