http://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&feed=atom&action=historyTeam:Lethbridge/Parts - Revision history2024-03-28T23:37:50ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=167478&oldid=prevAshley.duncan: /* Constructs/Devices */2009-10-22T03:12:30Z<p><span class="autocomment">Constructs/Devices</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:<del class="diffchange diffchange-inline">Theophylline_Induced_chemotaxis2</del>.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:<ins class="diffchange diffchange-inline">Theophylline_Induced_chemotaxis3</ins>.png]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249030: rpsA TIR controlled GFP - Green Fluorescent Protein expression is controlled by the rpsA TIR which allows for the protein to be expressed under stressful cellular conditions. <html><body><span style="color:#FF0000">Cancelled.</span> </body> </html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249030: rpsA TIR controlled GFP - Green Fluorescent Protein expression is controlled by the rpsA TIR which allows for the protein to be expressed under stressful cellular conditions. <html><body><span style="color:#FF0000">Cancelled.</span> </body> </html></div></td></tr>
</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166525&oldid=prevAshley.duncan: /* Constructs/Devices */2009-10-22T02:50:06Z<p><span class="autocomment">Constructs/Devices</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet_inverter.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet_inverter.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249018: N-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249018: N-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet_inverter.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet_inverter.png]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249019: Magnetite nanoparticle Device - This is a composite part. The mms6 enzyme from Magnetospirillum magneticum has been </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249019: Magnetite nanoparticle Device - This is a composite part. The mms6 enzyme from Magnetospirillum magneticum has been </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249023: Targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This device has been designed to show the localization of the yellow fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249023: Targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This device has been designed to show the localization of the yellow fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_YFP_and_lumazine_synthase.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_YFP_and_lumazine_synthase.png]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249024: Targeted C-terminal CFP and targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the co-localization of the Cyan fluorescent Protein and Yellow fluorescent protein both equipped with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249024: Targeted C-terminal CFP and targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the co-localization of the Cyan fluorescent Protein and Yellow fluorescent protein both equipped with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249027: Theophylline Induced GFP - GFP is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing production of GFP. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249027: Theophylline Induced GFP - GFP is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing production of GFP. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_GFP2.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_GFP2.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249028: Theophylline Induced Chemotaxis - Chemotaxis by cheZ is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing the production of cheZ, a key regulatory element in the motility pathway. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249028: Theophylline Induced Chemotaxis - Chemotaxis by cheZ is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing the production of cheZ, a key regulatory element in the motility pathway. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_chemotaxis2.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_chemotaxis2.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249030: rpsA TIR controlled GFP - Green Fluorescent Protein expression is controlled by the rpsA TIR which allows for the protein to be expressed under stressful cellular conditions. <html><body><span style="color:#FF0000">Cancelled.</span> </body> </html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249030: rpsA TIR controlled GFP - Green Fluorescent Protein expression is controlled by the rpsA TIR which allows for the protein to be expressed under stressful cellular conditions. <html><body><span style="color:#FF0000">Cancelled.</span> </body> </html></div></td></tr>
</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166477&oldid=prevAshley.duncan: /* Constructs/Devices */2009-10-22T02:48:59Z<p><span class="autocomment">Constructs/Devices</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:48, 22 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249000: Tet Repressible YFP - This is the Yellow Fluorescent Protein from the Registry (RET) which has been modified with a Tetracycline promoter in order to be (PRET). This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249000: Tet Repressible YFP - This is the Yellow Fluorescent Protein from the Registry (RET) which has been modified with a Tetracycline promoter in order to be (PRET). This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Tet_repressible_YFP.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Tet_repressible_YFP.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249001: Arabinose Induced Inverter - The Tetracycline Inverted has been equipped with the pBAD promoter to allow for Arabinose induced Inversion of which part has been tagged on afterwards. This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249001: Arabinose Induced Inverter - The Tetracycline Inverted has been equipped with the pBAD promoter to allow for Arabinose induced Inversion of which part has been tagged on afterwards. This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Arabinose_induced_inverter.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Arabinose_induced_inverter.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249003: Lumazine Synthase - This is the Composite Part which creates the enzyme Lumazine Synthase. Lumazine Synthase generates the product Lumazine which aggregates together to form a microcompartment, or synthetic organelle. The microcompartment has large negatively charged pores through which proteins containing positively charged Arginine Tags may be targeted. This is a composite part. