Team:Nevada/Project

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== Project Details==
== Project Details==
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The goal of our project is to clone one or more genes from the cynnamaldehyde pathway into E. coli as a potential insecticide against mosquito larvae. The ultimate goal of this project would be to move one or more of these genes into Wolfia, a small aquatic plant to which mosquito larvae feed upon during the spring and summer months.
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=== Goal 1 ===
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The initial goal of this project is to clone the Arabidopsis gene cynnamaldehyde CoA-reductase into an E. coli expression system and test for activity. Currently, we are planning to use an inducible promoter, a RBS, and double terminator from the standard registry of parts to create this construct.
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=== Part 2 ===
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=== The Experiments ===
=== The Experiments ===
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1. Conduct a three way ligation with pBAD (or LacI), RBS and a chloromphenicol resistant plasmid backbone (recombinant 1).
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2. Conduct a three way ligation with cynnamaldehyde CoA, double terminator and chloromphenicol resistant plasmid (recombinant 2). 
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3. Conduct a three way ligation with recombinant 1, 2 and a tetracycline resestant plasmid.
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=== Goal 2 ===
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=== Part 3 ===
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== Results ==
== Results ==

Revision as of 22:46, 1 July 2009

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The goal of our project is to clone one or more genes from the cynnamaldehyde pathway into E. coli as a potential insecticide against mosquito larvae. The ultimate goal of this project would be to move one or more of these genes into Wolfia, a small aquatic plant to which mosquito larvae feed upon during the spring and summer months.

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Project Details

The goal of our project is to clone one or more genes from the cynnamaldehyde pathway into E. coli as a potential insecticide against mosquito larvae. The ultimate goal of this project would be to move one or more of these genes into Wolfia, a small aquatic plant to which mosquito larvae feed upon during the spring and summer months.

Goal 1

The initial goal of this project is to clone the Arabidopsis gene cynnamaldehyde CoA-reductase into an E. coli expression system and test for activity. Currently, we are planning to use an inducible promoter, a RBS, and double terminator from the standard registry of parts to create this construct.


The Experiments

1. Conduct a three way ligation with pBAD (or LacI), RBS and a chloromphenicol resistant plasmid backbone (recombinant 1). 2. Conduct a three way ligation with cynnamaldehyde CoA, double terminator and chloromphenicol resistant plasmid (recombinant 2). 3. Conduct a three way ligation with recombinant 1, 2 and a tetracycline resestant plasmid.

Goal 2

Results