Team:Nevada/Team

From 2009.igem.org

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(Where we're from)
(What we did)
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== '''What we did''' ==
== '''What we did''' ==
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[[Janice Cho and Leigh Armijo]]
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[[Janice Cho]]
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- Transformed and extracted DNA samples for lacI promoter, ribosome-binding site, double terminator, and various plasmid backbones.
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- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
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- Performed three-way ligation for the lacI promoter, ribsome-binding site, and a chloramphenicol resistant plasmid backbone.
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- Helped conduct a three-way ligation to create final construct with the BBa_K262000 and BBa_K262002.   
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- Transformed mutated 4-coumarate:CoA ligase gene into DH5-alpha competent ''E. Coli'' cells.   
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[[Leigh Armijo]]
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- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
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- Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.
[[Sheena Jones]]
[[Sheena Jones]]

Revision as of 00:23, 20 October 2009



Back: Dr. Shintani, J, Alex, Tyler. Middle: Janice, Leigh, Joey, Chris. Front: Nick and Dr. Howard

Contents

Who we are

 

Advisors

  • Advisor 1: Dr. Christie Howard
  • Advisor 2: Dr. Patricia Ellison
  • Advisor 3: Dr. Dave Shintani
  • Grad Student 1: Chad Sanada


Undergrads

  • Student 1: Nick Tschernia
  • Student 2: Tony Moschetti
  • Student 3: Joey Khwaja
  • Student 4: Chris Martinez
  • Student 5: Janice Cho
  • Student 6: Leigh Armijo
  • Student 7: Sheena Jones
  • Student 8: Tyler Peterson
  • Student 9: J Malikowski
  • Student 10: Alex Dussaq


What we did

Janice Cho - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. - Helped conduct a three-way ligation to create final construct with the BBa_K262000 and BBa_K262002.

Leigh Armijo - Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. - Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.

Sheena Jones


J Malikowski -Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future. -Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps. -Trained Tyler to be an excellent Molecular Biologist. -Advisory role at lab meetings (How does the Harper lab do it?)

Tyler Peterson -Major player in media preparation, agarose gels, stock solutions -Helped with minipreps and E. coli transformation.

Chris Martinez -Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species -Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector -Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species

Where we're from

alt text
Nick Tschernia
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Tony Moschetti
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Janice Cho
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Leigh Armijo
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Joey Khwaja
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Sheena Jones
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Chris Martinez
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Tyler Peterson
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J Malikowski
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Alex Dussaq
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Chad Sanada
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Dr. Christie Howard
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Dr. Dave Shintani
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Dr. Trish Ellison