"
Team:Kyoto/CiC/Notebook/1019-1023
From 2009.igem.org
(Difference between revisions)
(→subgoal A Confirmation of function of TAT-liposome) |
(→Results) |
||
| (3 intermediate revisions not shown) | |||
| Line 44: | Line 44: | ||
====Results==== | ====Results==== | ||
=====subgoal A --- Confirmation of function of TAT-liposome===== | =====subgoal A --- Confirmation of function of TAT-liposome===== | ||
| - | + | Liposome was taken by HeLa cells.See the [[Team:Kyoto/CiC/Results|results]]. | |
| - | |||
| - | |||
| - | |||
=====Subgoal C --- sig-EGFP expression in HeLa===== | =====Subgoal C --- sig-EGFP expression in HeLa===== | ||
sig-EGFP was made a localization in mitochondoria. | sig-EGFP was made a localization in mitochondoria. | ||
| Line 57: | Line 54: | ||
===Tuesday, 20 October=== | ===Tuesday, 20 October=== | ||
====To Do==== | ====To Do==== | ||
| + | * Subgoal B --- Liposomes with Mitochondrial Translocases | ||
| + | **observation with microscope | ||
====Results==== | ====Results==== | ||
| + | See the [[Team:Kyoto/CiC/Results|results]]. | ||
| + | |||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
| + | |||
===Wednesday, 21 October=== | ===Wednesday, 21 October=== | ||
====To Do==== | ====To Do==== | ||
| + | * subgoal A --- Confirmation of function of TAT-liposome | ||
| + | **making liposome by natural swelling method and extruder | ||
| + | **add liposome to HeLa cells | ||
====Results==== | ====Results==== | ||
| + | According to the expectation,HIV-TAT-(LALAAAA)3-generator may work. | ||
| + | Liposome is taken by HeLa Cells. | ||
| + | See the [[Team:Kyoto/CiC/Results|results]]. | ||
{{:Team:Kyoto/Pad_endof_dates}} | {{:Team:Kyoto/Pad_endof_dates}} | ||
<html></div><!-- /#rightcontents --></html> | <html></div><!-- /#rightcontents --></html> | ||
Latest revision as of 03:08, 22 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1019-1025 :Obserbation
Monday, 19 October
To Do
- subgoal A --- Confirmation of function of TAT-liposome
- observation by microscope
- Subgoal B --- Liposomes with Mitochondrial Translocases
- Mitochondoria isolation
- expressing sig-GFP in vitro with mitochondoria
- Subgoal C --- sig-EGFP expression in HeLa
- transfomation
Results
subgoal A --- Confirmation of function of TAT-liposome
Liposome was taken by HeLa cells.See the results.
Subgoal C --- sig-EGFP expression in HeLa
sig-EGFP was made a localization in mitochondoria. See the results.
Tuesday, 20 October
To Do
- Subgoal B --- Liposomes with Mitochondrial Translocases
- observation with microscope
Results
See the results.
Wednesday, 21 October
To Do
- subgoal A --- Confirmation of function of TAT-liposome
- making liposome by natural swelling method and extruder
- add liposome to HeLa cells
Results
According to the expectation,HIV-TAT-(LALAAAA)3-generator may work. Liposome is taken by HeLa Cells. See the results.
