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| - | <div id="plaquebanner" class="clearfix">
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| - | <div id="ntuproject" class="grid_16">
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| - | <span class="hidden">Welcome to the project page!</span>
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| - | <div id="menu" class="hp clearfix main"><div class="grid_4 link"><span class="gray">Hard at work!</span></div><div class="grid_1 link">[[Team:NTU-Singapore | Home]]</div><div class="grid_2 link selflink">[[Team:NTU-Singapore/Project | <span class="selflink">pLaq''U''<span class="sup">e</span> Out!</span>]]</div><div class="grid_3 link">[[Team:NTU-Singapore/HPA | Human Practice]]</div><div class="grid_2 link">[[Team:NTU-Singapore/Notebook | Notebook]]</div><div class="grid_2 link">[[Team:NTU-Singapore/About/Team | About Us]]</div><div class="grid_2 link contact">[[Team:NTU-Singapore/Contact | Contact Us!]]</div></div>
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| - | <div id="ntucontent"><div class="wrap_3 suffix_1" id="submenu">
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| - | <span>Navigation</span>
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| - | [[Team:NTU-Singapore/Project | Why Atherosclerosis?]]
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| - | [[Team:NTU-Singapore/Project/System | System Specifications]]
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| - | [[Team:NTU-Singapore/Project/Prototype | Prototype System]]
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| - | [[Team:NTU-Singapore/Project/Proposal | Research proposal]]
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| - | [[Team:NTU-Singapore/Project/Prototype/Sense | Sense Device « ]]
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| - | [[Team:NTU-Singapore/Project/Prototype/Degrade | Degradation Device « ]]
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| - | [[Team:NTU-Singapore/Project/Prototype/Image | Imaging Device « ]]
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| - | [[Team:NTU-Singapore/Project/Prototype/Dilate | Dilation Device « ]]
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| - | [[Team:NTU-Singapore/Parts | Parts]]
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| - | [[Team:NTU-Singapore/Project/References | References]]
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| - | [[Team:NTU-Singapore/Project/Conclusion | Conclusion]]
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| - | <span class="title">Our <span class="bold">Prototype</span></span><br class="clear" />
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| - | __TOC__
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| - | In the [[Team:NTU-Singapore/Project/System | previous section]], we have covered the systemized our objectives for use in a biological ''machine''. We have also further broken the system down into specific devices.
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| - | Due to time and equipment constraints, we will not detail the design of the ideal system, since we will not be able to build it anyway. Instead, we will focus on designing a full-blown prototype system as a proof-of-concept, using E.Coli as our model organism.<br class="clear" />
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| - | == Prototype Design ==
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| - | Recall the construct layout for the ideal system.
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| - | [[Image:NTUsysdev.gif|center]]
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| - | Our prototype will utilize a similar layout. We will detail the '''parts''' we have identified for our devices.
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| - | '''Sense device''' :
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| - | * Arguably the most important gene sequence, we have decided to use the NO-sensitive promoter region of the NorV/W gene sequence endogeneous in E.Coli.
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| - | ** This NO-sensitive promoter (we will refer to it as pNO henceforth)
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