Team:PKU Beijing/Project

From 2009.igem.org

(Difference between revisions)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
-
{{PKU_Beijing/Blue}}
+
{{PKU_Beijing/Header}}
-
{{PKU_Beijing/Style}}
+
{{PKU_Beijing/Sidebar_Project}}
-
<html>
+
{{PKU_Beijing/Header2}}
-
<table width="965" border="0" cellspacing="0" cellpadding="0">
+
==='''Idea'''===
-
  <tr bgcolor="#0E7AD3">
+
-
    <td height="40" colspan="3">
+
-
    <img src='https://static.igem.org/mediawiki/2009/6/65/PKU_The_Project.png'>
+
-
    </td>
+
-
  </tr>
+
-
  <tr bgcolor="#0E7AD3">
+
-
    <td height="20" colspan="3">&nbsp;</td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td width="50" bgcolor="#0E7AD3">&nbsp;</td>
+
-
    <td width="865" bgcolor="#0E7AD3">
+
-
<table width="865" border="0" cellspacing="0" cellpadding="0">
+
-
  <tr>
+
-
    <td width="16"><img src="https://static.igem.org/mediawiki/2009/5/54/PKU_Upperleft.png" /></td>
+
-
    <td width="833"></td>
+
-
    <td width="16"><img src="https://static.igem.org/mediawiki/2009/6/62/PKU_Upperright.png" /></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td width="16"></td>
+
-
    <td width="833"></html>Back to the beginning of 20th century, Ivan Pavlov brought up the concept "conditioned reflex".
+
-
The famous work is done on dog. Ring bell is supposed to be a conditioned stimulus to the dog, when given the ring, no salivation happens to the dog. A training session, in which both the ring and the food are given to the dog, helps the dog build a relationship between the ring bell and the food. After that, the ring bell alone can cause salivation also. This work not only earned Pavlov the 1904 Nobel Prize in Physiology and Medicine, but is also a milestone for the research of learning and cognition.
+
{{PKU_Beijing/Idea}}
-
Digging into the mechanism of the Conditioned Reflex (The memory of Correlation in the brain), it is thought that simultaneous activity of two neurons can bring them together. Hebbian Rule of Firing together, Wiring together is among the most famous theories. It is also the basis of “Artificial Neural Network”, which modifies the connectivity of two nodes in the network according to the concurrence of activity. Connectivity increases if the two nodes are activated or depressed at the same time, else, the connectivity is weakened. The molecular support of this theory is conformed when the phenomenon of “Long Term Potentiation” is discovered.
+
==='''Project Abstract'''===
-
Here in PKU iGEM2009 Team, we want to build E.coli version of the classical “conditioned reflex” experiment.<html>
+
We are engineering our E. coli cells to process the correlation information of two enviornmental signal, similar to the process of conditioning in higher orgamisms. We have constructed and tested a series of AND gates which can sense the two signals: the conditioned and unconditioned stimuli. With the presence of both signals, the AND gate outputs a repressor protein and then changes the state of the bistable switch, which acts as a memory module. In this way, our E. coli cells can convert the information about the concurrence of the two signals into its memory. After the memory module is switched and given the "conditioned stimulus", the E. coli cells will pass the information to the reporter module and thus exhibit the "conditioned response."
-
</td>
+
 
-
    <td width="16"></td>
+
{{PKU_Beijing/Foot}}
-
  </tr>
+
-
  <tr>
+
-
    <td width="16"><img src="https://static.igem.org/mediawiki/2009/3/32/PKU_Downleft.png" /></td>
+
-
    <td width="833"></td>
+
-
    <td width="16"><img src="https://static.igem.org/mediawiki/2009/0/09/PKU_Downright.png" /></td>
+
-
  </tr>
+
-
</table>
+
-
    </td>
+
-
    <td width="50" bgcolor="#0E7AD3">&nbsp;</td>
+
-
  </tr>
+
-
  <tr bgcolor="#0E7AD3">
+
-
    <td height="20" colspan="3">&nbsp;</td>
+
-
  </tr>
+
-
</table>
+
-
</html>
+

Latest revision as of 07:29, 15 October 2009

 

Idea

iCEM logo

The Idea to make a E.coli that can memorize the correlationship between two signals like classical Conditioning is proposed by Longwen Huang. Huang is an undergraduate from Yuanpei College, Peking University

Project Abstract

We are engineering our E. coli cells to process the correlation information of two enviornmental signal, similar to the process of conditioning in higher orgamisms. We have constructed and tested a series of AND gates which can sense the two signals: the conditioned and unconditioned stimuli. With the presence of both signals, the AND gate outputs a repressor protein and then changes the state of the bistable switch, which acts as a memory module. In this way, our E. coli cells can convert the information about the concurrence of the two signals into its memory. After the memory module is switched and given the "conditioned stimulus", the E. coli cells will pass the information to the reporter module and thus exhibit the "conditioned response."



^Top