Team:Nevada/Project

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Contents

Overall project

Your abstract




Project Details

The goal of our project is to clone one or more genes from the cinnamaldehyde pathway into E. coli as a potential insecticide against mosquito larvae. The ultimate goal of this project would be to move one or more of these genes into Wolffia, a small aquatic plant to which mosquito larvae feed upon during the spring and summer months.

Why Mosquitoes?

180px-Aedes aegypti biting human.jpg

It is estimated that every year over 700 million people are infected by diseases spread by mosquitoes, and over 2 million of them die as a result. Control of mosquito populations in a cost effective and environmentally safe way is one of many ways to help lower the casualties.

Goal 1

The initial goal of this project is to clone the Arabidopsis gene cinnamaldehyde CoA-reductase into an E. coli expression system and test for activity. Currently, we are planning to use an inducible promoter, a RBS, and double terminator from the standard registry of parts to create this construct.


The Experiments

1. Conduct a three way ligation with pBAD (or LacI), RBS and a chloromphenicol resistant plasmid backbone (recombinant 1). 2. Conduct a three way ligation with cinnamaldehyde CoA, double terminator and chloromphenicol resistant plasmid (recombinant 2). 3. Conduct a three way ligation with recombinant 1, 2 and a tetracycline resestant plasmid.

Goal 2

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Results