PCR Sequencing

Sensor Experiment 1: Receivers vs AHL
Procedure:
1. Pre-culture cells transformed with parts to be tested in solution medium overnight.
2. Inoculate fresh medium with pre-cultured cells and culture till full growth.
3. Replace culture medium by ultracentrifuging and resuspending cells in fresh medium.
4. Add AHL to culture.
5. Measure OD and fluorescence at regular intervals.
[link to protocol]

Sensor Experiment 2: Senders vs Receivers
Procedure:
1. Pre-culture cells transformed with parts to be tested in solution medium overnight.
2. Pipette fresh medium into microcentrifuge tubes then inoculate with cells from overnight culture.
3. Add AHL for the Receivers-only samples.
4. Culture overnight at 37 degrees Celsius in incubator.
5. Measure fluorescence.
[link to protocol]