Team:NTU-Singapore/Notebook

From 2009.igem.org

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(June)
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* Antibiotic stock preparation
* Antibiotic stock preparation
* Inoculation
* Inoculation
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<u>'''20th  June'''</u>
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* Our first Plasmid Extraction (PE)!
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<u>'''22nd June'''</u>
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* First Double Digestion (DD) and Gel Check!
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<u>'''23rd June '''</u>
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* PE & DD of the registry constructs….
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<u>'''24th June '''</u>
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*Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).
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<u>'''26th June'''</u>
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*PE , DD and Gel check for the direct synthesized gene sequence.
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<u>'''27th June  '''</u>
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*Gel Check for CHE, IFP and pNO
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==July==
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<u>'''  '''</u>
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*
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<u>'''  '''</u>
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*
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<u>'''  '''</u>
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*
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<u>'''  '''</u>
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*
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<u>'''  '''</u>
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Revision as of 19:07, 21 October 2009

Experiment Timeline


June

18th June

  • Preparation of LB medium
  • Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)


19th June

  • Antibiotic stock preparation
  • Inoculation


20th June

  • Our first Plasmid Extraction (PE)!


22nd June

  • First Double Digestion (DD) and Gel Check!


23rd June

  • PE & DD of the registry constructs….


24th June

  • Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).


26th June

  • PE , DD and Gel check for the direct synthesized gene sequence.


27th June

  • Gel Check for CHE, IFP and pNO


July








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