Team:MoWestern Davidson/project wetlab

From 2009.igem.org

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We began our project design by constructing 12 suppressor tRNAs by oligo assembly. Suppressor tRNA genes were ligated with the pBad promoter to allow weak constitutive and inducible tRNA expression.
We began our project design by constructing 12 suppressor tRNAs by oligo assembly. Suppressor tRNA genes were ligated with the pBad promoter to allow weak constitutive and inducible tRNA expression.
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[[Image:Pbad trna.JPG]]
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[[Image:Pbad trna.JPG|thumb|none|300px]]
[[Image:Pbadrbsreporter.JPG]]
[[Image:Pbadrbsreporter.JPG]]
[[Image:Pbadrbsreportertrna.JPG]]
[[Image:Pbadrbsreportertrna.JPG]]

Revision as of 16:09, 27 July 2009

We began our project design by constructing 12 suppressor tRNAs by oligo assembly. Suppressor tRNA genes were ligated with the pBad promoter to allow weak constitutive and inducible tRNA expression.

Pbad trna.JPG

Pbadrbsreporter.JPG

Pbadrbsreportertrna.JPG