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249003: Lumazine Synthase - This is the Composite Part which creates the enzyme Lumazine Synthase. Lumazine Synthase generates the product Lumazine which aggregates together to form a microcompartment, or synthetic organelle. The microcompartment has large negatively charged pores through which proteins containing positively charged Arginine Tags may be targeted. This is a composite part. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Lumazine_Synthase3.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Lumazine_Synthase3.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249010: C-terminal Yellow Fluorescent Protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with a c-terminal Arginine tag (10 Additional Arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249010: C-terminal Yellow Fluorescent Protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with a c-terminal Arginine tag (10 Additional Arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249011: C-terminal Cyan Fluorescent Protein with tetracycline promoter - This is a composite part. This Cyan Fluorescent Protein has been fused with a c-terminal Arginine tag (10 additional arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249011: C-terminal Cyan Fluorescent Protein with tetracycline promoter - This is a composite part. This Cyan Fluorescent Protein has been fused with a c-terminal Arginine tag (10 additional arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249012: N-terminal Yellow fluorescent protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249012: N-terminal Yellow fluorescent protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249013: N-terminal Cyan fluorescent protein with tetracycline promoter - This is a composite part. This Cyan fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249013: N-terminal Cyan fluorescent protein with tetracycline promoter - This is a composite part. This Cyan fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249014: C-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249014: C-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline_inverter.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline_inverter.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249015: C-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249015: C-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline_inverter.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline_inverter.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249017: N-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249017: N-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<td colspan="2" class="diff-lineno">Line 806:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. The coding region has been equipped with an IPTG inducible promoter, strong RBS and double terminator. Thank you to the [https://2009.igem.org/Team:Alberta University of Alberta Team]for collaborating with us on this part.<html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. The coding region has been equipped with an IPTG inducible promoter, strong RBS and double terminator. Thank you to the [https://2009.igem.org/Team:Alberta University of Alberta Team]for collaborating with us on this part.<html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Magnetite_nanoparticle_Device.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Magnetite_nanoparticle_Device.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249020: Targeted C-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249020: Targeted C-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_CFP_and_lumazine_synthase.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_CFP_and_lumazine_synthase.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249021: Targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the yellow fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249021: Targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the yellow fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_YFP_and_lumazine_synthase.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_YFP_and_lumazine_synthase.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249022: Targeted N-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249022: Targeted N-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_CFP_and_lumazine_synthase.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_CFP_and_lumazine_synthase.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249023: Targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This device has been designed to show the localization of the yellow fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249023: Targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This device has been designed to show the localization of the yellow fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166416&oldid=prevAshley.duncan: /* Individual Parts */2009-10-22T02:47:34Z<p><span class="autocomment">Individual Parts</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:47, 22 October 2009</td>
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<td colspan="2" class="diff-lineno">Line 521:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249006: Fusion EYFP with C-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has a C-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has a ribosomal binding site. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249006: Fusion EYFP with C-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has a C-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has a ribosomal binding site. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:EYFPCtermfusion_standard.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:EYFPCtermfusion_standard.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249007: Fusion CFP with C-terminal Arginine Tag - This is a composite part. This cyan Fluorescent protein has a c-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has the pTet promoter and a ribosomal binding site. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249007: Fusion CFP with C-terminal Arginine Tag - This is a composite part. This cyan Fluorescent protein has a c-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has the pTet promoter and a ribosomal binding site. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:CFPCtermfusion_standard.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:CFPCtermfusion_standard.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249008: Fusion EYFP with N-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249008: Fusion EYFP with N-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:YFP_Nterm_Fusion.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:YFP_Nterm_Fusion.