Team:LCG-UNAM-Mexico:Journals:Nando's
From 2009.igem.org
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I'll use smaller flasks since the fernbach doesn't fit inside our incubator. | I'll use smaller flasks since the fernbach doesn't fit inside our incubator. | ||
The bacteria grew really slow. That means, about .01 A600 in 3 hours. My best bet will be to leave the culture overnight and see what happened in the morning. | The bacteria grew really slow. That means, about .01 A600 in 3 hours. My best bet will be to leave the culture overnight and see what happened in the morning. | ||
- | I consider the experiment failed, so I'll have to try with a wt bacterium. | + | I consider the experiment failed ([[Team:LCG-UNAM-Mexico:Journals:Nando's#September 19, 2009|check plaque assay on Sep. 19]]), so I'll have to try with a wt bacterium. |
Today I also ran a pcr with various controls and primers. | Today I also ran a pcr with various controls and primers. | ||
only with int P4 primers.- | only with int P4 primers.- | ||
- | CN | + | CN<br> |
- | C1906 colony 2- expected: 3 kb band | + | C1906 colony 2- expected: 3 kb band<br> |
- | C1906 colony 3-expected: 3 kb band | + | C1906 colony 3-expected: 3 kb band<br> |
- | C1906 non-resistant, kit purified from stock-expected:3 kb band | + | C1906 non-resistant, kit purified from stock-expected:3 kb band<br> |
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P4 int and P2 ogr primers.- | P4 int and P2 ogr primers.- | ||
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results.- a strange +- 6 b band appeared in [[Team:LCG-UNAM-Mexico/Resources/Strains|C331]] which twists my mind out. It is possible that I am retrieving a product specific for the primer combination used, so the PCR isn't really valid. Hypotheses are that this newly combined pair of primers | results.- a strange +- 6 b band appeared in [[Team:LCG-UNAM-Mexico/Resources/Strains|C331]] which twists my mind out. It is possible that I am retrieving a product specific for the primer combination used, so the PCR isn't really valid. Hypotheses are that this newly combined pair of primers | ||
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=='''September 10, 2009'''== | =='''September 10, 2009'''== |
Revision as of 00:04, 21 October 2009