Team:NTU-Singapore/Notebook

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Experiment Timeline


June

18th June

  • Preparation of LB medium
  • Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)


19th June

  • Antibiotic stock preparation
  • Inoculation


20th June

  • Our first Plasmid Extraction (PE)!


22nd June

  • First Double Digestion (DD) and Gel Check!


23rd June

  • PE & DD of the registry constructs….


24th June

  • Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).


26th June

  • PE , DD and Gel check for the direct synthesized gene sequence.


27th June

  • Gel Check for CHE, IFP and pNO


July

1st July

  • PE for plaqIQ, J23101, J23119, B0034(rbs), B0015(Term-T for short), B0034-GFP-B0015


2nd July

  • PE for pNO, IFP, CHE
  • DD of pNO, J23119, J23101, rbs-GFP-T
  • Our first ligation!


3rd July

  • DD of CHE, IFP and B0015, plaqIQ and plux.
  • Ligation.


4th July

  • PE: pNO-GFP; J23101-GFP; J23119-GFP; HO1
  • DD: HO1






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