Team:Kyoto/CiC/Notebook/1019-1023

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(→0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator)
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-
==0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator ==
+
==1019-1025 : what to do. what to do. what to do. what to do. ==
 +
 
{{:Team:Kyoto/Pad_start_dates}}
{{:Team:Kyoto/Pad_start_dates}}
-
===Monday, 14 August===
+
===Monday, 19 October===
====To Do====
====To Do====
-
*p-2-(1)(4)(7)(8)(10)
 
-
**Miniprep
 
-
**Restriction Enzyme Digestion
 
-
*p-2-(1)(4)  E.X
 
-
**PCR purification kit
 
-
*p-2-(1)(7)(8)(10)  E.S
 
-
**Gel extraction
 
-
*4-(1)(2)(3)
 
-
**Colony PCR
 
-
**Insert check
 
-
 
====Results====
====Results====
-
=====p-2-(1)(4)(7)(8)(10)=====
+
{{:Team:Kyoto/Pad_between_dates}}
-
=====p-2-(1)(7)(8)(10)  E.S=====
+
-
*Gel extraction kit
+
-
[[Image:Kyoto_0914_PCR.png|250px]]
+
-
{| class="table"
+
-
|+Miniprep product conc.
+
-
!NO.!!Sample name!!conc./(ng/ul)
+
-
|-
+
-
|1||p-2-(1)||9.3
+
-
|-
+
-
|2||p-2-(7)||25.9
+
-
|-
+
-
|3||p-2-(8)||8.6
+
-
|-
+
-
|4||p-2-(10)||7.5
+
-
|}
+
-
=====4-(1)(2)(3)=====
 
-
[[Image:Kyoto_0914_PCR_2.png|250px]]
 
-
*This result indicates that (1)a, (2)b and (3)a were inserted properly.
 
 +
===Tuesday, 20 October===
 +
====To Do====
 +
====Results====
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
-
===Tuesday, 15 September===
+
===Wednesday, 21 October===
====To Do====
====To Do====
-
'''Further to 14 Sept.'''
+
====Results====
 +
{{:Team:Kyoto/Pad_between_dates}}
-
*p-2-(1)(4)(7)(8)(10)
+
===Thursday, 22 October===
-
**Miniprep
+
-
**Restriction Enzyme Digestion
+
-
*p-2-(1)(4)  E.X
+
-
**PCR purification kit
+
-
*p-2-(1)(7)(8)(10)  E.S
+
-
**Gel extraction
+
-
*4-(1)(2)(3)
+
-
**Colony PCR
+
-
**Insert check
+
-
 
+
-
{{:Team:Kyoto/Pad_start_dates}}
+
-
===Monday, 14 August===
+
====To Do====
====To Do====
-
*p-2-(1)(4)(7)(8)(10)
 
-
**Miniprep
 
-
**Restriction Enzyme Digestion
 
-
*p-2-(1)(4)  E.X
 
-
**PCR purification kit
 
-
*p-2-(1)(7)(8)(10)  E.S
 
-
**Gel extraction
 
-
*4-(1)(2)(3)
 
-
**Colony PCR
 
-
**Insert check
 
-
 
====Results====
====Results====
-
=====p-2-(1)(4)(7)(8)(10)=====
 
-
=====p-2-(1)(7)(8)(10)  E.S=====
 
-
*Gel extraction kit
 
-
[[Image:Kyoto_0914_PCR.png|250px]]
 
-
{| class="table"
 
-
|+Miniprep product conc.
 
-
!NO.!!Sample name!!conc./(ng/ul)
 
-
|-
 
-
|1||p-2-(1)||9.3
 
-
|-
 
-
|2||p-2-(7)||25.9
 
-
|-
 
-
|3||p-2-(8)||8.6
 
-
|-
 
-
|4||p-2-(10)||7.5
 
-
|}
 
-
 
-
=====4-(1)(2)(3)=====
 
-
[[Image:Kyoto_0914_PCR_2.png|250px]]
 
-
*This result indicates that (1)a, (2)b and (3)a were inserted properly.
 
-
 
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
-
{{:Team:Kyoto/Pad_between_dates}}
+
===Friday, 23 October===
-
===Wednesday, 16 September===
+
====To Do====
====To Do====
-
**ligation
+
====Results====
-
**transformation
+
{{:Team:Kyoto/Pad_between_dates}}
 +
===Saturday, 24 October===
 +
====To Do====
====Results====
====Results====
-
=====ligation=====
+
{{:Team:Kyoto/Pad_between_dates}}
-
{| class="table"
+
-
|+conc.
+
-
!NO.!!Sample name!!conc./(ng/ul)
+
-
|-
+
-
|1||11(stu1)||103.2
+
-
|}
+
-
 
+
-
=====Transformation=====
+
-
No colony was observed (090917 10:00)　
+
 +
===Saturday, 25 October===
 +
====To Do====
 +
====Results====
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
-
===Friday, 11 September===
+
===Sunday, 26 October===
====To Do====
====To Do====
-
*Make repetitive sequence (MPR)
+
====Results====
-
*Transformation(retry)
+
 
{{:Team:Kyoto/Pad_endof_dates}}
{{:Team:Kyoto/Pad_endof_dates}}
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Revision as of 13:21, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1019-1025 : what to do. what to do. what to do. what to do.

Monday, 19 October

To Do

Results


Tuesday, 20 October

To Do

Results

Wednesday, 21 October

To Do

Results

Thursday, 22 October

To Do

Results

Friday, 23 October

To Do

Results

Saturday, 24 October

To Do

Results

Saturday, 25 October

To Do

Results

Sunday, 26 October

To Do

Results

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/1019-1023"