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249009: Fusion ECFP with N-terminal Arginine Tag - This is a composite part. This Cyan fluorescent protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249009: Fusion ECFP with N-terminal Arginine Tag - This is a composite part. This Cyan fluorescent protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:N-term_fusion_CFP.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:N-term_fusion_CFP.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249016: mms6 - This is a protein coding region. The mms6 enzyme from Magnetospirillum magneticum has been shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249016: mms6 - This is a protein coding region. The mms6 enzyme from Magnetospirillum magneticum has been shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Mms6.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Mms6.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249026 : Theophylline Riboswitch - This is an improvement on the Theophylline Riboswitch submitted by the University of Lethbridge 2007 iGEM team. The theophylline Riboswitch binds to Theophylline in a specific manner to induce transcription of an mRNA. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249026 : Theophylline Riboswitch - This is an improvement on the Theophylline Riboswitch submitted by the University of Lethbridge 2007 iGEM team. The theophylline Riboswitch binds to Theophylline in a specific manner to induce transcription of an mRNA. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Riboswitch.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Riboswitch.png]]</div></td></tr>
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</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166275&oldid=prevAshley.duncan: /* Favourites */2009-10-22T02:44:56Z<p><span class="autocomment">Favourites</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249002: Lumazine Synthase - Lumazine Synthase is an enzyme which creates Lumazine, a product which aggregates forming a hollow spheroid which can act as a mirocompartment, or artificial organelle. The Lumazine forms negatively charged pores, which can be used to introduce proteins. The proteins which are being introduced into the microcompartment must be equipped with an Arginine Tag. This is a protein coding part.<html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249002: Lumazine Synthase - Lumazine Synthase is an enzyme which creates Lumazine, a product which aggregates forming a hollow spheroid which can act as a mirocompartment, or artificial organelle. The Lumazine forms negatively charged pores, which can be used to introduce proteins. The proteins which are being introduced into the microcompartment must be equipped with an Arginine Tag. This is a protein coding part.<html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Lumazine_Synthase2.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Lumazine_Synthase2.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249004: N-terminal Arginine Fusion vector - pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the suffix, and the regular BioBrick standard at the prefix. Adds 10 Arginine residues to the N-terminus of a Protein (includes Start Codon AUG). This part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249004: N-terminal Arginine Fusion vector - pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the suffix, and the regular BioBrick standard at the prefix. Adds 10 Arginine residues to the N-terminus of a Protein (includes Start Codon AUG). This part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_Arg2.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_Arg2.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_Arg2.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_Arg2.png]]</div></td></tr>
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</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166243&oldid=prevAshley.duncan: /* Constructs/Devices */2009-10-22T02:44:26Z<p><span class="autocomment">Constructs/Devices</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249000: Tet Repressible YFP - This is the Yellow Fluorescent Protein from the Registry (RET) which has been modified with a Tetracycline promoter in order to be (PRET). This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249000: Tet Repressible YFP - This is the Yellow Fluorescent Protein from the Registry (RET) which has been modified with a Tetracycline promoter in order to be (PRET). This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Tet_repressible_YFP.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Tet_repressible_YFP.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249001: Arabinose Induced Inverter - The Tetracycline Inverted has been equipped with the pBAD promoter to allow for Arabinose induced Inversion of which part has been tagged on afterwards. This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249001: Arabinose Induced Inverter - The Tetracycline Inverted has been equipped with the pBAD promoter to allow for Arabinose induced Inversion of which part has been tagged on afterwards. This is a composite part. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Arabinose_induced_inverter.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Arabinose_induced_inverter.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249003: Lumazine Synthase - This is the Composite Part which creates the enzyme Lumazine Synthase. Lumazine Synthase generates the product Lumazine which aggregates together to form a microcompartment, or synthetic organelle. The microcompartment has large negatively charged pores through which proteins containing positively charged Arginine Tags may be targeted. This is a composite part. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249003: Lumazine Synthase - This is the Composite Part which creates the enzyme Lumazine Synthase. Lumazine Synthase generates the product Lumazine which aggregates together to form a microcompartment, or synthetic organelle. The microcompartment has large negatively charged pores through which proteins containing positively charged Arginine Tags may be targeted. This is a composite part. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Lumazine_Synthase3.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Lumazine_Synthase3.