Team:Kyoto/CiC/Notebook/1019-1023

From 2009.igem.org

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==1019-1025 : Obserbation ==
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==1019-1025 :Obserbation ==
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===Monday, 19 October===
===Monday, 19 October===
====To Do====
====To Do====
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====Results====
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* subgoal A --- Confirmation of function of TAT-liposome
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**observation by microscope
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* Subgoal B --- Liposomes with Mitochondrial Translocases
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**Mitochondoria isolation
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**expressing sig-GFP in vitro with mitochondoria
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* Subgoal C --- sig-EGFP expression in HeLa
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**transfomation
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===Tuesday, 20 October===
 
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====To Do====
 
====Results====
====Results====
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=====subgoal A --- Confirmation of function of TAT-liposome=====
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===Wednesday, 21 October===
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Liposome was taken by HeLa cells.See the [[Team:Kyoto/CiC/Results|results]].
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====To Do====
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====Results====
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===Thursday, 22 October===
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=====Subgoal C --- sig-EGFP expression in HeLa=====
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====To Do====
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sig-EGFP was made a localization in mitochondoria.
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====Results====
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See the [[Team:Kyoto/CiC/Results|results]].
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===Friday, 23 October===
 
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====To Do====
 
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====Results====
 
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===Saturday, 24 October===
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===Tuesday, 20 October===
====To Do====
====To Do====
 +
* Subgoal B --- Liposomes with Mitochondrial Translocases
 +
**observation with microscope
====Results====
====Results====
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See the [[Team:Kyoto/CiC/Results|results]].
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===Saturday, 25 October===
 
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====To Do====
 
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====Results====
 
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===Sunday, 26 October===
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===Wednesday, 21 October===
====To Do====
====To Do====
 +
* subgoal A --- Confirmation of function of TAT-liposome
 +
**making liposome by natural swelling method and extruder
 +
**add liposome to HeLa cells
====Results====
====Results====
 +
According to the expectation,HIV-TAT-(LALAAAA)3-generator may work.
 +
Liposome is taken by HeLa Cells.
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See the [[Team:Kyoto/CiC/Results|results]].
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Latest revision as of 03:08, 22 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1019-1025 :Obserbation


Monday, 19 October

To Do

  • subgoal A --- Confirmation of function of TAT-liposome
    • observation by microscope
  • Subgoal B --- Liposomes with Mitochondrial Translocases
    • Mitochondoria isolation
    • expressing sig-GFP in vitro with mitochondoria
  • Subgoal C --- sig-EGFP expression in HeLa
    • transfomation

Results

subgoal A --- Confirmation of function of TAT-liposome

Liposome was taken by HeLa cells.See the results.

Subgoal C --- sig-EGFP expression in HeLa

sig-EGFP was made a localization in mitochondoria. See the results.



Tuesday, 20 October

To Do

  • Subgoal B --- Liposomes with Mitochondrial Translocases
    • observation with microscope

Results

See the results.



Wednesday, 21 October

To Do

  • subgoal A --- Confirmation of function of TAT-liposome
    • making liposome by natural swelling method and extruder
    • add liposome to HeLa cells

Results

According to the expectation,HIV-TAT-(LALAAAA)3-generator may work. Liposome is taken by HeLa Cells. See the results.