Team:Kyoto/Calendar/24 July 2009

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=='''Lab-note on 24 July 2009'''==
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{| class="notebook"
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==To Do on 24 July==
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|Experimenter||'''Kashima, Takashima, Hibino, Miyazawa'''
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*Miniprep (3-O-2, 3-A-4)
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|-
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*Restriction Enzyme Digestion
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|Note-taker||'''Takashima'''
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*Electrophoresis
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====Title====
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==Results==
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{| class="notebook"
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===Miniprep===
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|'''7/24 Miniprep Kit, Restriction Enzyme Digestion Electrophoresis'''
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*3-O-2, 3-A-4 was minipreped.
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===Digestion===
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*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
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{| border="1" style="text-align:center;width:50%"
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|+Sample list
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!NO.!!Sample Name!!Enzyme
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|-align="center"
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|1||3-O-2||Eco R1, PST1
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|-align="center"
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|2||3-A-4||Eco R1, PST1
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|-align="center"
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|3||3-O-2||Eco R1
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|-align="center"
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|4||3-A-4||Eco R1
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|}
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====Main Protocol====
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===Electrophoresis===
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{| class="notebook"
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[[Image:Kyoto_e_090724_1.png|250px|right]]
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|
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* Very clear bands were found. (See the gel image in right!)
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#Miniprep Kit
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* The bands are listed below.
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#Restriction Enzyme Digestion
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#Electrophoresis
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{| border="1" style="text-align:center;width:50%"
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|}
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|+Parts and Plasmid length
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====Storage list====
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!!!3-O-2!!3-A-4
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{| class="notebook"
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!Sample Date!!Sample Name!!Enzyme!!purification Kit!!Product!!Storage
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|-
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|iGEM DNA parts kit||3-O-2||Eco R1|| || ||6F freezer
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|-
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|iGEM DNA parts kit||3-A-4||Eco R1|| || ||6F freezer
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|-
|-
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|iGEM DNA parts kit||3-O-2||Eco R1<br>PST1|| || ||6F freezer
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|Parts length / bp||2837||487
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|-
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|iGEM DNA parts kit||3-A-4||Eco R1<br>PST1|| || ||6F freezer
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|Plasmid length / bp||3266||3266
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|}
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====Miniprep====
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{| class="notebook"
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|
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'''Protocol'''
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*[3-O-2, 3-A-4]
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#Cultured samples (090722 9:30 ～ 090723 16:50)
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#Centrifuged 2mins at 15000g 4℃.
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#Removed the top of layer.
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#Centrifuged 2mins at 15000g 4℃.
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#Removed the top of layer.
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#Centrifuged 10mins at 14000g 4℃.
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#Applied  the top of layer to column.
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#Centrifuged RT, 10sec and remove through.
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#Added Buffer PB 500µl.
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#Centrifuged RT, 10sec and remove through.
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#Added Buffer PE 650µl.
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#Centrifuged RT, 10sec and remove through.
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#Centrifuged 1min at 17900g 4℃.
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#Exchanged tube. (remove Ethanol)
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#Added Milli Q 50µl RT 1min.
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#Centrifuged 1min at 17900g 4℃.
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#Collected through and measured concentration.
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----
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'''Results'''
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{| class="data"
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!Sample!!conc./(ng/µl)
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|-
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|3-O-2||283.5
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|-
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|3-A-4||103.9
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|}
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|}
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====Electrophoresis====
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{| class="notebook"
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|
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'''Protocol'''
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*Gel:'''Agarose'''
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*Time:'''12:50-13:20'''
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*Voltage:'''100 V'''
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{| class="data"
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! !!1!!2!!3!!4
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|-
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!Sample
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|3-O-2 (3 µl)||3-A-4 (3 µl)||3-O-2 (3 µl)||3-A-4 (3 µl)
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|-
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!enzyme
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|Eco R1 (0.17 µl)||Eco R1 (0.17 µl)||Eco R1 (0.17 µl)||Eco R1 (0.17 µl)
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|-
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!enzyme
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|PST1 (0.3 µl)||PST1 (0.3 µl)|| ||
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|-
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!Water / µl
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|1 ||1 ||1.4||1.4
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|-
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!H buffer / µl
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|8.2||8.2||8.1||8.1
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|-
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!Total / µl
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!12.7!!12.7!!12.9!!12.9
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|}
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Incubated at 37 ℃ (090724 12:50 to 13:20 )
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{| class="data"
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! !!1!!2!!3!!4!!5
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|-
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!Sample
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|3-O-2||3-A-4||3-O-2||3-A-4||Marker
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|-
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!volume / µl
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|10||10||13||13||2.5
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|-
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!6 times loddingbuffer / µl
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|2||2||2.6||2.6||0.5
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|}
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----
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'''Results'''
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[[Image:Kyoto_e_090724_1.png|thumb|300px|Result of Electrophoresis.]]
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{| class="data"
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! !!3-O-2!!3-A-4
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|-
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!Parts length / bp
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|2837||487
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|-
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!Plasmid length / bp
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|3266||3266
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|}
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----
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'''Discussion'''
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This result indicates that the parts ( 3-O-2, 3-A-4) were  treated with  enzyme in appropriate way.
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|}
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Latest revision as of 06:53, 14 August 2009

