Team:Kyoto/CiC/Notebook/1019-1023

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(Difference between revisions)
(0914-0918 : what to do. what to do. what to do. what to do.)
(0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator)
Line 81: Line 81:
**Colony PCR
**Colony PCR
**Insert check
**Insert check
 +
 +
{{:Team:Kyoto/Pad_start_dates}}
 +
===Monday, 14 August===
 +
====To Do====
 +
*p-2-(1)(4)(7)(8)(10)
 +
**Miniprep
 +
**Restriction Enzyme Digestion
 +
*p-2-(1)(4)  E.X
 +
**PCR purification kit
 +
*p-2-(1)(7)(8)(10)  E.S
 +
**Gel extraction
 +
*4-(1)(2)(3)
 +
**Colony PCR
 +
**Insert check
 +
 +
====Results====
 +
=====p-2-(1)(4)(7)(8)(10)=====
 +
=====p-2-(1)(7)(8)(10)  E.S=====
 +
*Gel extraction kit
 +
[[Image:Kyoto_0914_PCR.png|250px]]
 +
{| class="table"
 +
|+Miniprep product conc.
 +
!NO.!!Sample name!!conc./(ng/ul)
 +
|-
 +
|1||p-2-(1)||9.3
 +
|-
 +
|2||p-2-(7)||25.9
 +
|-
 +
|3||p-2-(8)||8.6
 +
|-
 +
|4||p-2-(10)||7.5
 +
|}
 +
 +
=====4-(1)(2)(3)=====
 +
[[Image:Kyoto_0914_PCR_2.png|250px]]
 +
*This result indicates that (1)a, (2)b and (3)a were inserted properly.
 +
 +
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}

Revision as of 09:31, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator


Monday, 14 August

To Do

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check

Results

p-2-(1)(4)(7)(8)(10)
p-2-(1)(7)(8)(10) E.S
  • Gel extraction kit

Kyoto 0914 PCR.png

Miniprep product conc.
NO.Sample nameconc./(ng/ul)
1p-2-(1)9.3
2p-2-(7)25.9
3p-2-(8)8.6
4p-2-(10)7.5
4-(1)(2)(3)

Kyoto 0914 PCR 2.png

  • This result indicates that (1)a, (2)b and (3)a were inserted properly.



Tuesday, 15 September

To Do

Further to 14 Sept.

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check


Monday, 14 August

To Do

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check

Results

p-2-(1)(4)(7)(8)(10)
p-2-(1)(7)(8)(10) E.S
  • Gel extraction kit

Kyoto 0914 PCR.png

Miniprep product conc.
NO.Sample nameconc./(ng/ul)
1p-2-(1)9.3
2p-2-(7)25.9
3p-2-(8)8.6
4p-2-(10)7.5
4-(1)(2)(3)

Kyoto 0914 PCR 2.png

  • This result indicates that (1)a, (2)b and (3)a were inserted properly.





Wednesday, 16 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)