Team:NYMU-Taipei/Project

From 2009.igem.org

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[[Image:NYMU Wt-po.png]]
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== Project Overview ==
== Project Overview ==
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Our ViroTerminator is made Safe for the bloodstream. When it is injected into the bloodstream, our ViroTerminator passively lies around in the blood stream letting viruses attach to it by using its 4 receptors: CD4 (for HIV), Integrin (for various viruses), Sialic Acid (for Influenza), Antibodies (specific chosen viruses). After enough viruses attach to it, or after a certain amount of time elapses, it removes itself from the bloodstream by calling macrophages to eat it up.
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# The objective: Binding viruses to designer ViroCatcher cells that cannot support viral replication to diagnose, attenuate, and prevent infection.
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# What we intended to do: (1) Make our designer cell safe, (2) Express specific cell surface receptors and antibodies to catch the virus, (3) Transduce the signal after viruses attached for feedback control, and (4) Remove the viruses along with ViroCatcher itself.
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# Anticipated results: the ViroCatcher is made safe for the bloodstream. When it is injected into the bloodstream, our ViroCatcher passively lies around letting viruses attach to it by using its 4 receptors: CD4 (for HIV), Integrin (for various viruses), Sialic Acid (for Influenza), and Antibodies (for Influenza). After enough viruses attach to it, or after a certain amount of time elapses, it removes itself from the bloodstream by calling macrophages to eat it up.
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== Sales Pitch ==
== Sales Pitch ==
<html><div style="width:50%;margin-left:25%;border:2px solid black;padding:20px;"></html>
<html><div style="width:50%;margin-left:25%;border:2px solid black;padding:20px;"></html>
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<span style="font-weight:900;font-size:24px;font-family:helvetica;"><center>ViroCatcher</center></span>
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<span style="font-weight:900;font-size:36px;font-family:bank gothic;"><center>ViroCatcher</center></span>
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<br>
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Want to be virus-free<sup>[1]</sup>? Our ViroCatcher destroys viruses ranging from short-term viruses such as the flu, to long term viruses such as HIV. Buy our ViroTerminator today!
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Want to be <span style="color:#ff6600">virus-free</span><sup>[1]</sup>? Our ViroCatcher destroys viruses ranging from short-term viruses such as the flu, to long term viruses such as HIV!
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<br>
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<br>
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<span style="font-weight:bold;font-size:28px;font-style:italic;font-family:Chalkboard;"><center>Buy our ViroCatcher today!</center></span>
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<html><font size="1">
<html><font size="1">
Small print:
Small print:
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<br>[1] <s>Free as in free beer.</s> Free from viruses that the ViroTerminator is designed to act upon.
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<br>[1] <s>Free as in free beer.</s> Free from viruses that the ViroCatcher is designed to act upon.
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== Design ==
== Design ==
Our design was split into 4 parts:
Our design was split into 4 parts:
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* Making our machine safe
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* Designing the chassis
* Attaching receptors to catch the virus
* Attaching receptors to catch the virus
* Signal Transduction after viruses attach
* Signal Transduction after viruses attach
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* Removal of the ViroTerminator itself and the virus
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* Removal of the ViroCatcher itself and the virus
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This research is aimed to design a method to capture the specific membrane protein of virus.  For certain virus, we design the biological parts, the conjugated viral receptor, to bind onto it and trigger LPS for removal.  Also a backup evacuation, oscillator triggered removal, will act when it catches no virus for a certain time.
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== Delegation ==
== Delegation ==
Our subteams for experimentation and research were split in this manner:
Our subteams for experimentation and research were split in this manner:
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* Safe E. coli: Making our machine safe.
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* Safe ''E. coli'': Making our machine safe.
* Cellular receptors
* Cellular receptors
** CD4 (HIV)
** CD4 (HIV)
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* Removal
* Removal
* Testing
* Testing
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== Acknowledgements ==
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*Dr. Ching-Hao Teng, NCKU, for providing us ''E. coli'' K1 for experiment
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{{:Team:NYMU-Taipei/Footer}}
{{:Team:NYMU-Taipei/Footer}}

Revision as of 19:50, 21 October 2009

NYMU Wt-po.png

Contents

Project Overview

  1. The objective: Binding viruses to designer ViroCatcher cells that cannot support viral replication to diagnose, attenuate, and prevent infection.
  2. What we intended to do: (1) Make our designer cell safe, (2) Express specific cell surface receptors and antibodies to catch the virus, (3) Transduce the signal after viruses attached for feedback control, and (4) Remove the viruses along with ViroCatcher itself.
  3. Anticipated results: the ViroCatcher is made safe for the bloodstream. When it is injected into the bloodstream, our ViroCatcher passively lies around letting viruses attach to it by using its 4 receptors: CD4 (for HIV), Integrin (for various viruses), Sialic Acid (for Influenza), and Antibodies (for Influenza). After enough viruses attach to it, or after a certain amount of time elapses, it removes itself from the bloodstream by calling macrophages to eat it up.

Sales Pitch

ViroCatcher


Want to be virus-free[1]? Our ViroCatcher destroys viruses ranging from short-term viruses such as the flu, to long term viruses such as HIV!

Buy our ViroCatcher today!



Small print:
[1] Free as in free beer. Free from viruses that the ViroCatcher is designed to act upon.

Design

Our design was split into 4 parts:

  • Designing the chassis
  • Attaching receptors to catch the virus
  • Signal Transduction after viruses attach
  • Removal of the ViroCatcher itself and the virus

Delegation

Our subteams for experimentation and research were split in this manner:

  • Safe E. coli: Making our machine safe.
  • Cellular receptors
    • CD4 (HIV)
    • Integrin (various)
    • Sialic Acid (Influenza)
    • Antibodies (specific)
  • Signal Transduction
  • Removal
  • Testing

Acknowledgements

  • Dr. Ching-Hao Teng, NCKU, for providing us E. coli K1 for experiment

Retrieved from "http://2009.igem.org/Team:NYMU-Taipei/Project"