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249010: C-terminal Yellow Fluorescent Protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with a c-terminal Arginine tag (10 Additional Arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249010: C-terminal Yellow Fluorescent Protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with a c-terminal Arginine tag (10 Additional Arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249011: C-terminal Cyan Fluorescent Protein with tetracycline promoter - This is a composite part. This Cyan Fluorescent Protein has been fused with a c-terminal Arginine tag (10 additional arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249011: C-terminal Cyan Fluorescent Protein with tetracycline promoter - This is a composite part. This Cyan Fluorescent Protein has been fused with a c-terminal Arginine tag (10 additional arginine residues at the c-terminus) and has been fixed with a tetracycline promoter, mRBS and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249012: N-terminal Yellow fluorescent protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249012: N-terminal Yellow fluorescent protein with tetracycline promoter - This is a composite part. This Yellow fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249013: N-terminal Cyan fluorescent protein with tetracycline promoter - This is a composite part. This Cyan fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249013: N-terminal Cyan fluorescent protein with tetracycline promoter - This is a composite part. This Cyan fluorescent Protein has been fused with an N-terminal Arginine tag (10 Additional Arginine residues at the N-terminus) and has been fixed with a tetracycline promoter, mRBS, and double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249014: C-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249014: C-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline_inverter.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_YFP_tetracycline_inverter.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249015: C-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249015: C-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with C-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline_inverter.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_CFP_tetracycline_inverter.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249017: N-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249017: N-terminal Yellow fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of YFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet_inverter.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_YFP_tet_inverter.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249018: N-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249018: N-terminal Cyan fluorescent protein with tetracycline inverter - Arabinose repressible Tetracycline inversion of CFP with N-terminal Fusion Arganine Tag for targeting into Lumazine Microcompartment. Has a medium RBS and a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet_inverter.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Nterm_CFP_tet_inverter.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249019: Magnetite nanoparticle Device - This is a composite part. The mms6 enzyme from Magnetospirillum magneticum has been </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249019: Magnetite nanoparticle Device - This is a composite part. The mms6 enzyme from Magnetospirillum magneticum has been </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. The coding region has been equipped with an IPTG inducible promoter, strong RBS and double terminator. Thank you to the [https://2009.igem.org/Team:Alberta University of Alberta Team]for collaborating with us on this part.<html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. The coding region has been equipped with an IPTG inducible promoter, strong RBS and double terminator. Thank you to the [https://2009.igem.org/Team:Alberta University of Alberta Team]for collaborating with us on this part.<html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Magnetite_nanoparticle_Device.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Magnetite_nanoparticle_Device.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249020: Targeted C-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249020: Targeted C-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_CFP_and_lumazine_synthase.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_CFP_and_lumazine_synthase.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249021: Targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the yellow fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249021: Targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the yellow fluorescent Protein with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_YFP_and_lumazine_synthase.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_cterm_YFP_and_lumazine_synthase.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249022: Targeted N-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249022: Targeted N-terminal CFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the localization of the Cyan fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_CFP_and_lumazine_synthase.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_CFP_and_lumazine_synthase.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249023: Targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This device has been designed to show the localization of the yellow fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249023: Targeted N-terminal YFP and Lumazine Synthase - This is a composite part. This device has been designed to show the localization of the yellow fluorescent Protein with N-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_YFP_and_lumazine_synthase.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Targeted_nterm_YFP_and_lumazine_synthase.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249024: Targeted C-terminal CFP and targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the co-localization of the Cyan fluorescent Protein and Yellow fluorescent protein both equipped with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249024: Targeted C-terminal CFP and targeted C-terminal YFP and Lumazine Synthase - This is a composite part. This is device has been designed to show the co-localization of the Cyan fluorescent Protein and Yellow fluorescent protein both equipped with C-terminal Arginine tag into the Lumazine Microcompartment. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 833:</td>
