http://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/Safety&feed=atom&action=historyTeam:LCG-UNAM-Mexico/Safety - Revision history2024-03-28T18:01:55ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/Safety&diff=169058&oldid=prevLawrah: /* Safety */2009-10-22T03:55:19Z<p><span class="autocomment">Safety</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>They think this risk is latent but you can maintain P2 apart from the population and you can screen for the resistance periodically. So, it is possible to control this safety issue. About the E3 toxin they told us that in the laboratory conditions it is not a problem because you work in a restricted environment.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>They think this risk is latent but you can maintain P2 apart from the population and you can screen for the resistance periodically. So, it is possible to control this safety issue. About the E3 toxin they told us that in the laboratory conditions it is not a problem because you work in a restricted environment.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In some literature we have found E3 colicin is able to recognize and cut mouse and HeLa ribosomes[[Team:LCG-UNAM-Mexico/Safety#References|1]] however in other studies they ensure low dosis of these toxin are not so hazardous because of the immune system [[Team:LCG-UNAM-Mexico/Safety#References|2]]. In fact, in a third source they mention this toxin could be used as an anticarcinogenic drug because cancer cells are much more damaged by colicin E3 than normal ones.[[Team:LCG-UNAM-Mexico/Safety#References|3]]<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In some literature we have found E3 colicin is able to recognize and cut mouse and HeLa ribosomes[[Team:LCG-UNAM-Mexico/Safety#References|<ins class="diffchange diffchange-inline">(</ins>1<ins class="diffchange diffchange-inline">)</ins>]] however in other studies they ensure low dosis of these toxin are not so hazardous because of the immune system [[Team:LCG-UNAM-Mexico/Safety#References|<ins class="diffchange diffchange-inline">(</ins>2<ins class="diffchange diffchange-inline">)</ins>]]. In fact, in a third source they mention this toxin could be used as an anticarcinogenic drug because cancer cells are much more damaged by colicin E3 than normal ones.[[Team:LCG-UNAM-Mexico/Safety#References|<ins class="diffchange diffchange-inline">(</ins>3<ins class="diffchange diffchange-inline">)</ins>]]<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Do any of the new BioBrick parts that you made this year raise any safety issues? </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Do any of the new BioBrick parts that you made this year raise any safety issues? <ins class="diffchange diffchange-inline">Yes, the E3 colicin. <br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>o If yes, did you document these issues in the Registry?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>o If yes, did you document these issues in the Registry? <ins class="diffchange diffchange-inline">Yes, we will<br></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>
</table>Lawrahhttp://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/Safety&diff=169003&oldid=prevLawrah: /* Safety */2009-10-22T03:53:16Z<p><span class="autocomment">Safety</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. What does your local biosafety group think about your project? <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3. What does your local biosafety group think about your project? <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>They think this risk is latent but you can maintain P2 apart from the population and you can screen for the resistance periodically. So, it is possible to control this safety issue. About the E3 toxin they told us that in the laboratory conditions it is not a problem because you work in a restricted environment.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>They think this risk is latent but you can maintain P2 apart from the population and you can screen for the resistance periodically. So, it is possible to control this safety issue. About the E3 toxin they told us that in the laboratory conditions it is not a problem because you work in a restricted environment.<ins class="diffchange diffchange-inline"><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In some literature we have found E3 colicin is able to recognize and cut mouse and HeLa ribosomes[[Team:LCG-UNAM-Mexico/Safety#References|1]] however in other studies they ensure low dosis of these toxin are not so hazardous because of the immune system [[Team:LCG-UNAM-Mexico/Safety#References|2]]. In fact, in a third source they mention this toxin could be used as an anticarcinogenic drug because cancer cells are much more damaged by colicin E3 than normal ones.[[Team:LCG-UNAM-Mexico/Safety#References|3]]<br></ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">In some literature have found E3 colicin is able to recognize and cut mouse and HeLa ribosomes(1) however in other studies they ensure low dosis of these toxin are not so hazardous because of the immune system (2). In fact, in a third source they mention this toxin could be used as an anticarcinogenic drug because cancer cells are much more damaged by colicin E3 than normal ones.(3)</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Do any of the new BioBrick parts that you made this year raise any safety issues? </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Do any of the new BioBrick parts that you made this year raise any safety issues? </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>o If yes, did you document these issues in the Registry? </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>o If yes, did you document these issues in the Registry?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>
</table>Lawrahhttp://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/Safety&diff=168998&oldid=prevAbrahamavelar: /* References */2009-10-22T03:53:08Z<p><span class="autocomment">References</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1 <del class="diffchange diffchange-inline">-</del>J. Smarda, et al. “The citotoxic and cytocidal effect of colicon E3 on mammalian tissue cells”. Folia Microbiologica.1978 <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1 J. Smarda, et al. “The citotoxic and cytocidal effect of colicon E3 on mammalian tissue cells”. Folia Microbiologica.1978 <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2 - Viklický V. “The cytoplasmic membrane as a site of the primary effect of colicins on eukaryotic cells”. Folia biologica.1979 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2 - Viklický V. “The cytoplasmic membrane as a site of the primary effect of colicins on eukaryotic cells”. Folia biologica.1979 <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>3 <del class="diffchange diffchange-inline">–J</del>. Smarda, et al. “The action of colicins on eukaryotic cells”. Toxin Reviews. 1983<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>3 <ins class="diffchange diffchange-inline">J</ins>. Smarda, et al. “The action of colicins on eukaryotic cells”. Toxin Reviews. 1983<br></div></td></tr>
