http://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&feed=atom&action=historyTeam:Michigan/Project - Revision history2024-03-29T13:37:45ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=163311&oldid=prevAnnlesn at 01:21, 22 October 20092009-10-22T01:21:30Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Toluene Terminator is a ''Pseudomonas putida'' device that aims to identify the toxic compound toluene in an environmental setting (e.g. a spill into soil from an underground petrol tank), move to and uptake it, metabolize it, and destroy itself when all of the toluene has been metabolized. This device will require four basic modules: toluene chemorecognition, chemotaxis, toluene metabolism, and a suicide mechanism. This year we <del class="diffchange diffchange-inline">have been working </del>on toluene metabolism and the suicide mechanism.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Toluene Terminator is a ''Pseudomonas putida'' device that aims to identify the toxic compound toluene in an environmental setting (e.g. a spill into soil from an underground petrol tank), move to and uptake it, metabolize it, and destroy itself when all of the toluene has been metabolized. This device will require four basic modules: toluene chemorecognition, chemotaxis, toluene metabolism, and a suicide mechanism. This year we <ins class="diffchange diffchange-inline">focused </ins>on toluene metabolism and the suicide mechanism.</div></td></tr>
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</table>Annlesnhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=163298&oldid=prevAnnlesn at 01:21, 22 October 20092009-10-22T01:21:02Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==<B><font size=5>Chemotaxis</font></B>==</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We wanted to link chemotaxis to our project to have the the bacteria seek out toluene in contaminated environments. We planned on engineering ''P. putida'' to swim up toluene gradients. We did a few initial studies to characterize the natural motility of ''P. putida'' and our results showed that ''P. putida'' has chemotaxis abilities. After this first initial study we focused on the other parts of the project. </ins></div></td></tr>
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</table>Annlesnhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=159293&oldid=prevAnnlesn at 23:18, 21 October 20092009-10-21T23:18:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The goal of this project is to work with the toluene degrading abilities that already exist in ''P. putida'' mt-2 (ATCC 33015) on the pWW0-TOL plasmid. The pathway on this plasmid is comprised of the upper pathway, where toluene is metabolized into catechol, and the lower meta-pathway, where catechol is converted into acetaldehyde and pyruvate. The regulation of toluene degradation on the pWW0 plasmid is presented below. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The goal of this project is to work with the toluene degrading abilities that already exist in ''P. putida'' mt-2 (ATCC 33015) on the pWW0-TOL plasmid. The pathway on this plasmid is comprised of the upper pathway, where toluene is metabolized into catechol, and the lower meta-pathway, where catechol is converted into acetaldehyde and pyruvate. The regulation of toluene degradation on the pWW0 plasmid is presented below. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:<del class="diffchange diffchange-inline">Toluene_degradation_topology</del>.jpg|950px]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:<ins class="diffchange diffchange-inline">Toluene_degradation_topology2</ins>.jpg|950px]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The image is adapted from Burlage, R. S., S. W. Hooper, and G. S. Sayler. 1989. The TOL (pWWO) catabolic plasmid. Appl. Environ. Microbiol. 55: 1323-1328.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The image is adapted from Burlage, R. S., S. W. Hooper, and G. S. Sayler. 1989. The TOL (pWWO) catabolic plasmid. Appl. Environ. Microbiol. 55: 1323-1328.</div></td></tr>
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</table>Annlesnhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=158777&oldid=prevIfaulknr at 23:02, 21 October 20092009-10-21T23:02:30Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our project is aimed at characterizing the promoters in the pWW0 plasmid to sense when the toluene degrading process is finished, signaling the cell to commit suicide. The promoter we chose to sense the presence of toluene is the Pu promoter. In order to characterize this promoter we created <del class="diffchange diffchange-inline">Bba_K270003</del>, a device that has the Pu promoter expressing GFP. To have this device function in strains other than ''P. putida'' mt-2 that already contains the XylR regulating protein, the part <del class="diffchange diffchange-inline">Bba_K270001 </del>was created from the Pr XylR portion of the pWW0 plasmid to regulate the Pu promoter. By combining the functions of these two parts, the intensity of GFP can be used to measure the promoter activity when induced with non-lethal level of toluene. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our project is aimed at characterizing the promoters in the pWW0 plasmid to sense when the toluene degrading process is finished, signaling the cell to commit suicide. The promoter we chose to sense the presence of toluene is the Pu promoter. In order to characterize this promoter we created <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K270003<font color=navy><B>Bba K270003</B></font>]</ins>, a device that has the Pu promoter expressing GFP. To have this device function in strains other than ''P. putida'' mt-2 that already contains the XylR regulating protein, the part <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K270001<font color=navy><B>Bba K270001</B></font>] </ins>was created from the Pr XylR portion of the pWW0 plasmid to regulate the Pu promoter. By combining the functions of these two parts, the intensity of GFP can be used to measure the promoter activity when induced with non-lethal level of toluene. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Currently, we have made the Pu GFP and Pr XylR parts. In the future, we plan on creating one device composed of these two parts and testing the Promoter activity in both ''E. coli'' and ''P. putida''. With this data we will have a better idea of how to link the toluene sensing ability of the Pu promoter with suicide mechanism. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Currently, we have made the <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K270003<font color=navy><B></ins>Pu GFP<ins class="diffchange diffchange-inline"></B></font>] </ins>and <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K270001<font color=navy><B></ins>Pr XylR<ins class="diffchange diffchange-inline"></B></font>] </ins>parts. In the future, we plan on creating one device composed of these two parts and testing the Promoter activity in both ''E. coli'' and ''P. putida''. With this data we will have a better idea of how to link the toluene sensing ability of the Pu promoter with suicide mechanism. </div></td></tr>