<td colspan="2" class="diff-lineno">Line 834:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249027: Theophylline Induced GFP - GFP is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing production of GFP. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249027: Theophylline Induced GFP - GFP is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing production of GFP. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_GFP2.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_GFP2.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249028: Theophylline Induced Chemotaxis - Chemotaxis by cheZ is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing the production of cheZ, a key regulatory element in the motility pathway. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249028: Theophylline Induced Chemotaxis - Chemotaxis by cheZ is controlled through translation by the Theophylline Riboswitch. When Theophylline is present, the Riboswitch undergoes a conformational change to reveal the RBS, allowing the production of cheZ, a key regulatory element in the motility pathway. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_chemotaxis2.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Theophylline_Induced_chemotaxis2.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249030: rpsA TIR controlled GFP - Green Fluorescent Protein expression is controlled by the rpsA TIR which allows for the protein to be expressed under stressful cellular conditions. <html><body><span style="color:#FF0000">Cancelled.</span> </body> </html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249030: rpsA TIR controlled GFP - Green Fluorescent Protein expression is controlled by the rpsA TIR which allows for the protein to be expressed under stressful cellular conditions. <html><body><span style="color:#FF0000">Cancelled.</span> </body> </html></div></td></tr>
</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166172&oldid=prevAshley.duncan: /* Favourites */2009-10-22T02:43:13Z<p><span class="autocomment">Favourites</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:43, 22 October 2009</td>
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<td colspan="2" class="diff-lineno">Line 310:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>[[Image:Cterm_Arg2.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Cterm_Arg2.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166156&oldid=prevAshley.duncan: /* Favourites */2009-10-22T02:42:46Z<p><span class="autocomment">Favourites</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:42, 22 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>[[Image:Cterm_Arg2.png<del class="diffchange diffchange-inline">|center</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>[[Image:Cterm_Arg2.png]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166119&oldid=prevAshley.duncan: /* Individual Parts */2009-10-22T02:42:17Z<p><span class="autocomment">Individual Parts</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:42, 22 October 2009</td>
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<td colspan="2" class="diff-lineno">Line 521:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249006: Fusion EYFP with C-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has a C-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has a ribosomal binding site. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249006: Fusion EYFP with C-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has a C-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has a ribosomal binding site. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:EYFPCtermfusion_standard.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:EYFPCtermfusion_standard.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249007: Fusion CFP with C-terminal Arginine Tag - This is a composite part. This cyan Fluorescent protein has a c-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has the pTet promoter and a ribosomal binding site. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249007: Fusion CFP with C-terminal Arginine Tag - This is a composite part. This cyan Fluorescent protein has a c-terminal fusion suffix (BioFusion AKA Silver Lab standard) and is ready to be fused to the C-terminal Arginine tag. This part has the pTet promoter and a ribosomal binding site. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:CFPCtermfusion_standard.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:CFPCtermfusion_standard.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249008: Fusion EYFP with N-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249008: Fusion EYFP with N-terminal Arginine Tag - This is a composite part. This Yellow Fluorescent Protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249009: Fusion ECFP with N-terminal Arginine Tag - This is a composite part. This Cyan fluorescent protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249009: Fusion ECFP with N-terminal Arginine Tag - This is a composite part. This Cyan fluorescent protein has an N-terminal fusion prefix (BioFusion AKA Silver Lab standard) and is ready to be fused to the N-terminal Arginine tag. This part has a double terminator. <html> <body><span style="color:#330570">In progress</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249016: mms6 - This is a protein coding region. The mms6 enzyme from Magnetospirillum magneticum has been shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249016: mms6 - This is a protein coding region. The mms6 enzyme from Magnetospirillum magneticum has been shown to produce uniform Magnetite nanoparticles when in the presence of an Iron Chloride based solution. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Mms6.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Mms6.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249026 : Theophylline Riboswitch - This is an improvement on the Theophylline Riboswitch submitted by the University of Lethbridge 2007 iGEM team. The theophylline Riboswitch binds to Theophylline in a specific manner to induce transcription of an mRNA. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249026 : Theophylline Riboswitch - This is an improvement on the Theophylline Riboswitch submitted by the University of Lethbridge 2007 iGEM team. The theophylline Riboswitch binds to Theophylline in a specific manner to induce transcription of an mRNA. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Riboswitch.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Riboswitch.png<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
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</table>Ashley.duncanhttp://2009.igem.org/wiki/index.php?title=Team:Lethbridge/Parts&diff=166093&oldid=prevAshley.duncan: /* Favourites */2009-10-22T02:41:49Z<p><span class="autocomment">Favourites</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:41, 22 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BBa_K249005: C-terminal Arginine fusion vector - pSB1A3 with a C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly at the prefix and the regular Biobrick standard at the suffix. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon) this part is meant to work in conjunction with the Lumazine synthase microcompartment. Protein with the N-terminal Arginine fusion will be localized into the negative core of the Lumazine microcompartments. <html> <body><span style="color:#04346C">Submitted.</span> </body> </html> </div></td></tr>
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