Team:LCG-UNAM-Mexico/Project

From 2009.igem.org

(Difference between revisions)
(Prototype team page)
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{|align="justify"
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__NOTOC__
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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<html>
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|[[Image:Example_logo.png|200px|right]]
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|-
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right]]
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|-
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|align="center"|[[Team:LCG-UNAM-Mexico | Team Example 2]]
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|}
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<!--- The Mission, Experiments --->
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<head>
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!align="center"|[[Team:LCG-UNAM-Mexico|Home]]
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!align="center"|[[Team:LCG-UNAM-Mexico/Team|The Team]]
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var mime;
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!align="center"|[[Team:LCG-UNAM-Mexico/Project|The Project]]
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!align="center"|[[Team:LCG-UNAM-Mexico/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:LCG-UNAM-Mexico/Modeling|Modeling]]
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!align="center"|[[Team:LCG-UNAM-Mexico/Notebook|Notebook]]
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|}
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== '''Overall project''' ==
 
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Your abstract
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                <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico" onMouseOver="window.status= 'Click here to return Home'; return true;">Home
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                <!--[if gt IE 6]><!--></a><!--<![endif]-->
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                <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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              <li>
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                <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Record">Record
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              <li>
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                <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Project">Project</a>
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                <ul class="DropDownMenu" id="Ddm1">
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  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Parts"><span><span>Parts</span></span></a></li>
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                  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Resources"><span><span>Resources</span></span></a></li>
 +
                  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Description</span>>></span></a>
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                      <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Design</span></span></a></li>
 +
  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Delivery</span></span></a></li>
 +
  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Defense</span></span></a></li>
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              <li>
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                <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team">Team</a>
 +
 
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<ul class="DropDownMenu" id="Ddm2">
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                  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team"><span><span>Aknowledgments</span></span></a></li>
 +
                  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team"><span><span>Collaborations</span></span></a></li>
 +
                  <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team"><span><span>Sponsors</span></span></a></li>
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{|
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|-valign="top" border="0"
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|width="900px" style="padding: 0 20px 0 0;"|
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=='''The Project'''==
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Bacteriophage infection represents a common matter in science and industry. We propose to contend with such infections at a population level by triggering a defense system delivered by an engineered version of the satellite phage P4. This phage will be produced into an E. coli strain which harbors some genes from the helper phage P2 that are used for complementing and completing P4 life cycle, hence creating a production line of our version of P4.
 +
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This will enable phage production and subsequent delivery of the defense system into wild-type E. coli and possibly other bacterial host strains.
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The defense will consist of DNA and RNA degradation by toxins which will be activated
 +
by T3 or T7 RNA-Polymerases and transcribed fast enough to stop phage assembly and scattering in the environment.
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 +
Moreover, the system includes the spread of a quorum sensing signal, hence "warning" the population to prepare against further T3 or T7 infection.
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We will implement a stochastic population model to simulate the infection processes and  quantify the efficiency of our system.
 +
|}
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== Project Details==
 
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=== Part 2 ===
 
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<html>
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<header>
 +
<script language="JavaScript">
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=== The Experiments ===
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<style>
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#bodyContent { padding: 10px auto; width: 1000px; margin: auto; clear: none; }
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a:link { color: #659D32; text-decoration: none}
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a:visited { color:#659D32; text-decoration: none}
 +
a:hover { color:#f29400; text-decoration: none}
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a:active { color:#f29400; text-decoration: none}
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</style>
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=== Part 3 ===
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<body>
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<table border="0" align="center" width="900" cellpadding="50"><tr><th><a href="#" onCLick="window.history.go(-1); return false;"><img border="0"  src="https://static.igem.org/mediawiki/2009/d/d5/Flecha_left.png" id="left" onmouseover="mouseOver_left()" onmouseout="mouseOut_left()"></a></th><th><a href="https://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/intento1"><img border="0"  src="https://static.igem.org/mediawiki/2009/4/41/Home_LCG_UNAM.png" id="home" onmouseover="mouseOver_home()" onmouseout="mouseOut_home()"></a></th><th><a href="#" onCLick="window.history.go(1); return false;"><img border="0" src="https://static.igem.org/mediawiki/2009/a/af/Flecha_right.png" id="right" onmouseover="mouseOver_right()" onmouseout="mouseOut_right()"></a></th></tr></table>
 +
<a href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:LCG-UNAM-Mexico" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:LCG-UNAM-Mexico" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';"></a>
 +
</body>
 +
</html>
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== Results ==
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<html>
 +
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Revision as of 06:15, 13 October 2009


The Project

Bacteriophage infection represents a common matter in science and industry. We propose to contend with such infections at a population level by triggering a defense system delivered by an engineered version of the satellite phage P4. This phage will be produced into an E. coli strain which harbors some genes from the helper phage P2 that are used for complementing and completing P4 life cycle, hence creating a production line of our version of P4.

This will enable phage production and subsequent delivery of the defense system into wild-type E. coli and possibly other bacterial host strains.

The defense will consist of DNA and RNA degradation by toxins which will be activated by T3 or T7 RNA-Polymerases and transcribed fast enough to stop phage assembly and scattering in the environment.

Moreover, the system includes the spread of a quorum sensing signal, hence "warning" the population to prepare against further T3 or T7 infection.

We will implement a stochastic population model to simulate the infection processes and quantify the efficiency of our system.







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