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</table>Ifaulknrhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=158458&oldid=prevIfaulknr at 22:54, 21 October 20092009-10-21T22:54:13Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Toluene Terminator is a ''Pseudomonas'' device that aims to identify the toxic compound toluene in an environmental setting (e.g. a spill into soil from an underground petrol tank), move to and uptake it, metabolize it, and destroy itself when all of the toluene has been metabolized. This device will require four basic modules: toluene chemorecognition, chemotaxis, toluene metabolism, and a suicide mechanism. This year we have been working on toluene metabolism and the suicide mechanism.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Toluene Terminator is a ''Pseudomonas <ins class="diffchange diffchange-inline">putida</ins>'' device that aims to identify the toxic compound toluene in an environmental setting (e.g. a spill into soil from an underground petrol tank), move to and uptake it, metabolize it, and destroy itself when all of the toluene has been metabolized. This device will require four basic modules: toluene chemorecognition, chemotaxis, toluene metabolism, and a suicide mechanism. This year we have been working on toluene metabolism and the suicide mechanism.</div></td></tr>
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</table>Ifaulknrhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=158365&oldid=prevIfaulknr at 22:52, 21 October 20092009-10-21T22:52:03Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The goal of this project is to work with the toluene degrading abilities that already exist in ''P. putida'' mt-2 (ATCC 33015) on the pWW0-TOL plasmid. The pathway on this plasmid is comprised of the upper pathway where toluene is metabolized into catechol and the lower<del class="diffchange diffchange-inline">, </del>meta-pathway where catechol is converted into acetaldehyde and pyruvate. The regulation of toluene degradation on the pWW0 plasmid is presented below. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The goal of this project is to work with the toluene degrading abilities that already exist in ''P. putida'' mt-2 (ATCC 33015) on the pWW0-TOL plasmid. The pathway on this plasmid is comprised of the upper pathway<ins class="diffchange diffchange-inline">, </ins>where toluene is metabolized into catechol<ins class="diffchange diffchange-inline">, </ins>and the lower meta-pathway<ins class="diffchange diffchange-inline">, </ins>where catechol is converted into acetaldehyde and pyruvate. The regulation of toluene degradation on the pWW0 plasmid is presented below. </div></td></tr>
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</table>Ifaulknrhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=158159&oldid=prevIfaulknr at 22:46, 21 October 20092009-10-21T22:46:36Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<B><font size=<del class="diffchange diffchange-inline">4</del>>Overview</font></B>== </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<B><font size=<ins class="diffchange diffchange-inline">5</ins>>Overview</font></B>== </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<B><font size=<del class="diffchange diffchange-inline">4</del>>Toluene Metabolism</font></B>== </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<B><font size=<ins class="diffchange diffchange-inline">5</ins>>Toluene Metabolism</font></B>== </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<B><font size=<del class="diffchange diffchange-inline">4</del>>Suicide Mechanism</font></B>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<B><font size=<ins class="diffchange diffchange-inline">5</ins>>Suicide Mechanism</font></B>==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. These two kill switches could both appear, with others, in a final device, considering that severalfold kill switch redundancy will be necessary to prevent "runaway Terminators" (see Safety page). In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. These two kill switches could both appear, with others, in a final device, considering that severalfold kill switch redundancy will be necessary to prevent "runaway Terminators" (see Safety page). In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In this design, a repression mechanism is downstream of the Pu promoter, and this this represses the production of holin and lysozyme proteins. A constitutive promoter is placed in the front of the antiholin gene so antiholin is constantly produced. When toluene is present, it activates the Pu promoter which results in the repression of the promoter expressing holin and lysozyme. As a result, the cell survives. However, in the absence of toluene, the repressor protein is not produced, so holin and lysozyme levels rise to the point that the cells are lysed. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In this design, a repression mechanism is downstream of the Pu promoter, and this this represses the production of holin and lysozyme proteins. A constitutive promoter is placed in the front of the antiholin gene so antiholin is constantly produced. When toluene is present, it activates the Pu promoter which results in the repression of the promoter expressing holin and lysozyme. As a result, the cell survives. However, in the absence of toluene, the repressor protein is not produced, so holin and lysozyme levels rise to the point that the cells are lysed. <ins class="diffchange diffchange-inline">This design is fairly tunable because we can choose which repression system to use, through which we can tweak the holin-antiholin balance.</ins></div></td></tr>