March
MTWTFSS
            [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_March_2009&action=edit 1]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_March_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_March_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_March_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_March_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_March_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_March_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_March_2009&action=edit 8]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_March_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_March_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_March_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_March_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_March_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_March_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_March_2009&action=edit 15]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_March_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_March_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_March_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_March_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_March_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_March_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_March_2009&action=edit 22]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_March_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/24_March_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_March_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_March_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_March_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_March_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_March_2009&action=edit 29]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_March_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/31_March_2009&action=edit 31]
April
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_April_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_April_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_April_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_April_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_April_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_April_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_April_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_April_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_April_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_April_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_April_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_April_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_April_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_April_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_April_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_April_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_April_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_April_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_April_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_April_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_April_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_April_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_April_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/24_April_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_April_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_April_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_April_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_April_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_April_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_April_2009&action=edit 30]
May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_May_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_May_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_May_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_May_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_May_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_May_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_May_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_May_2009&action=edit 17]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_May_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_May_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_May_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_May_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_May_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_May_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/24_May_2009&action=edit 24]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_May_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_May_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_May_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_May_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_May_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_May_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_June_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_June_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_June_2009&action=edit 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_July_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_July_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_July_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_July_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_July_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_July_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_July_2009&action=edit 23] [http://2009.igem.org/Team:Kyoto/Calendar/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_August_2009&action=edit 2]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_August_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_August_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_August_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_August_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_August_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_August_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_August_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_August_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_August_2009&action=edit 16]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_August_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_August_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_August_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/24_August_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_August_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_August_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/31_August_2009&action=edit 31]
September
MTWTFSS
  [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_September_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_September_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_September_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_September_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_September_2009&action=edit 6]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_September_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_September_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_September_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_September_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_September_2009&action=edit 13]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_September_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_September_2009&action=edit 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/1_October_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/2_October_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/4_October_2009&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/5_October_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/6_October_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/7_October_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/8_October_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/9_October_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/11_October_2009&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/12_October_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/13_October_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/14_October_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/15_October_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/16_October_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/17_October_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/18_October_2009&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/19_October_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/20_October_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/21_October_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Kyoto/Calendar/31_October_2009&action=edit 31]

To Do on 24 July

  • Miniprep (3-O-2, 3-A-4)
  • Restriction Enzyme Digestion
  • Electrophoresis

Results

Miniprep

  • 3-O-2, 3-A-4 was minipreped.

Digestion

  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1

Electrophoresis

Kyoto e 090724 1.png
  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

Retrieved from "http://2009.igem.org/Team:Kyoto/Calendar/24_July_2009"