Team:Kyoto/GSDD/Notebook/0810-0816

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GSDD
Notebook

0727-0802: Construction.
0803-0809: Construction.
0810-0816: Construction.
0817-0823: Construction.PCR.
0824-0830: Construction.PCR.
0831-0906: Construction.PCR.MPR.
0907-0913: Construction.PCR.MPR.
0914-0920: Construction.PCR.MPR.
0921-0927: Construction.PCR.MPR.
0928-1004: Construction.PCR.MPR.
1005-1011: Construction.PCR.MPR.
1012-1018: Construction.MPR.Observation.

0810-0816 : Construction.

Monday, 10 August - Friday, 14 August

To Do

Transformation
- 1-N-1
- 1-K-14
- 2-N-13

Results

colony

1-N-1: observed
1-K-14: observed
2-N-13(1): none
2-N-13(2): none
2-N-13(3): none

We didn’t check that 2-N-13 was Kanamycin resistant at first(1,2). We made LB plate(+Kan), and tried the transformation again(3). However, no colony was observed. PCR amplification is required for amplifying 2-N-13.