http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&feed=atom&action=historyTeam:Newcastle/Stochasticity - Revision history2024-03-28T17:17:13ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=165282&oldid=prevJanehsy: /* Introduction */2009-10-22T02:23:47Z<p><span class="autocomment">Introduction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Newcastle <del class="diffchange diffchange-inline">Animation Stoachstic Switch</del>.gif|center]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Newcastle <ins class="diffchange diffchange-inline">r Switchv2</ins>.gif|center]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td></tr>
</table>Janehsyhttp://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=165218&oldid=prevJanehsy: /* Introduction */2009-10-22T02:22:07Z<p><span class="autocomment">Introduction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Newcastle Animation Stoachstic Switch.gif<del class="diffchange diffchange-inline">|500px</del>|center]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Newcastle Animation Stoachstic Switch.gif|center]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td></tr>
</table>Janehsyhttp://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=164757&oldid=prevJanehsy: /* Introduction */2009-10-22T02:08:58Z<p><span class="autocomment">Introduction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Newcastle Animation Stoachstic Switch.gif]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Newcastle Animation Stoachstic Switch.gif<ins class="diffchange diffchange-inline">|500px|center</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td></tr>
</table>Janehsyhttp://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=164619&oldid=prevJanehsy: /* Introduction */2009-10-22T02:04:48Z<p><span class="autocomment">Introduction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[Image:Newcastle Animation Stoachstic Switch.gif]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[</ins>[Image:Newcastle Animation Stoachstic Switch.gif]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td></tr>
</table>Janehsyhttp://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=164595&oldid=prevBabyneurone: /* Introduction */2009-10-22T02:03:42Z<p><span class="autocomment">Introduction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[</del>[Image:Newcastle Animation Stoachstic Switch.gif<del class="diffchange diffchange-inline">|center|500px</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[Image:Newcastle Animation Stoachstic Switch.gif]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td></tr>
</table>Babyneuronehttp://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=164403&oldid=prevBabyneurone: /* Introduction */2009-10-22T01:57:09Z<p><span class="autocomment">Introduction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By differentially controlling the expression of the Hin invertase, we designed our switch to be tunable to achieve a biased heads or tails response, allowing a range of probabilities of orientation of the invertable segment to be achieved.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Newcastle Animation Stoachstic Switch.gif|center|500px]]</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Novelty in this sub-project==</div></td></tr>
</table>Babyneuronehttp://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=162336&oldid=prevNaw3: /* BioBrick constructs */2009-10-22T00:53:07Z<p><span class="autocomment">BioBrick constructs</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are a number of bricks involved within the stochastic switch construct.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are a number of bricks involved within the stochastic switch construct.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The stochastic brick construct uses the Hin invertase system in order to flip a region between Hix sites. The directionality of the promoter determines whether the switch is 'on' or 'off'. When the promoter is facing right it allows transcription of genes that control</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The stochastic brick construct uses the Hin invertase system in order to flip a region between Hix sites. The directionality of the promoter determines whether the switch is 'on' or 'off'. When the promoter is facing right it allows transcription of genes that control<ins class="diffchange diffchange-inline">:</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Prevention of germination </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Prevention of germination </div></td></tr>
</table>Naw3http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=162315&oldid=prevNaw3: /* BioBrick constructs */2009-10-22T00:52:40Z<p><span class="autocomment">BioBrick constructs</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are a number of bricks involved within the stochastic switch construct.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are a number of bricks involved within the stochastic switch construct.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The stochastic brick construct uses the Hin invertase system in order to flip a region between Hix sites. The directionality of the promoter determines whether the switch is 'on' or 'off'. When the promoter is facing right it allows transcription of genes that control<del class="diffchange diffchange-inline">:</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The stochastic brick construct uses the Hin invertase system in order to flip a region between Hix sites. The directionality of the promoter determines whether the switch is 'on' or 'off'. When the promoter is facing right it allows transcription of genes that control</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Prevention of germination </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Prevention of germination </div></td></tr>
</table>Naw3http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=162281&oldid=prevNaw3: /* BioBrick constructs */2009-10-22T00:51:40Z<p><span class="autocomment">BioBrick constructs</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Importantly, Hin is '''differentially expressed''' depending on the levels of the two inducible promoters that flank the invertable segment on which it lies. This means the segment can be '''<del class="diffchange diffchange-inline">baised</del>''' in a predictable and controllable fashion to favour one orientation or the other. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Importantly, Hin is '''differentially expressed''' depending on the levels of the two inducible promoters that flank the invertable segment on which it lies. This means the segment can be '''<ins class="diffchange diffchange-inline">biased</ins>''' in a predictable and controllable fashion to favour one orientation or the other. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Naw3http://2009.igem.org/wiki/index.php?title=Team:Newcastle/Stochasticity&diff=162264&oldid=prevNaw3: /* BioBrick constructs */2009-10-22T00:51:12Z<p><span class="autocomment">BioBrick constructs</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The stochastic brick construct uses the Hin invertase system in order to flip a region between Hix sites. The directionality of the promoter determines whether the switch is 'on' or 'off'. When the promoter is facing right it allows transcription of genes that control:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The stochastic brick construct uses the Hin invertase system in order to flip a region between Hix sites. The directionality of the promoter determines whether the switch is 'on' or 'off'. When the promoter is facing right it allows transcription of genes that control:</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Prevention of germination </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Prevention of germination </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Upregulation of sporulation rate</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Upregulation of sporulation rate</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 27:</td>
<td colspan="2" class="diff-lineno">Line 29:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Decreased cadmium efflux</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Decreased cadmium efflux</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Upregulation of cadmium import</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** Upregulation of cadmium import</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Importantly, Hin is '''differentially expressed''' depending on the levels of the two inducible promoters that flank the invertable segment on which it lies. This means the segment can be '''baised''' in a predictable and controllable fashion to favour one orientation or the other. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The following diagram shows our stochastic construct:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The following diagram shows our stochastic construct:</div></td></tr>
</table>Naw3