Revision as of 19:10, 21 October 2009 by Sjee (Talk | contribs)

Experiment Timeline


18th June

  • Preparation of LB medium
  • Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)

19th June

  • Antibiotic stock preparation
  • Inoculation

20th June

  • Our first Plasmid Extraction (PE)!

22nd June

  • First Double Digestion (DD) and Gel Check!

23rd June

  • PE & DD of the registry constructs….

24th June

  • Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).

26th June

  • PE , DD and Gel check for the direct synthesized gene sequence.

27th June

  • Gel Check for CHE, IFP and pNO


1st July

  • PE for plaqIQ, J23101, J23119, B0034(rbs), B0015(Term-T for short), B0034-GFP-B0015

2nd July

  • PE for pNO, IFP, CHE
  • DD of pNO, J23119, J23101, rbs-GFP-T
  • Our first ligation!

3rd July

  • DD of CHE, IFP and B0015, plaqIQ and plux.
  • Ligation.

4th July

  • PE: pNO-GFP; J23101-GFP; J23119-GFP; HO1
  • DD: HO1

NTU@iGEMcc 2009. Some rights reserved.