Notebook > AND_Gate_1 > AraC SupD Assembly
2009.8.3
00:00
Ligation:
Insert: pcr1, pla1
Vector: SupD, 1-12O
12:00
Transformation: yesterday’s ligation product
21:45
Grow the strains of pla1+SupD1, pcr1+1-12O1~4, pla1+1-12O1~4
22:10
Ligation:
Insert: pcr1
Vector: SupD
2009.8.4
11:00
Transformation: pcr1+SupD
13:00
Mini-prep: pla+1-12O1~4, pcr+1-12O1~4, plas1
18:30
Double-enzyme digestion: pla+1-12O3, pcr+1-12O1, pla+SupD1
22:30
Double-enzyme digestion: pla+1-12O3, pcr+1-12O1
2009.8.5
11:00
Sent samples for sequencing
16:00
Ligation:
Insert: pcr1, pla2
Vector: 1-12O
2009.8.6
15:00
Inducing test
Strain: pla+1-12O1, pcr+1-12O1
15:30
Double-enzyme digestion
Plasmid: pla+1-12O2, pcr+1-12O2
2009.8.7
15:00
Double-enzyme digestion: pcr1~6
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