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</table>Ifaulknrhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=157859&oldid=prevIfaulknr at 22:38, 21 October 20092009-10-21T22:38:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==<B><font size=4>Suicide Mechanism</font></B>==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==<B><font size=4>Suicide Mechanism</font></B>==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. <ins class="diffchange diffchange-inline">These two kill switches could both appear, with others, in a final device, considering that severalfold kill switch redundancy will be necessary to prevent "runaway Terminators" (see Safety page). </ins>In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=<font color=royalblue size=3>Arabinose <del class="diffchange diffchange-inline">Inducible </del>Suicide Mechanism</font>=</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=<font color=royalblue size=3>Arabinose<ins class="diffchange diffchange-inline">-Dependent </ins>Suicide Mechanism</font>=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font color=navy></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font color=navy></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The </del>suicide operon consists of an arabinose-dependent promoter controlling expression of the holin and lysozyme proteins, followed by the Pu promoter controlling the antiholin gene shown in the figure below. This way, none of the genes are activated until the device is released into the contaminated area, along with a quantity of arabinose. This results in the arabinose-mediated expression of the lytic genes holin and lysozyme, as well as the toluene-mediated expression of antiholin, which posttranslationally inhibits the lytic proteins. Then, when the toluene disappears (when it is all metabolized) from the local region of the individual device, antiholin is no longer expressed, allowing the lytic proteins to destroy the cell.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">This </ins>suicide operon consists of an arabinose-dependent promoter controlling expression of the holin and lysozyme proteins, followed by the Pu promoter controlling the antiholin gene shown in the figure below. This way, none of the genes are activated until the device is released into the contaminated area, along with a quantity of arabinose. This results in the arabinose-mediated expression of the lytic genes holin and lysozyme, as well as the toluene-mediated expression of antiholin, which posttranslationally inhibits the lytic proteins. Then, when the toluene disappears (when it is all metabolized) from the local region of the individual device, antiholin is no longer expressed, allowing the lytic proteins to destroy the cell.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The advantage of this design is the cells can be stored in the <del class="diffchange diffchange-inline">absense </del>of arabinose without worrying about expressing the holin and lysozyme. When used in an actual bioremediation application, the arabinose promoter could be changed to a substrate that is already present in the contaminated environment to trigger cell death. This would also help prevent cells from poliferating in the environment after release. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The advantage of this design is the cells can be stored in the <ins class="diffchange diffchange-inline">absence </ins>of arabinose without worrying about expressing the holin and lysozyme. When used in an actual bioremediation application, the arabinose promoter could be changed to a substrate that is already present in the contaminated environment to trigger cell death. This would also help prevent cells from poliferating in the environment after release. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Kill_switch_topology2.jpg|950px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Kill_switch_topology2.jpg|950px]]</div></td></tr>
</table>Ifaulknrhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=156761&oldid=prevAnnlesn at 21:59, 21 October 20092009-10-21T21:59:39Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==<B><font size=4>Suicide Mechanism</font></B>==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==<B><font size=4>Suicide Mechanism</font></B>==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font<ins class="diffchange diffchange-inline">></ins>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=<font color=royalblue size=3>Arabinose Inducible Suicide Mechanism</font>=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=<font color=royalblue size=3>Arabinose Inducible Suicide Mechanism</font>=</div></td></tr>
</table>Annlesnhttp://2009.igem.org/wiki/index.php?title=Team:Michigan/Project&diff=156746&oldid=prevAnnlesn at 21:59, 21 October 20092009-10-21T21:59:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==<B><font size=4>Suicide Mechanism</font></B>==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==<B><font size=4>Suicide Mechanism</font></B>==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font<del class="diffchange diffchange-inline">></del>] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Terminator's suicide mechanism, or kill switch, operates through the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808<font color=navy><B>Enterobacteria phage T4 Lysis Device</B></font] created by the Berkley 2008 team. We proposed two mechanisms for cell lysis: arabinose inducible suicide mechanism and suicide mechanism with tunable repression. In the both models the Pu promoter is controlling the cell survival and we make the assumption that when the cells are used for bioremediation purposes they are placed in a toluene-contaminated environment. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=<font color=royalblue size=3>Arabinose Inducible Suicide Mechanism</font>=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=<font color=royalblue size=3>Arabinose Inducible Suicide Mechanism</font>=</div></td></tr>
</table>Annlesn