"
Team:Kyoto/Calendar/24 July 2009
From 2009.igem.org
(Difference between revisions)
ShoTakamori (Talk | contribs) (New page: 090724 Image:090724iGEMnote.ppt Initial Training Takashima) |
(形式変更) |
||
| (14 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | + | __NOTOC__ | |
| + | {{:Team:Kyoto/CSS}} | ||
| + | {{:Team:Kyoto/Template:Leftmenu}} | ||
| + | {{:Team:Kyoto/CSS/notebook}} | ||
| + | <html><div id="maincontents"></html> | ||
| + | <!-- EDIT below [ここより下を編集してください] --> | ||
| + | {{:Team:Kyoto/Calendar}} | ||
| + | |||
| + | ==To Do on 24 July== | ||
| + | *Miniprep (3-O-2, 3-A-4) | ||
| + | *Restriction Enzyme Digestion | ||
| + | *Electrophoresis | ||
| + | |||
| + | ==Results== | ||
| + | ===Miniprep=== | ||
| + | *3-O-2, 3-A-4 was minipreped. | ||
| + | |||
| + | ===Digestion=== | ||
| + | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel. | ||
| + | |||
| + | {| border="1" style="text-align:center;width:50%" | ||
| + | |+Sample list | ||
| + | !NO.!!Sample Name!!Enzyme | ||
| + | |-align="center" | ||
| + | |1||3-O-2||Eco R1, PST1 | ||
| + | |-align="center" | ||
| + | |2||3-A-4||Eco R1, PST1 | ||
| + | |-align="center" | ||
| + | |3||3-O-2||Eco R1 | ||
| + | |-align="center" | ||
| + | |4||3-A-4||Eco R1 | ||
| + | |} | ||
| + | |||
| + | ===Electrophoresis=== | ||
| + | [[Image:Kyoto_e_090724_1.png|250px|right]] | ||
| + | * Very clear bands were found. (See the gel image in right!) | ||
| + | * The bands are listed below. | ||
| + | |||
| + | {| border="1" style="text-align:center;width:50%" | ||
| + | |+Parts and Plasmid length | ||
| + | !!!3-O-2!!3-A-4 | ||
| + | |- | ||
| + | |Parts length / bp||2837||487 | ||
| + | |- | ||
| + | |Plasmid length / bp||3266||3266 | ||
| + | |} | ||
| + | <!-- EDIT above [ここより上を編集してください] --> | ||
| + | <html> | ||
| + | </div><!--end of maincontents--> | ||
| + | </html> | ||
Latest revision as of 06:53, 14 August 2009
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
