Team:Nevada/Team

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Back: Dr. Shintani, J, Alex, Tyler. Middle: Janice, Leigh, Joey, Chris. Front: Nick and Dr. Howard

Contents

Who we are

 

Advisors

  • Advisor 1: Dr. Christie Howard
  • Advisor 2: Dr. Patricia Ellison
  • Advisor 3: Dr. Dave Shintani
  • Grad Student 1: Chad Sanada


Undergrads

  • Student 1: Nick Tschernia
  • Student 2: Tony Moschetti
  • Student 3: Joey Khwaja
  • Student 4: Chris Martinez
  • Student 5: Janice Cho
  • Student 6: Leigh Armijo
  • Student 7: Sheena Jones
  • Student 8: Tyler Peterson
  • Student 9: J Malikowski
  • Student 10: Alex Dussaq


What we did

Nick Tschernia

  • Entered the Nevada Team into the iGEM Competition a week before the submission date.
  • Organized weekly meetings for project brainstorming and timeline.
  • Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions).
  • Organized the source plate distribution and transformation of the basic parts.
  • Sequence analysis of the Biobrick parts formed.
  • Primer design for adding Biobrick restriction sites for formation of Biobrick parts.


Janice Cho

  • Formulated the original idea of using cinnamaldehyde as a mosquito larvicide.
  • Performed initial research on the use of cinnamaldehyde.
  • Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
  • Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002.


Leigh Armijo

  • Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
  • Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.


Sheena Jones

  • In charge of maintaining and propagating Wolffia and Lemna plant species.
  • Generated callus culture in preparation for gene transformation.


Tony Moschetti

  • Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part.
  • Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002)
  • Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones.


J Malikowski

  • Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future.
  • Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
  • Trained Tyler to be an excellent Molecular Biologist.
  • Advisory role at lab meetings (How does the Harper lab do it?)


Tyler Peterson

  • Major player in media preparation, agarose gels, stock solutions
  • Helped with minipreps and E. coli transformation.


Chris Martinez

  • Made db3.1 and NEB10 competent cells.
  • Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species
  • Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
  • Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species


Joey Khwaja

  • Made db3.1 and NEB10 competent cells.
  • Researched into making a Biobrick plant part.
  • Designed the iGEM Nevada Team shirts.

Meet the Team

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Nick Tschernia
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Tony Moschetti
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Janice Cho
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Leigh Armijo
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Joey Khwaja
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Sheena Jones
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Chris Martinez
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Tyler Peterson
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J Malikowski
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Alex Dussaq
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Chad Sanada
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Dr. Christie Howard
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Dr. Dave Shintani
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Dr. Trish Ellison