Team:PKU Beijing/Notebook/Assembly/Rencheng Gao

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Revision as of 21:04, 21 October 2009

Notebook

Notes By Person (PDF)

Notebook > Assembly > Assembling : bistable-> pSB1A2 & pSB6A1

2009.10.1

10:00
Double-enzyme digestion:

Plasmid(bistable on pSB4K3)	5μl
SpeI	1μl
PstI	1μl
Buffer H	2μl
ddH2O	11μl

14:00
Gel purification

16:00
Ligation:
Vector: Bistable
Insert: T7-CI(1H, 2M, 2I, 2G, 2K, 1J)
Ligase: 1μl
Buffer: 1μl

18:00
Transformation

2009.10.2

10:00
Bacteria culture:
Bistable-T7p-CI(1H, 2M, 2I, 2G, 5N, 1J)

24:00
Mini-prep:
Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

2009.10.3

09:00
Double-enzyme digestion: Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

16:00
Ligation:
Vector: Bistable on 1O and on 2K
Insert: T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

20:00
Transformation

2009.10.4

09:00
Bacteria culture:
1O-Bistable-T7p-CI-2K, 1O-Bistable-T7p-CI-2G, 1O-Bistable-T7p-CI-2M, 12K-Bistable-T7p-CI-2I, 12K-Bistable-T7p-CI-1H, 12K-Bistable-T7p-CI-1J

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@@ Line 59: / Line 59: @@
 '''09:00'''<br>
 Bacteria culture:<br>
 O-Bistable-T7p-CI-2K, 1O-Bistable-T7p-CI-2G, 1O-Bistable-T7p-CI-2M, 12K-Bistable-T7p-CI-2I, 12K-Bistable-T7p-CI-1H, 12K-Bistable-T7p-CI-1J
 {{PKU_Beijing/Foot}}
 __NOTOC__