Revision as of 19:07, 21 October 2009 by Sjee (Talk | contribs)

Experiment Timeline


18th June

  • Preparation of LB medium
  • Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)

19th June

  • Antibiotic stock preparation
  • Inoculation

20th June

  • Our first Plasmid Extraction (PE)!

22nd June

  • First Double Digestion (DD) and Gel Check!

23rd June

  • PE & DD of the registry constructs….

24th June

  • Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).

26th June

  • PE , DD and Gel check for the direct synthesized gene sequence.

27th June

  • Gel Check for CHE, IFP and pNO


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