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</table>Abrahamavelarhttp://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/Safety&diff=168981&oldid=prevAbrahamavelar: /* References */2009-10-22T03:52:38Z<p><span class="autocomment">References</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1 -J. Smarda, et al. “The citotoxic and cytocidal effect of colicon E3 on mammalian tissue cells”. Folia Microbiologica.1978</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1 -J. Smarda, et al. “The citotoxic and cytocidal effect of colicon E3 on mammalian tissue cells”. Folia Microbiologica.1978 <ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>2 - Viklický V. “The cytoplasmic membrane as a site of the primary effect of colicins on eukaryotic cells”. Folia biologica.1979</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>2 - Viklický V. “The cytoplasmic membrane as a site of the primary effect of colicins on eukaryotic cells”. Folia biologica.1979 <ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>3 –J. Smarda, et al. “The action of colicins on eukaryotic cells”. Toxin Reviews. 1983</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>3 –J. Smarda, et al. “The action of colicins on eukaryotic cells”. Toxin Reviews. 1983<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Teams, please document any answers to these (or other) safety questions in your presentation, wiki presentation, or poster. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!--Do not remove the first and last lines in this page!-->{{Template:LCG_bottom_Netscape}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!--Do not remove the first and last lines in this page!-->{{Template:LCG_bottom_Netscape}}</div></td></tr>
</table>Abrahamavelarhttp://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/Safety&diff=168909&oldid=prevLawrah: /* Safety */2009-10-22T03:50:48Z<p><span class="autocomment">Safety</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>o If yes, did you document these issues in the Registry? </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>o If yes, did you document these issues in the Registry? </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>==References<del class="diffchange diffchange-inline">=</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==References==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1 -J. Smarda, et al. “The citotoxic and cytocidal effect of colicon E3 on mammalian tissue cells”. Folia Microbiologica.1978</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1 -J. Smarda, et al. “The citotoxic and cytocidal effect of colicon E3 on mammalian tissue cells”. Folia Microbiologica.1978</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2 - Viklický V. “The cytoplasmic membrane as a site of the primary effect of colicins on eukaryotic cells”. Folia biologica.1979</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2 - Viklický V. “The cytoplasmic membrane as a site of the primary effect of colicins on eukaryotic cells”. Folia biologica.1979</div></td></tr>
</table>Lawrahhttp://2009.igem.org/wiki/index.php?title=Team:LCG-UNAM-Mexico/Safety&diff=168887&oldid=prevLawrah: New page: {{Template:LCG_top_Netscape}}<!--Do not remove the first and last lines in this page!--> <br> ==Safety== ---- For iGEM 2009 teams are asked to detail how they approached any issues of b...2009-10-22T03:49:56Z<p>New page: {{Template:LCG_top_Netscape}}<!--Do not remove the first and last lines in this page!--> <br> ==Safety== ---- For iGEM 2009 teams are asked to detail how they approached any issues of b...</p>
<p><b>New page</b></p><div>{{Template:LCG_top_Netscape}}<!--Do not remove the first and last lines in this page!--><br />
<br />
<br><br />
==Safety==<br />
<br />
----<br />
<br />
For iGEM 2009 teams are asked to detail how they approached any issues of biological safety associated with their projects. <br><br />
<br />
Specifically, teams should consider the following four questions: <br />
1. Would any of your project ideas raise safety issues in terms of: <br />
*researcher safety, <br><br />
*public safety, or <br><br />
*environmental safety? <br><br />
Maybe, because one of our objectives is to exchange the P2’s region control by an antibiotic resistance, you could confer a resistance to a susceptible bacterial strain. It would happen if your goal was to protect the bacteria against some viruses. Other issue is that maybe the E3 toxin could attack the eucariote ribosomes. <br><br><br />
<br />
2. Is there a local biosafety group, committee, or review board at your institution? <br> Yes, there is.<br><br><br />
<br />
3. What does your local biosafety group think about your project? <br><br />
They think this risk is latent but you can maintain P2 apart from the population and you can screen for the resistance periodically. So, it is possible to control this safety issue. About the E3 toxin they told us that in the laboratory conditions it is not a problem because you work in a restricted environment.<br><br />
<br />
In some literature have found E3 colicin is able to recognize and cut mouse and HeLa ribosomes(1) however in other studies they ensure low dosis of these toxin are not so hazardous because of the immune system (2). In fact, in a third source they mention this toxin could be used as an anticarcinogenic drug because cancer cells are much more damaged by colicin E3 than normal ones.(3)<br />
Do any of the new BioBrick parts that you made this year raise any safety issues? <br />
o If yes, did you document these issues in the Registry? <br />
<br />
===References===<br />
1 -J. Smarda, et al. “The citotoxic and cytocidal effect of colicon E3 on mammalian tissue cells”. Folia Microbiologica.1978<br />
2 - Viklický V. “The cytoplasmic membrane as a site of the primary effect of colicins on eukaryotic cells”. Folia biologica.1979<br />
3 –J. Smarda, et al. “The action of colicins on eukaryotic cells”. Toxin Reviews. 1983<br />
<br />
<br />
Teams, please document any answers to these (or other) safety questions in your presentation, wiki presentation, or poster. <br />
Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety. <br />
If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org.<br />
<br />
<!--Do not remove the first and last lines in this page!-->{{Template:LCG_bottom_Netscape}}</div>Lawrah